Structural analysis of a new carotenoid-binding protein: the C-terminal domain homolog of the OCP
Abstract
The Orange Carotenoid Protein (OCP) is a water-soluble protein that governs photoprotection in many cyanobacteria. The 35 kDa OCP is structurally and functionally modular, consisting of an N-terminal effector domain (NTD) and a C-terminal regulatory domain (CTD); a carotenoid spans the two domains. The CTD is a member of the ubiquitous Nuclear Transport Factor-2 (NTF2) superfamily (pfam02136). With the increasing availability of cyanobacterial genomes, bioinformatic analysis has revealed the existence of a new family of proteins, homologs to the CTD, the C-terminal domain-like carotenoid proteins (CCPs). Here we purify holo-CCP2 directly from cyanobacteria and establish that it natively binds canthaxanthin (CAN). We use small-angle X-ray scattering (SAXS) to characterize the structure of this carotenoprotein in two distinct oligomeric states. A single carotenoid molecule spans the two CCPs in the dimer. Our analysis with X-ray footprinting-mass spectrometry (XFMS) identifies critical residues for carotenoid binding that likely contribute to the extreme red shift (ca. 80 nm) of the absorption maximum of the carotenoid bound by the CCP2 dimer and a further 10 nm shift in the tetramer form. These data provide the first structural description of carotenoid binding by a protein consisting of only an NTF2 domain.
- Authors:
- Publication Date:
- Research Org.:
- Michigan State Univ., East Lansing, MI (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF); European Union (EU); Czech Science Foundation; National Institutes of Health (NIH)
- OSTI Identifier:
- 1664560
- Alternate Identifier(s):
- OSTI ID: 1711416; OSTI ID: 1713256
- Grant/Contract Number:
- SC0020606; AC02-05CH11231; FG02-91ER20021; IOS-1557324; 795070; 18-21631S; 1R01GM126218; P30-GM124169-01
- Resource Type:
- Published Article
- Journal Name:
- Scientific Reports
- Additional Journal Information:
- Journal Name: Scientific Reports Journal Volume: 10 Journal Issue: 1; Journal ID: ISSN 2045-2322
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United Kingdom
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; biochemistry; biophysical chemistry; proteins; structural biology
Citation Formats
Dominguez-Martin, Maria Agustina, Hammel, Michal, Gupta, Sayan, Lechno-Yossef, Sigal, Sutter, Markus, Rosenberg, Daniel J., Chen, Yan, Petzold, Christopher J., Ralston, Corie Y., Polívka, Tomáš, and Kerfeld, Cheryl A. Structural analysis of a new carotenoid-binding protein: the C-terminal domain homolog of the OCP. United Kingdom: N. p., 2020.
Web. doi:10.1038/s41598-020-72383-y.
Dominguez-Martin, Maria Agustina, Hammel, Michal, Gupta, Sayan, Lechno-Yossef, Sigal, Sutter, Markus, Rosenberg, Daniel J., Chen, Yan, Petzold, Christopher J., Ralston, Corie Y., Polívka, Tomáš, & Kerfeld, Cheryl A. Structural analysis of a new carotenoid-binding protein: the C-terminal domain homolog of the OCP. United Kingdom. https://doi.org/10.1038/s41598-020-72383-y
Dominguez-Martin, Maria Agustina, Hammel, Michal, Gupta, Sayan, Lechno-Yossef, Sigal, Sutter, Markus, Rosenberg, Daniel J., Chen, Yan, Petzold, Christopher J., Ralston, Corie Y., Polívka, Tomáš, and Kerfeld, Cheryl A. Wed .
"Structural analysis of a new carotenoid-binding protein: the C-terminal domain homolog of the OCP". United Kingdom. https://doi.org/10.1038/s41598-020-72383-y.
@article{osti_1664560,
title = {Structural analysis of a new carotenoid-binding protein: the C-terminal domain homolog of the OCP},
author = {Dominguez-Martin, Maria Agustina and Hammel, Michal and Gupta, Sayan and Lechno-Yossef, Sigal and Sutter, Markus and Rosenberg, Daniel J. and Chen, Yan and Petzold, Christopher J. and Ralston, Corie Y. and Polívka, Tomáš and Kerfeld, Cheryl A.},
abstractNote = {The Orange Carotenoid Protein (OCP) is a water-soluble protein that governs photoprotection in many cyanobacteria. The 35 kDa OCP is structurally and functionally modular, consisting of an N-terminal effector domain (NTD) and a C-terminal regulatory domain (CTD); a carotenoid spans the two domains. The CTD is a member of the ubiquitous Nuclear Transport Factor-2 (NTF2) superfamily (pfam02136). With the increasing availability of cyanobacterial genomes, bioinformatic analysis has revealed the existence of a new family of proteins, homologs to the CTD, the C-terminal domain-like carotenoid proteins (CCPs). Here we purify holo-CCP2 directly from cyanobacteria and establish that it natively binds canthaxanthin (CAN). We use small-angle X-ray scattering (SAXS) to characterize the structure of this carotenoprotein in two distinct oligomeric states. A single carotenoid molecule spans the two CCPs in the dimer. Our analysis with X-ray footprinting-mass spectrometry (XFMS) identifies critical residues for carotenoid binding that likely contribute to the extreme red shift (ca. 80 nm) of the absorption maximum of the carotenoid bound by the CCP2 dimer and a further 10 nm shift in the tetramer form. These data provide the first structural description of carotenoid binding by a protein consisting of only an NTF2 domain.},
doi = {10.1038/s41598-020-72383-y},
journal = {Scientific Reports},
number = 1,
volume = 10,
place = {United Kingdom},
year = {Wed Sep 23 00:00:00 EDT 2020},
month = {Wed Sep 23 00:00:00 EDT 2020}
}
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