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Title: Photosynthetic physiology and biomass partitioning in the model diatom Phaeodactylum tricornutum grown in a sinusoidal light regime

Abstract

Photosynthetic microbes respond to changing light environments to balance photosynthetic process with light induced damage and photoinhibition. There have been very few characterizations of photosynthetic physiology or biomass partitioning during the day in mass culture. Understanding the constraints on photosynthetic efficiency and biomass accumulation are necessary for engineering superior strains or cultivation methods. We observed the photosynthetic physiology of nutrient replete Phaeodactylum tricornutum growing in light environments that mimic those found in rapidly mixing, outdoor, low biomass photobioreactors. We found little evidence for photoinhibition or non-photochemical quenching in situ, suggesting photosynthesis remains highly efficient throughout the day. Cells doubled their organic carbon from dawn to dusk and a small percentage – around 20% – of this carbon was allocated to carbohydrates or triacylglycerol. We thus conclude that the self-shading provided by dense culturing of P. tricornutum inhibits the induction of photodamage, and energy dissipation processes that would otherwise lower productivity in an outdoor photobioreactor.

Authors:
 [1];  [1];  [1];  [1];  [1]
  1. Colorado State Univ., Fort Collins, CO (United States). Dept. of Biology
Publication Date:
Research Org.:
Colorado State Univ., Fort Collins, CO (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER); Bettencourt Schueller Foundation; National Science Foundation (NSF)
OSTI Identifier:
1435472
Alternate Identifier(s):
OSTI ID: 1438018
Grant/Contract Number:  
SC0008595
Resource Type:
Published Article
Journal Name:
Algal Research
Additional Journal Information:
Journal Name: Algal Research Journal Volume: 18 Journal Issue: C; Journal ID: ISSN 2211-9264
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; Phaeodactylum tricornutum; Cultivation; Biomass characterization; Photosynthetic physiology

Citation Formats

Jallet, Denis, Caballero, Michael A., Gallina, Alessandra A., Youngblood, Matthew, and Peers, Graham. Photosynthetic physiology and biomass partitioning in the model diatom Phaeodactylum tricornutum grown in a sinusoidal light regime. United States: N. p., 2016. Web. https://doi.org/10.1016/j.algal.2016.05.014.
Jallet, Denis, Caballero, Michael A., Gallina, Alessandra A., Youngblood, Matthew, & Peers, Graham. Photosynthetic physiology and biomass partitioning in the model diatom Phaeodactylum tricornutum grown in a sinusoidal light regime. United States. https://doi.org/10.1016/j.algal.2016.05.014
Jallet, Denis, Caballero, Michael A., Gallina, Alessandra A., Youngblood, Matthew, and Peers, Graham. Sat . "Photosynthetic physiology and biomass partitioning in the model diatom Phaeodactylum tricornutum grown in a sinusoidal light regime". United States. https://doi.org/10.1016/j.algal.2016.05.014.
@article{osti_1435472,
title = {Photosynthetic physiology and biomass partitioning in the model diatom Phaeodactylum tricornutum grown in a sinusoidal light regime},
author = {Jallet, Denis and Caballero, Michael A. and Gallina, Alessandra A. and Youngblood, Matthew and Peers, Graham},
abstractNote = {Photosynthetic microbes respond to changing light environments to balance photosynthetic process with light induced damage and photoinhibition. There have been very few characterizations of photosynthetic physiology or biomass partitioning during the day in mass culture. Understanding the constraints on photosynthetic efficiency and biomass accumulation are necessary for engineering superior strains or cultivation methods. We observed the photosynthetic physiology of nutrient replete Phaeodactylum tricornutum growing in light environments that mimic those found in rapidly mixing, outdoor, low biomass photobioreactors. We found little evidence for photoinhibition or non-photochemical quenching in situ, suggesting photosynthesis remains highly efficient throughout the day. Cells doubled their organic carbon from dawn to dusk and a small percentage – around 20% – of this carbon was allocated to carbohydrates or triacylglycerol. We thus conclude that the self-shading provided by dense culturing of P. tricornutum inhibits the induction of photodamage, and energy dissipation processes that would otherwise lower productivity in an outdoor photobioreactor.},
doi = {10.1016/j.algal.2016.05.014},
journal = {Algal Research},
number = C,
volume = 18,
place = {United States},
year = {2016},
month = {6}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1016/j.algal.2016.05.014

