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Title: Native Mass Spectrometry Analysis of Oligomerization States of Fluorescence Recovery Protein and Orange Carotenoid Protein: Two Proteins Involved in the Cyanobacterial Photoprotection Cycle [Native Mass Spectrometry Analysis of Oligomerization States of FRP and OCP: Two Proteins Involved in the Cyanobacterial Photoprotection Cycle]

Abstract

Here, the orange carotenoid protein (OCP) and fluorescence recovery protein (FRP) are present in many cyanobacteria, and regulate an essential photoprotection cycle in an antagonistic manner as a function of light intensity. We characterized the oligomerization states of OCP and FRP by using native mass spectrometry, a technique that has the capability of studying native proteins under a wide range of protein concentrations and molecular masses. We found that dimeric FRP (dFRP) is the predominant state at protein concentrations ranging from 3 μM to 180 μM, and that higher order oligomers gradually form at protein concentrations above this range. The OCP, however, demonstrates significantly different oligomerization behavior. Monomeric OCP (mOCP) dominates at low protein concentrations, with an observable population of dimeric OCP (dOCP). The ratio of dOCP to mOCP, however, increases proportionally with the protein concentration. Higher order OCP oligomers form at protein concentrations beyond 10 μM. Additionally, native mass spectrometry coupled with ion mobility allowed us to measure protein collisional cross sections (CCS) and interrogate the unfolding of different FRP and OCP oligomers. We found that monomeric FRP exhibits a one-stage unfolding process, which could be correlated with its C-terminal bent crystal structure. The structural domain compositions of FRPmore » and OCP are compared and discussed.« less

Authors:
ORCiD logo [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1]; ORCiD logo [1];  [1]
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  1. Washington Univ. in St. Louis, St. Louis, MO (United States)
Publication Date:
Research Org.:
Washington Univ., St. Louis, MO (United States); Energy Frontier Research Centers (EFRC) (United States). Photosynthetic Antenna Research Center (PARC)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1465178
Grant/Contract Number:  
FG02-07ER15902; SC0001035
Resource Type:
Accepted Manuscript
Journal Name:
Biochemistry
Additional Journal Information:
Journal Volume: 56; Journal Issue: 1; Journal ID: ISSN 0006-2960
Publisher:
American Chemical Society (ACS)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Lu, Yue, Liu, Haijun, Saer, Rafael G., Zhang, Hao, Meyer, Christine M., Li, Veronica L., Shi, Liuqing, King, Jeremy D., Gross, Michael L., and Blankenship, Robert E. Native Mass Spectrometry Analysis of Oligomerization States of Fluorescence Recovery Protein and Orange Carotenoid Protein: Two Proteins Involved in the Cyanobacterial Photoprotection Cycle [Native Mass Spectrometry Analysis of Oligomerization States of FRP and OCP: Two Proteins Involved in the Cyanobacterial Photoprotection Cycle]. United States: N. p., 2016. Web. doi:10.1021/acs.biochem.6b01094.
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Lu, Yue, Liu, Haijun, Saer, Rafael G., Zhang, Hao, Meyer, Christine M., Li, Veronica L., Shi, Liuqing, King, Jeremy D., Gross, Michael L., & Blankenship, Robert E. Native Mass Spectrometry Analysis of Oligomerization States of Fluorescence Recovery Protein and Orange Carotenoid Protein: Two Proteins Involved in the Cyanobacterial Photoprotection Cycle [Native Mass Spectrometry Analysis of Oligomerization States of FRP and OCP: Two Proteins Involved in the Cyanobacterial Photoprotection Cycle]. United States. https://doi.org/10.1021/acs.biochem.6b01094
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Lu, Yue, Liu, Haijun, Saer, Rafael G., Zhang, Hao, Meyer, Christine M., Li, Veronica L., Shi, Liuqing, King, Jeremy D., Gross, Michael L., and Blankenship, Robert E. Thu . "Native Mass Spectrometry Analysis of Oligomerization States of Fluorescence Recovery Protein and Orange Carotenoid Protein: Two Proteins Involved in the Cyanobacterial Photoprotection Cycle [Native Mass Spectrometry Analysis of Oligomerization States of FRP and OCP: Two Proteins Involved in the Cyanobacterial Photoprotection Cycle]". United States. https://doi.org/10.1021/acs.biochem.6b01094. https://www.osti.gov/servlets/purl/1465178.
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@article{osti_1465178,
title = {Native Mass Spectrometry Analysis of Oligomerization States of Fluorescence Recovery Protein and Orange Carotenoid Protein: Two Proteins Involved in the Cyanobacterial Photoprotection Cycle [Native Mass Spectrometry Analysis of Oligomerization States of FRP and OCP: Two Proteins Involved in the Cyanobacterial Photoprotection Cycle]},
author = {Lu, Yue and Liu, Haijun and Saer, Rafael G. and Zhang, Hao and Meyer, Christine M. and Li, Veronica L. and Shi, Liuqing and King, Jeremy D. and Gross, Michael L. and Blankenship, Robert E.},
abstractNote = {Here, the orange carotenoid protein (OCP) and fluorescence recovery protein (FRP) are present in many cyanobacteria, and regulate an essential photoprotection cycle in an antagonistic manner as a function of light intensity. We characterized the oligomerization states of OCP and FRP by using native mass spectrometry, a technique that has the capability of studying native proteins under a wide range of protein concentrations and molecular masses. We found that dimeric FRP (dFRP) is the predominant state at protein concentrations ranging from 3 μM to 180 μM, and that higher order oligomers gradually form at protein concentrations above this range. The OCP, however, demonstrates significantly different oligomerization behavior. Monomeric OCP (mOCP) dominates at low protein concentrations, with an observable population of dimeric OCP (dOCP). The ratio of dOCP to mOCP, however, increases proportionally with the protein concentration. Higher order OCP oligomers form at protein concentrations beyond 10 μM. Additionally, native mass spectrometry coupled with ion mobility allowed us to measure protein collisional cross sections (CCS) and interrogate the unfolding of different FRP and OCP oligomers. We found that monomeric FRP exhibits a one-stage unfolding process, which could be correlated with its C-terminal bent crystal structure. The structural domain compositions of FRP and OCP are compared and discussed.},
doi = {10.1021/acs.biochem.6b01094},
journal = {Biochemistry},
number = 1,
volume = 56,
place = {United States},
year = {Thu Dec 08 00:00:00 EST 2016},
month = {Thu Dec 08 00:00:00 EST 2016}
}
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    Works referencing / citing this record:

    OCP–FRP protein complex topologies suggest a mechanism for controlling high light tolerance in cyanobacteria
    journal, September 2018

    • Sluchanko, Nikolai N.; Slonimskiy, Yury B.; Shirshin, Evgeny A.
    • Nature Communications, Vol. 9, Issue 1
    • DOI: 10.1038/s41467-018-06195-0

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    • Kerfeld, Cheryl A.; Melnicki, Matthew R.; Sutter, Markus
    • New Phytologist, Vol. 215, Issue 3
    • DOI: 10.1111/nph.14670

    OCP–FRP protein complex topologies suggest a mechanism for controlling high light tolerance in cyanobacteria
    text, January 2018

    • Sluchanko, Nikolai N.; Slonimskiy, Yury B.; Shirshin, Evgeny A.
    • Deutsches Elektronen-Synchrotron, DESY, Hamburg
    • DOI: 10.3204/pubdb-2018-05704

    Interdomain interactions reveal the molecular evolution of the orange carotenoid protein
    journal, September 2019

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