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Title: Timing is everything: diel metabolic and physiological changes in the diatom Cyclotella cryptica grown in simulated outdoor conditions

Abstract

Microalgal cultures grown on a light-dark cycle experience diel patterns in metabolic and physiological processes, including cell cycle synchronization, but the implications for productivity in terms of biomass and commerciallyappealing molecules are not commonly appreciated. Despite a long history of diel response studies, only recently have photobioreactor technology advances enabled the use of sinusoidal light and temperature to more accurately mimic outdoor conditions. The present study investigates cell cycle progression and dynamic changes in biomass, triacylglycerol (TAG), and photosynthetic pigments on a 24-hour scale in the diatom Cyclotella cryptica grown using a sinusoidal light and temperature regime. Cell division synchronized to occur predominantly in the middle of the light period and biomass started to increase several hours earlier, as the cells prepared to divide. TAG levels increased during the day and decreased at night, with a mid-day dip corresponding to the time when lipid needs for cell divisionassociated membrane biosynthesis would be high. For the first time, photosynthetic pigment dynamics data, obtained with higher temporal resolution than previously reported for microalgae, was overlaid with cell cycle progression, indicating that while some photosynthetic pigments respond primarily to light, others are influenced by the cell cycle. Another novel observation was that themore » abundance of most photosynthetic pigments also fluctuated substantially on a rapid time scale during dawn, dusk, and the beginning of the dark period. Additionally, the results indicate that in a synchronized culture, potential product yields change substantially throughout the day. This may inform harvest timing to significantly increase yield.« less

Authors:
 [1];  [1];  [2];  [2];  [1];  [1]
  1. University of California, San Diego
  2. BATTELLE (PACIFIC NW LAB)
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1566285
Report Number(s):
PNNL-SA-138128
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Algal Research
Additional Journal Information:
Journal Volume: 42
Country of Publication:
United States
Language:
English
Subject:
microalgae, biofuels, Cell Lipids

Citation Formats

Gaidarenko, Olga, Sathoff, Corinne, Staub, Kenneth M., Huesemann, Michael H., Vernet, Maria, and Hildebrand, Mark. Timing is everything: diel metabolic and physiological changes in the diatom Cyclotella cryptica grown in simulated outdoor conditions. United States: N. p., 2019. Web. doi:10.1016/j.algal.2019.101598.
Gaidarenko, Olga, Sathoff, Corinne, Staub, Kenneth M., Huesemann, Michael H., Vernet, Maria, & Hildebrand, Mark. Timing is everything: diel metabolic and physiological changes in the diatom Cyclotella cryptica grown in simulated outdoor conditions. United States. doi:10.1016/j.algal.2019.101598.
Gaidarenko, Olga, Sathoff, Corinne, Staub, Kenneth M., Huesemann, Michael H., Vernet, Maria, and Hildebrand, Mark. Sun . "Timing is everything: diel metabolic and physiological changes in the diatom Cyclotella cryptica grown in simulated outdoor conditions". United States. doi:10.1016/j.algal.2019.101598.
@article{osti_1566285,
title = {Timing is everything: diel metabolic and physiological changes in the diatom Cyclotella cryptica grown in simulated outdoor conditions},
author = {Gaidarenko, Olga and Sathoff, Corinne and Staub, Kenneth M. and Huesemann, Michael H. and Vernet, Maria and Hildebrand, Mark},
abstractNote = {Microalgal cultures grown on a light-dark cycle experience diel patterns in metabolic and physiological processes, including cell cycle synchronization, but the implications for productivity in terms of biomass and commerciallyappealing molecules are not commonly appreciated. Despite a long history of diel response studies, only recently have photobioreactor technology advances enabled the use of sinusoidal light and temperature to more accurately mimic outdoor conditions. The present study investigates cell cycle progression and dynamic changes in biomass, triacylglycerol (TAG), and photosynthetic pigments on a 24-hour scale in the diatom Cyclotella cryptica grown using a sinusoidal light and temperature regime. Cell division synchronized to occur predominantly in the middle of the light period and biomass started to increase several hours earlier, as the cells prepared to divide. TAG levels increased during the day and decreased at night, with a mid-day dip corresponding to the time when lipid needs for cell divisionassociated membrane biosynthesis would be high. For the first time, photosynthetic pigment dynamics data, obtained with higher temporal resolution than previously reported for microalgae, was overlaid with cell cycle progression, indicating that while some photosynthetic pigments respond primarily to light, others are influenced by the cell cycle. Another novel observation was that the abundance of most photosynthetic pigments also fluctuated substantially on a rapid time scale during dawn, dusk, and the beginning of the dark period. Additionally, the results indicate that in a synchronized culture, potential product yields change substantially throughout the day. This may inform harvest timing to significantly increase yield.},
doi = {10.1016/j.algal.2019.101598},
journal = {Algal Research},
number = ,
volume = 42,
place = {United States},
year = {2019},
month = {9}
}