Citation Metrics:
Cited by: 10 works
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Works referenced in this record:

Influence of the Diadinoxanthin Pool Size on Photoprotection in the Marine Planktonic Diatom Phaeodactylum tricornutum
journal, June 2002

  • Lavaud, Johann; Rousseau, Bernard; van Gorkom, Hans J.
  • Plant Physiology, Vol. 129, Issue 3
  • DOI: 10.1104/pp.002014

Molecular mechanisms of photodamage in the Photosystem II complex
journal, January 2012


Diverse mechanisms for photoprotection in photosynthesis. Dynamic regulation of photosystem II excitation in response to rapid environmental change
journal, April 2015

  • Derks, Allen; Schaven, Kristin; Bruce, Doug
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1847, Issue 4-5
  • DOI: 10.1016/j.bbabio.2015.02.008

Genetic Engineering of Algae for Enhanced Biofuel Production
journal, February 2010

  • Radakovits, R.; Jinkerson, R. E.; Darzins, A.
  • Eukaryotic Cell, Vol. 9, Issue 4, p. 486-501
  • DOI: 10.1128/EC.00364-09

Biodiversity of NPQ
journal, January 2015


Measurements of variable chlorophyll fluorescence using fast repetition rate techniques: defining methodology and experimental protocols
journal, October 1998

  • Kolber, Zbigniew S.; Prášil, Ondřej; Falkowski, Paul G.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1367, Issue 1-3
  • DOI: 10.1016/S0005-2728(98)00135-2

Light and dark control of the cell cycle in two marine phytoplankton species
journal, November 1986


Microalgal bioreactors: Challenges and opportunities
journal, June 2009

  • Xu, Ling; Weathers, Pamela J.; Xiong, Xue-Rong
  • Engineering in Life Sciences, Vol. 9, Issue 3
  • DOI: 10.1002/elsc.200800111

Membrane Glycerolipid Remodeling Triggered by Nitrogen and Phosphorus Starvation in Phaeodactylum tricornutum
journal, December 2014

  • Abida, Heni; Dolch, Lina-Juana; Meï, Coline
  • Plant Physiology, Vol. 167, Issue 1
  • DOI: 10.1104/pp.114.252395

The super-excess energy dissipation in diatom algae: comparative analysis with higher plants
journal, November 2004

  • Ruban, Alexander; Lavaud, Johann; Rousseau, Bernard
  • Photosynthesis Research, Vol. 82, Issue 2
  • DOI: 10.1007/s11120-004-1456-1

In Search of a Physiological Basis for Covariations in Light-Limited and Light-Saturated Photosynthesis1: Photosynthetic Variability in Algae
journal, January 2004


Gene Regulation of Carbon Fixation, Storage, and Utilization in the Diatom Phaeodactylum tricornutum Acclimated to Light/Dark Cycles
journal, December 2012

  • Chauton, Matilde Skogen; Winge, Per; Brembu, Tore
  • Plant Physiology, Vol. 161, Issue 2
  • DOI: 10.1104/pp.112.206177

Regulatory branch points affecting protein and lipid biosynthesis in the diatom Phaeodactylum tricornutum
journal, December 2013


Auxiliary electron transport pathways in chloroplasts of microalgae
journal, July 2010

  • Peltier, Gilles; Tolleter, Dimitri; Billon, Emmanuelle
  • Photosynthesis Research, Vol. 106, Issue 1-2
  • DOI: 10.1007/s11120-010-9575-3

The amino acid and sugar composition of diatom cell-walls
journal, April 1973

  • Hecky, R. E.; Mopper, K.; Kilham, P.
  • Marine Biology, Vol. 19, Issue 4
  • DOI: 10.1007/BF00348902

Photosystem II Photoinactivation, Repair, and Protection in Marine Centric Diatoms
journal, July 2012

  • Wu, Hongyan; Roy, Suzanne; Alami, Meriem
  • Plant Physiology, Vol. 160, Issue 1
  • DOI: 10.1104/pp.112.203067

