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Title: Viscous control of cellular respiration by membrane lipid composition

Abstract

Lipid composition determines the physical properties of biological membranes and can vary substantially between and within organisms. We describe a specific role for the viscosity of energy-transducing membranes in cellular respiration. Engineering of fatty acid biosynthesis in Escherichia coli allowed us to titrate inner membrane viscosity across a 10-fold range by controlling the abundance of unsaturated or branched lipids. These fluidizing lipids tightly controlled respiratory metabolism, an effect that can be explained with a quantitative model of the electron transport chain (ETC) that features diffusion-coupled reactions between enzymes and electron carriers (quinones). Lipid unsaturation also modulated mitochondrial respiration in engineered budding yeast strains. Thus, diffusion in the ETC may serve as an evolutionary constraint for lipid composition in respiratory membranes.

Authors:
ORCiD logo; ORCiD logo; ORCiD logo; ; ORCiD logo; ORCiD logo
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1484838
Alternate Identifier(s):
OSTI ID: 1656478
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Published Article
Journal Name:
Science
Additional Journal Information:
Journal Name: Science Journal Volume: 362 Journal Issue: 6419; Journal ID: ISSN 0036-8075
Publisher:
AAAS
Country of Publication:
United States
Language:
English

Citation Formats

Budin, Itay, de Rond, Tristan, Chen, Yan, Chan, Leanne Jade G., Petzold, Christopher J., and Keasling, Jay D. Viscous control of cellular respiration by membrane lipid composition. United States: N. p., 2018. Web. doi:10.1126/science.aat7925.
Budin, Itay, de Rond, Tristan, Chen, Yan, Chan, Leanne Jade G., Petzold, Christopher J., & Keasling, Jay D. Viscous control of cellular respiration by membrane lipid composition. United States. doi:10.1126/science.aat7925.
Budin, Itay, de Rond, Tristan, Chen, Yan, Chan, Leanne Jade G., Petzold, Christopher J., and Keasling, Jay D. Thu . "Viscous control of cellular respiration by membrane lipid composition". United States. doi:10.1126/science.aat7925.
@article{osti_1484838,
title = {Viscous control of cellular respiration by membrane lipid composition},
author = {Budin, Itay and de Rond, Tristan and Chen, Yan and Chan, Leanne Jade G. and Petzold, Christopher J. and Keasling, Jay D.},
abstractNote = {Lipid composition determines the physical properties of biological membranes and can vary substantially between and within organisms. We describe a specific role for the viscosity of energy-transducing membranes in cellular respiration. Engineering of fatty acid biosynthesis in Escherichia coli allowed us to titrate inner membrane viscosity across a 10-fold range by controlling the abundance of unsaturated or branched lipids. These fluidizing lipids tightly controlled respiratory metabolism, an effect that can be explained with a quantitative model of the electron transport chain (ETC) that features diffusion-coupled reactions between enzymes and electron carriers (quinones). Lipid unsaturation also modulated mitochondrial respiration in engineered budding yeast strains. Thus, diffusion in the ETC may serve as an evolutionary constraint for lipid composition in respiratory membranes.},
doi = {10.1126/science.aat7925},
journal = {Science},
number = 6419,
volume = 362,
place = {United States},
year = {2018},
month = {10}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1126/science.aat7925

Citation Metrics:
Cited by: 32 works
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