Metabolic associations with archaea drive shifts in hydrogen isotope fractionation in sulfate‐reducing bacterial lipids in cocultures and methane seeps
Abstract
Abstract Correlation between hydrogen isotope fractionation in fatty acids and carbon metabolism in pure cultures of bacteria indicates the potential of biomarker D/H analysis as a tool for diagnosing carbon substrate usage in environmental samples. However, most environments, in particular anaerobic habitats, are built from metabolic networks of micro‐organisms rather than a single organism. The effect of these networks on D/H of lipids has not been explored and may complicate the interpretation of these analyses. Syntrophy represents an extreme example of metabolic interdependence. Here, we analyzed the effect of metabolic interactions on the D/H biosignatures of sulfate‐reducing bacteria ( SRB ) using both laboratory maintained cocultures of the methanogen Methanosarcina acetivorans and the SRB Desulfococcus multivorans in addition to environmental samples harboring uncultured syntrophic consortia of anaerobic methane‐oxidizing archaea ( ANME ) and sulfate‐reducing Deltaproteobacteria ( SRB ) recovered from deep‐sea methane seeps. Consistent with previously reported trends, we observed a ~80‰ range in hydrogen isotope fractionation (ε lipid–water ) for D. multivorans grown under different carbon assimilation conditions, with more D‐enriched values associated with heterotrophic growth. In contrast, for cocultures of D. multivorans with M. acetivorans, we observed a reduced range of ε lipid – water values (~36‰) across substrates with shiftsmore »
- Authors:
-
- Division of Geological and Planetary Science California Institute of Technology Pasadena CA USA, Penn State Astrobiology Research Center Pennsylvania State University University Park PA USA
- Division of Geological and Planetary Science California Institute of Technology Pasadena CA USA, Department of Earth and Planetary Sciences Northwestern University Evanston IL USA
- Division of Geological and Planetary Science California Institute of Technology Pasadena CA USA
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1400922
- Resource Type:
- Publisher's Accepted Manuscript
- Journal Name:
- Geobiology
- Additional Journal Information:
- Journal Name: Geobiology Journal Volume: 13 Journal Issue: 5; Journal ID: ISSN 1472-4677
- Publisher:
- Wiley-Blackwell
- Country of Publication:
- United Kingdom
- Language:
- English
Citation Formats
Dawson, K. S., Osburn, M. R., Sessions, A. L., and Orphan, V. J. Metabolic associations with archaea drive shifts in hydrogen isotope fractionation in sulfate‐reducing bacterial lipids in cocultures and methane seeps. United Kingdom: N. p., 2015.
Web. doi:10.1111/gbi.12140.
Dawson, K. S., Osburn, M. R., Sessions, A. L., & Orphan, V. J. Metabolic associations with archaea drive shifts in hydrogen isotope fractionation in sulfate‐reducing bacterial lipids in cocultures and methane seeps. United Kingdom. https://doi.org/10.1111/gbi.12140
Dawson, K. S., Osburn, M. R., Sessions, A. L., and Orphan, V. J. Tue .
"Metabolic associations with archaea drive shifts in hydrogen isotope fractionation in sulfate‐reducing bacterial lipids in cocultures and methane seeps". United Kingdom. https://doi.org/10.1111/gbi.12140.
@article{osti_1400922,
title = {Metabolic associations with archaea drive shifts in hydrogen isotope fractionation in sulfate‐reducing bacterial lipids in cocultures and methane seeps},
author = {Dawson, K. S. and Osburn, M. R. and Sessions, A. L. and Orphan, V. J.},
abstractNote = {Abstract Correlation between hydrogen isotope fractionation in fatty acids and carbon metabolism in pure cultures of bacteria indicates the potential of biomarker D/H analysis as a tool for diagnosing carbon substrate usage in environmental samples. However, most environments, in particular anaerobic habitats, are built from metabolic networks of micro‐organisms rather than a single organism. The effect of these networks on D/H of lipids has not been explored and may complicate the interpretation of these analyses. Syntrophy represents an extreme example of metabolic interdependence. Here, we analyzed the effect of metabolic interactions on the D/H biosignatures of sulfate‐reducing bacteria ( SRB ) using both laboratory maintained cocultures of the methanogen Methanosarcina acetivorans and the SRB Desulfococcus multivorans in addition to environmental samples harboring uncultured syntrophic consortia of anaerobic methane‐oxidizing archaea ( ANME ) and sulfate‐reducing Deltaproteobacteria ( SRB ) recovered from deep‐sea methane seeps. Consistent with previously reported trends, we observed a ~80‰ range in hydrogen isotope fractionation (ε lipid–water ) for D. multivorans grown under different carbon assimilation conditions, with more D‐enriched values associated with heterotrophic growth. In contrast, for cocultures of D. multivorans with M. acetivorans, we observed a reduced range of ε lipid – water values (~36‰) across substrates with shifts of up to 61‰ compared to monocultures. Sediment cores from methane seep settings in Hydrate Ridge (offshore Oregon, USA ) showed similar D‐enrichment in diagnostic SRB fatty acids coinciding with peaks in ANME / SRB consortia concentration suggesting that metabolic associations are connected to the observed shifts in ε lipid–water values.},
doi = {10.1111/gbi.12140},
journal = {Geobiology},
number = 5,
volume = 13,
place = {United Kingdom},
year = {Tue Apr 28 00:00:00 EDT 2015},
month = {Tue Apr 28 00:00:00 EDT 2015}
}
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