Lipids and lipid metabolism in eukaryotic algae
journal, March 2006


Photoprotection in the diatom Thalassiosira pseudonana: Role of LI818-like proteins in response to high light stress
journal, August 2010

  • Zhu, Song-Hua; Green, Beverley R.
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1797, Issue 8
  • DOI: 10.1016/j.bbabio.2010.04.003

The environmental photobioreactor (ePBR): An algal culturing platform for simulating dynamic natural environments
journal, October 2014


Photoinhibition of Photosynthesis in Nature
journal, June 1994


Photoinhibition of Photosystem II. Inactivation, protein damage and turnover
journal, July 1993

  • Aro, Eva-Mari; Virgin, Ivar; Andersson, Bertil
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1143, Issue 2
  • DOI: 10.1016/0005-2728(93)90134-2

Triacylglycerol accumulation and profiling in the model diatoms Thalassiosira pseudonana and Phaeodactylum tricornutum (Baccilariophyceae) during starvation
journal, January 2009

  • Yu, Eizadora T.; Zendejas, Frank J.; Lane, Pamela D.
  • Journal of Applied Phycology, Vol. 21, Issue 6
  • DOI: 10.1007/s10811-008-9400-y

Shear stress tolerance and biochemical characterization of Phaeodactylum tricornutum in quasi steady-state continuous culture in outdoor photobioreactors
journal, December 2003

  • Mirón, Asterio Sánchez; Garcı́a, M. Carmen Cerón; Gómez, Antonio Contreras
  • Biochemical Engineering Journal, Vol. 16, Issue 3
  • DOI: 10.1016/S1369-703X(03)00072-X

Rapid light curves: A powerful tool to assess photosynthetic activity
journal, July 2005


Singlet oxygen in plants: production, detoxification and signaling
journal, April 2009


Mixing in bubble columns: a new approach for characterizing dispersion coefficients
journal, October 2004

  • Camacho Rubio, F.; Sánchez Mirón, A.; Cerón García, M. C.
  • Chemical Engineering Science, Vol. 59, Issue 20
  • DOI: 10.1016/j.ces.2004.06.037

Genome-wide diel growth state transitions in the diatom Thalassiosira pseudonana
journal, April 2013

  • Ashworth, J.; Coesel, S.; Lee, A.
  • Proceedings of the National Academy of Sciences, Vol. 110, Issue 18
  • DOI: 10.1073/pnas.1300962110

Carbohydrate metabolism in the marine diatom Skeletonema costatum
journal, November 1969


Photoacclimation of Phaeodactylum tricornutum (Bacillariophyceae) cultures grown outdoors in photobioreactors and open ponds
journal, April 2012


Kinetics of light-intensity adaptation in a marine planktonic diatom
journal, January 1984

  • Post, A. F.; Dubinsky, Z.; Wyman, K.
  • Marine Biology, Vol. 83, Issue 3
  • DOI: 10.1007/BF00397454

New Fluorescence Parameters for the Determination of Q A Redox State and Excitation Energy Fluxes
journal, January 2004


The rate constant of photoinhibition, measured in lincomycin-treated leaves, is directly proportional to light intensity.
journal, March 1996

  • Tyystjarvi, E.; Aro, E. M.
  • Proceedings of the National Academy of Sciences, Vol. 93, Issue 5
  • DOI: 10.1073/pnas.93.5.2213

Energetic coupling between plastids and mitochondria drives CO2 assimilation in diatoms
journal, July 2015

  • Bailleul, Benjamin; Berne, Nicolas; Murik, Omer
  • Nature, Vol. 524, Issue 7565
  • DOI: 10.1038/nature14599

Balancing the energy flow from captured light to biomass under fluctuating light conditions
journal, January 2006


Diurnal rhythms in carbohydrate metabolism of the marine diatom Skeletonema costatum (Grev.) Cleve
journal, November 1986

  • Vårum, Kjell M.; Østgaard, Kjetill; Grimsrud, Kjersti
  • Journal of Experimental Marine Biology and Ecology, Vol. 102, Issue 2-3
  • DOI: 10.1016/0022-0981(86)90180-2

