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Title: Structure of the Fc fragment of the NIST reference antibody RM8671

Abstract

As the link between antigen binding and immune activation, the antibody Fc region has received extensive structural study. In this report, the structure of the Fc fragment of the NIST IgG1 mAb (reference material 8671) is described at 2.1 Å resolution in space group P 2 1 2 1 2 1 , with approximate unit-cell parameters a  = 50, b = 80, c = 138 Å. Prior Fc structures with a wide variety of modifications are also surveyed, focusing on those in the same crystal form. To facilitate the analysis of conformations, a reference frame and a two-parameter metric are proposed, considering the CH2 domains as mobile with respect to a fixed dimeric CH3 core. Over several human Fc structures, a significant variation in Fc elbow conformations is observed, which may serve to facilitate the regulation of Fc effector signaling.

Authors:
; ;
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1467531
Grant/Contract Number:  
AC02-06CH11357
Resource Type:
Published Article
Journal Name:
Acta Crystallographica. Section F, Structural Biology Communications
Additional Journal Information:
Journal Name: Acta Crystallographica. Section F, Structural Biology Communications Journal Volume: 74 Journal Issue: 9; Journal ID: ISSN 2053-230X
Publisher:
International Union of Crystallography (IUCr)
Country of Publication:
United Kingdom
Language:
English

Citation Formats

Gallagher, D. Travis, Galvin, Connor V., and Karageorgos, Ioannis. Structure of the Fc fragment of the NIST reference antibody RM8671. United Kingdom: N. p., 2018. Web. doi:10.1107/S2053230X18009834.
Gallagher, D. Travis, Galvin, Connor V., & Karageorgos, Ioannis. Structure of the Fc fragment of the NIST reference antibody RM8671. United Kingdom. https://doi.org/10.1107/S2053230X18009834
Gallagher, D. Travis, Galvin, Connor V., and Karageorgos, Ioannis. Wed . "Structure of the Fc fragment of the NIST reference antibody RM8671". United Kingdom. https://doi.org/10.1107/S2053230X18009834.
@article{osti_1467531,
title = {Structure of the Fc fragment of the NIST reference antibody RM8671},
author = {Gallagher, D. Travis and Galvin, Connor V. and Karageorgos, Ioannis},
abstractNote = {As the link between antigen binding and immune activation, the antibody Fc region has received extensive structural study. In this report, the structure of the Fc fragment of the NIST IgG1 mAb (reference material 8671) is described at 2.1 Å resolution in space group P 2 1 2 1 2 1 , with approximate unit-cell parameters a  = 50, b = 80, c = 138 Å. Prior Fc structures with a wide variety of modifications are also surveyed, focusing on those in the same crystal form. To facilitate the analysis of conformations, a reference frame and a two-parameter metric are proposed, considering the CH2 domains as mobile with respect to a fixed dimeric CH3 core. Over several human Fc structures, a significant variation in Fc elbow conformations is observed, which may serve to facilitate the regulation of Fc effector signaling.},
doi = {10.1107/S2053230X18009834},
journal = {Acta Crystallographica. Section F, Structural Biology Communications},
number = 9,
volume = 74,
place = {United Kingdom},
year = {2018},
month = {8}
}

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