Structure of the Fc fragment of the NIST reference antibody RM8671

Journal Article · 29 August 2018 · Acta Crystallographica. Section F, Structural Biology Communications

DOI: https://doi.org/10.1107/S2053230X18009834 · OSTI ID:1467531

Gallagher, D. Travis; Galvin, Connor V.; Karageorgos, Ioannis

As the link between antigen binding and immune activation, the antibody Fc region has received extensive structural study. In this report, the structure of the Fc fragment of the NIST IgG1 mAb (reference material 8671) is described at 2.1 Å resolution in space group P 2 ₁ 2 ₁ 2 ₁ , with approximate unit-cell parameters a  = 50, b = 80, c = 138 Å. Prior Fc structures with a wide variety of modifications are also surveyed, focusing on those in the same crystal form. To facilitate the analysis of conformations, a reference frame and a two-parameter metric are proposed, considering the CH2 domains as mobile with respect to a fixed dimeric CH3 core. Over several human Fc structures, a significant variation in Fc elbow conformations is observed, which may serve to facilitate the regulation of Fc effector signaling.

• Sponsoring Organization: USDOE
• Grant/Contract Number: AC02-06CH11357
• OSTI ID: 1467531
• Journal Information: Acta Crystallographica. Section F, Structural Biology Communications, Journal Name: Acta Crystallographica. Section F, Structural Biology Communications Vol. 74 Journal Issue: 9; ISSN 2053-230X
• Publisher: International Union of Crystallography (IUCr)Copyright Statement
• Country of Publication: United Kingdom
• Language: English