Remodeling of intermediate metabolism in the diatom Phaeodactylum tricornutum under nitrogen stress
journal, December 2014

  • Levitan, Orly; Dinamarca, Jorge; Zelzion, Ehud
  • Proceedings of the National Academy of Sciences, Vol. 112, Issue 2
  • DOI: 10.1073/pnas.1419818112

Singlet oxygen production in photosystem II and related protection mechanism
journal, September 2008

  • Krieger-Liszkay, Anja; Fufezan, Christian; Trebst, Achim
  • Photosynthesis Research, Vol. 98, Issue 1-3
  • DOI: 10.1007/s11120-008-9349-3

An atypical member of the light-harvesting complex stress-related protein family modulates diatom responses to light
journal, October 2010

  • Bailleul, B.; Rogato, A.; de Martino, A.
  • Proceedings of the National Academy of Sciences, Vol. 107, Issue 42
  • DOI: 10.1073/pnas.1007703107

A model for the relationship between light intensity and the rate of photosynthesis in phytoplankton
journal, September 1988


Light-regulated translation of chloroplast messenger RNAs through redox potential
journal, December 1994


Increasing algal photosynthetic productivity by integrating ecophysiology with systems biology
journal, November 2014


Triacylglycerol profiling of marine microalgae by mass spectrometry
journal, August 2011

  • Danielewicz, Megan A.; Anderson, Lisa A.; Franz, Annaliese K.
  • Journal of Lipid Research, Vol. 52, Issue 11
  • DOI: 10.1194/jlr.D018408

Manipulation of Photoprotection to Improve Plant Photosynthesis
journal, November 2010


Non-photochemical fluorescence quenching and the diadinoxanthin cycle in a marine diatom
journal, August 1994

  • Olaizola, Miguel; La Roche, Julie; Kolber, Zbigniew
  • Photosynthesis Research, Vol. 41, Issue 2
  • DOI: 10.1007/BF00019413

Distinctive Photosystem II Photoinactivation and Protein Dynamics in Marine Diatoms
journal, May 2011

  • Wu, Hongyan; Cockshutt, Amanda M.; McCarthy, Avery
  • Plant Physiology, Vol. 156, Issue 4
  • DOI: 10.1104/pp.111.178772

The regulation of xanthophyll cycle activity and of non-photochemical fluorescence quenching by two alternative electron flows in the diatoms Phaeodactylum tricornutum and Cyclotella meneghiniana
journal, July 2009

  • Grouneva, Irina; Jakob, Torsten; Wilhelm, Christian
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1787, Issue 7
  • DOI: 10.1016/j.bbabio.2009.02.004

Regulation of Photosynthesis during Abiotic Stress-Induced Photoinhibition
journal, September 2015


Light Dependence of Growth and Photosynthesis in Phaeodactylum Tricornutum (Bacillariophyceae)1
journal, December 1985


Photoprotection in plants: a new light on photosystem II damage
journal, January 2011


Safety valves for photosynthesis
journal, December 2000


    Works referencing / citing this record:

    Response of CO 2 -starved diatom Phaeodactylum tricornutum to light intensity transition
    journal, July 2017

    • Heydarizadeh, Parisa; Boureba, Wafâa; Zahedi, Morteza
    • Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 372, Issue 1728
    • DOI: 10.1098/rstb.2016.0396

    Effect of iron on the growth of Phaeodactylum tricornutum via photosynthesis
    journal, December 2017

    • Zhao, Peipei; Gu, Wenhui; Huang, Aiyou
    • Journal of Phycology, Vol. 54, Issue 1
    • DOI: 10.1111/jpy.12607

    Cross‐compartment metabolic coupling enables flexible photoprotective mechanisms in the diatom Phaeodactylum tricornutum
    journal, February 2019

    • Broddrick, Jared T.; Du, Niu; Smith, Sarah R.
    • New Phytologist, Vol. 222, Issue 3
    • DOI: 10.1111/nph.15685

    Dynamics of triacylglycerol and EPA production in Phaeodactylum tricornutum under nitrogen starvation at different light intensities
    journal, April 2017