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Title: Fatty alcohol production in Lipomyces starkeyi and Yarrowia lipolytica

Abstract

Current biological pathways to produce biofuel intermediates amenable to separations and catalytic upgrading to hydrocarbon fuels are not cost effective. Previously, oleaginous yeasts have been investigated primarily for lipid production. However, yeasts store neutral lipids intracellularly making recovery difficult and expensive. In addition, once recovered from the cells, lipids are difficult to blend directly with the existing fuels without upgrading. We have, therefore, begun to investigate secreted fatty acid-derived products which can be easily recovered and upgraded to fuels. In this study, we successfully demonstrate the production of fatty alcohols by the oleaginous yeasts, Yarrowia lipolytica and Lipomyces starkeyi, through expression of the fatty acyl-CoA reductase gene from Marinobactor aquaeolei VT8. This strategy resulted in the production of 167 and 770 mg/L of fatty alcohols in shake flask from Y. lipolytica and L starkeyi, respectively. When using a dodecane overlay during fermentation, 92 and 99% of total fatty alcohols produced by Y. lipolytica and L. starkeyi, respectively, were extracted into the dodecane phase, which compares favorably to the 3 and 50% recovered, respectively, without the dodecane layer. In both oleaginous yeasts, long chain length, saturated fatty alcohols, i.e., hexadecanol (C16:0) and octadecanol (C18:0), were predominant and accounted for more thanmore » 85% of the total fatty alcohols produced. To the best of our knowledge, this is the first report of fatty alcohol production in L. starkeyi. Furthermore, this work demonstrates that the oleaginous yeasts, Y. lipolytica and L. starkeyi, can serve as platform organisms for the production of fatty acid-derived biofuels and bioproducts.« less

Authors:
; ; ; ; ; ;
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office
OSTI Identifier:
1618653
Alternate Identifier(s):
OSTI ID: 1332481
Report Number(s):
NREL/JA-2700-65740
Journal ID: ISSN 1754-6834; 227; PII: 647
Grant/Contract Number:  
AC36-08-GO28308; AC36-08GO28308
Resource Type:
Published Article
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Name: Biotechnology for Biofuels Journal Volume: 9 Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
Springer Science + Business Media
Country of Publication:
Netherlands
Language:
English
Subject:
09 BIOMASS FUELS; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; metabolic engineering; oleaginous yeasts; Yarrowia lipolytica; Lipomyces starkeyi; fatty alcohols

Citation Formats

Wang, Wei, Wei, Hui, Knoshaug, Eric, Van Wychen, Stefanie, Xu, Qi, Himmel, Michael E., and Zhang, Min. Fatty alcohol production in Lipomyces starkeyi and Yarrowia lipolytica. Netherlands: N. p., 2016. Web. https://doi.org/10.1186/s13068-016-0647-2.
Wang, Wei, Wei, Hui, Knoshaug, Eric, Van Wychen, Stefanie, Xu, Qi, Himmel, Michael E., & Zhang, Min. Fatty alcohol production in Lipomyces starkeyi and Yarrowia lipolytica. Netherlands. https://doi.org/10.1186/s13068-016-0647-2
Wang, Wei, Wei, Hui, Knoshaug, Eric, Van Wychen, Stefanie, Xu, Qi, Himmel, Michael E., and Zhang, Min. Mon . "Fatty alcohol production in Lipomyces starkeyi and Yarrowia lipolytica". Netherlands. https://doi.org/10.1186/s13068-016-0647-2.
@article{osti_1618653,
title = {Fatty alcohol production in Lipomyces starkeyi and Yarrowia lipolytica},
author = {Wang, Wei and Wei, Hui and Knoshaug, Eric and Van Wychen, Stefanie and Xu, Qi and Himmel, Michael E. and Zhang, Min},
abstractNote = {Current biological pathways to produce biofuel intermediates amenable to separations and catalytic upgrading to hydrocarbon fuels are not cost effective. Previously, oleaginous yeasts have been investigated primarily for lipid production. However, yeasts store neutral lipids intracellularly making recovery difficult and expensive. In addition, once recovered from the cells, lipids are difficult to blend directly with the existing fuels without upgrading. We have, therefore, begun to investigate secreted fatty acid-derived products which can be easily recovered and upgraded to fuels. In this study, we successfully demonstrate the production of fatty alcohols by the oleaginous yeasts, Yarrowia lipolytica and Lipomyces starkeyi, through expression of the fatty acyl-CoA reductase gene from Marinobactor aquaeolei VT8. This strategy resulted in the production of 167 and 770 mg/L of fatty alcohols in shake flask from Y. lipolytica and L starkeyi, respectively. When using a dodecane overlay during fermentation, 92 and 99% of total fatty alcohols produced by Y. lipolytica and L. starkeyi, respectively, were extracted into the dodecane phase, which compares favorably to the 3 and 50% recovered, respectively, without the dodecane layer. In both oleaginous yeasts, long chain length, saturated fatty alcohols, i.e., hexadecanol (C16:0) and octadecanol (C18:0), were predominant and accounted for more than 85% of the total fatty alcohols produced. To the best of our knowledge, this is the first report of fatty alcohol production in L. starkeyi. Furthermore, this work demonstrates that the oleaginous yeasts, Y. lipolytica and L. starkeyi, can serve as platform organisms for the production of fatty acid-derived biofuels and bioproducts.},
doi = {10.1186/s13068-016-0647-2},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 9,
place = {Netherlands},
year = {2016},
month = {10}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1186/s13068-016-0647-2

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Cited by: 10 works
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