Robust biological nitrogen fixation in a model grass–bacterial association
Abstract
Summary Nitrogen‐fixing rhizobacteria can promote plant growth; however, it is controversial whether biological nitrogen fixation ( BNF ) from associative interaction contributes to growth promotion. The roots of Setaria viridis , a model C 4 grass, were effectively colonized by bacterial inoculants resulting in a significant enhancement of growth. Nitrogen‐13 tracer studies provided direct evidence for tracer uptake by the host plant and incorporation into protein. Indeed, plants showed robust growth under nitrogen‐limiting conditions when inoculated with an ammonium‐excreting strain of Azospirillum brasilense . 11 C‐labeling experiments showed that patterns in central carbon metabolism and resource allocation exhibited by nitrogen‐starved plants were largely reversed by bacterial inoculation, such that they resembled plants grown under nitrogen‐sufficient conditions. Adoption of S. viridis as a model should promote research into the mechanisms of associative nitrogen fixation with the ultimate goal of greater adoption of BNF for sustainable crop production.
- Authors:
-
- Department of Biochemistry and Molecular Biology Federal University of Paraná 81531‐980 Curitiba Brazil
- Department of Food Science and Technology Federal University of Santa Catarina 88034‐001 Florianópolis Brazil
- Department of Environmental Biology State University of New York College of Environmental Science and Forestry Syracuse NY 13210 USA
- Biological Environmental and Climate Sciences Department Brookhaven National Laboratory Building 901 Upton NY 11973 USA
- Divisions of Plant Science and Biochemistry C. S. Bond Life Science Center University of Missouri Columbia MO 65211 USA
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1401375
- Resource Type:
- Publisher's Accepted Manuscript
- Journal Name:
- The Plant Journal
- Additional Journal Information:
- Journal Name: The Plant Journal Journal Volume: 81 Journal Issue: 6; Journal ID: ISSN 0960-7412
- Publisher:
- Wiley-Blackwell
- Country of Publication:
- United Kingdom
- Language:
- English
Citation Formats
Pankievicz, Vânia C. S., do Amaral, Fernanda P., Santos, Karina F. D. N., Agtuca, Beverly, Xu, Youwen, Schueller, Michael J., Arisi, Ana Carolina M., Steffens, Maria. B. R., de Souza, Emanuel M., Pedrosa, Fábio O., Stacey, Gary, and Ferrieri, Richard A. Robust biological nitrogen fixation in a model grass–bacterial association. United Kingdom: N. p., 2015.
Web. doi:10.1111/tpj.12777.
Pankievicz, Vânia C. S., do Amaral, Fernanda P., Santos, Karina F. D. N., Agtuca, Beverly, Xu, Youwen, Schueller, Michael J., Arisi, Ana Carolina M., Steffens, Maria. B. R., de Souza, Emanuel M., Pedrosa, Fábio O., Stacey, Gary, & Ferrieri, Richard A. Robust biological nitrogen fixation in a model grass–bacterial association. United Kingdom. https://doi.org/10.1111/tpj.12777
Pankievicz, Vânia C. S., do Amaral, Fernanda P., Santos, Karina F. D. N., Agtuca, Beverly, Xu, Youwen, Schueller, Michael J., Arisi, Ana Carolina M., Steffens, Maria. B. R., de Souza, Emanuel M., Pedrosa, Fábio O., Stacey, Gary, and Ferrieri, Richard A. Tue .
"Robust biological nitrogen fixation in a model grass–bacterial association". United Kingdom. https://doi.org/10.1111/tpj.12777.
@article{osti_1401375,
title = {Robust biological nitrogen fixation in a model grass–bacterial association},
author = {Pankievicz, Vânia C. S. and do Amaral, Fernanda P. and Santos, Karina F. D. N. and Agtuca, Beverly and Xu, Youwen and Schueller, Michael J. and Arisi, Ana Carolina M. and Steffens, Maria. B. R. and de Souza, Emanuel M. and Pedrosa, Fábio O. and Stacey, Gary and Ferrieri, Richard A.},
abstractNote = {Summary Nitrogen‐fixing rhizobacteria can promote plant growth; however, it is controversial whether biological nitrogen fixation ( BNF ) from associative interaction contributes to growth promotion. The roots of Setaria viridis , a model C 4 grass, were effectively colonized by bacterial inoculants resulting in a significant enhancement of growth. Nitrogen‐13 tracer studies provided direct evidence for tracer uptake by the host plant and incorporation into protein. Indeed, plants showed robust growth under nitrogen‐limiting conditions when inoculated with an ammonium‐excreting strain of Azospirillum brasilense . 11 C‐labeling experiments showed that patterns in central carbon metabolism and resource allocation exhibited by nitrogen‐starved plants were largely reversed by bacterial inoculation, such that they resembled plants grown under nitrogen‐sufficient conditions. Adoption of S. viridis as a model should promote research into the mechanisms of associative nitrogen fixation with the ultimate goal of greater adoption of BNF for sustainable crop production.},
doi = {10.1111/tpj.12777},
journal = {The Plant Journal},
number = 6,
volume = 81,
place = {United Kingdom},
year = {Tue Mar 10 00:00:00 EDT 2015},
month = {Tue Mar 10 00:00:00 EDT 2015}
}
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