Chlamydomonas as a model for biofuels and bio‐products production
Abstract
Summary Developing renewable energy sources is critical to maintaining the economic growth of the planet while protecting the environment. First generation biofuels focused on food crops like corn and sugarcane for ethanol production, and soybean and palm for biodiesel production. Second generation biofuels based on cellulosic ethanol produced from terrestrial plants, has received extensive funding and recently pilot facilities have been commissioned, but to date output of fuels from these sources has fallen well short of what is needed. Recent research and pilot demonstrations have highlighted the potential of algae as one of the most promising sources of sustainable liquid transportation fuels. Algae have also been established as unique biofactories for industrial, therapeutic, and nutraceutical co‐products. Chlamydomonas reinhardtii 's long established role in the field of basic research in green algae has paved the way for understanding algal metabolism and developing genetic engineering protocols. These tools are now being utilized in C. reinhardtii and in other algal species for the development of strains to maximize biofuels and bio‐products yields from the lab to the field.
- Authors:
-
- California Center for Algae Biotechnology Division of Biological Sciences University of California San Diego 9500 Gilman Dr La Jolla CA 92093‐0368 USA
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1400689
- Grant/Contract Number:
- DE‐EE0003373
- Resource Type:
- Publisher's Accepted Manuscript
- Journal Name:
- The Plant Journal
- Additional Journal Information:
- Journal Name: The Plant Journal Journal Volume: 82 Journal Issue: 3; Journal ID: ISSN 0960-7412
- Publisher:
- Wiley-Blackwell
- Country of Publication:
- United Kingdom
- Language:
- English
Citation Formats
Scranton, Melissa A., Ostrand, Joseph T., Fields, Francis J., and Mayfield, Stephen P. Chlamydomonas as a model for biofuels and bio‐products production. United Kingdom: N. p., 2015.
Web. doi:10.1111/tpj.12780.
Scranton, Melissa A., Ostrand, Joseph T., Fields, Francis J., & Mayfield, Stephen P. Chlamydomonas as a model for biofuels and bio‐products production. United Kingdom. https://doi.org/10.1111/tpj.12780
Scranton, Melissa A., Ostrand, Joseph T., Fields, Francis J., and Mayfield, Stephen P. Wed .
"Chlamydomonas as a model for biofuels and bio‐products production". United Kingdom. https://doi.org/10.1111/tpj.12780.
@article{osti_1400689,
title = {Chlamydomonas as a model for biofuels and bio‐products production},
author = {Scranton, Melissa A. and Ostrand, Joseph T. and Fields, Francis J. and Mayfield, Stephen P.},
abstractNote = {Summary Developing renewable energy sources is critical to maintaining the economic growth of the planet while protecting the environment. First generation biofuels focused on food crops like corn and sugarcane for ethanol production, and soybean and palm for biodiesel production. Second generation biofuels based on cellulosic ethanol produced from terrestrial plants, has received extensive funding and recently pilot facilities have been commissioned, but to date output of fuels from these sources has fallen well short of what is needed. Recent research and pilot demonstrations have highlighted the potential of algae as one of the most promising sources of sustainable liquid transportation fuels. Algae have also been established as unique biofactories for industrial, therapeutic, and nutraceutical co‐products. Chlamydomonas reinhardtii 's long established role in the field of basic research in green algae has paved the way for understanding algal metabolism and developing genetic engineering protocols. These tools are now being utilized in C. reinhardtii and in other algal species for the development of strains to maximize biofuels and bio‐products yields from the lab to the field.},
doi = {10.1111/tpj.12780},
journal = {The Plant Journal},
number = 3,
volume = 82,
place = {United Kingdom},
year = {Wed Feb 18 00:00:00 EST 2015},
month = {Wed Feb 18 00:00:00 EST 2015}
}
https://doi.org/10.1111/tpj.12780
Web of Science
Works referenced in this record:
Transgene and Transposon Silencing in Chlamydomonas reinhardtii by a DEAH-Box RNA Helicase
journal, November 2000
- Wu-Scharf, D.
- Science, Vol. 290, Issue 5494
High-Throughput Genotyping of Green Algal Mutants Reveals Random Distribution of Mutagenic Insertion Sites and Endonucleolytic Cleavage of Transforming DNA
journal, April 2014
- Zhang, Ru; Patena, Weronika; Armbruster, Ute
- The Plant Cell, Vol. 26, Issue 4
Molecular mechanisms of DNA double-strand break repair
journal, December 1998
- Kanaar, Roland; Hoeijmakers, Jan H. J.; van Gent, Dik C.
- Trends in Cell Biology, Vol. 8, Issue 12
Chlamydomonas starchless mutant defective in ADP-glucose pyrophosphorylase hyper-accumulates triacylglycerol
journal, July 2010
- Li, Yantao; Han, Danxiang; Hu, Guongrong
- Metabolic Engineering, Vol. 12, Issue 4, p. 387-391
Algal biofuels: Challenges and opportunities
journal, October 2013
- Leite, Gustavo B.; Abdelaziz, Ahmed E. M.; Hallenbeck, Patrick C.
- Bioresource Technology, Vol. 145
Production of therapeutic proteins in algae, analysis of expression of seven human proteins in the chloroplast of Chlamydomonas reinhardtii: Production of therapeutic proteins in algae
journal, March 2010
- Rasala, Beth A.; Muto, Machiko; Lee, Philip A.
- Plant Biotechnology Journal, Vol. 8, Issue 6
Chloroplast Biogenesis of Photosystem II Cores Involves a Series of Assembly-Controlled Steps That Regulate Translation
journal, December 2005
- Minai, Limor; Wostrikoff, Katia; Wollman, Francis-André
- The Plant Cell, Vol. 18, Issue 1
Homologous recombination in the nuclear genome of Chlamydomonas reinhardtii.
journal, October 1993
- Sodeinde, O. A.; Kindle, K. L.
- Proceedings of the National Academy of Sciences, Vol. 90, Issue 19
Microalgae for a macroenergy world
journal, October 2013
- Oncel, Suphi S.
- Renewable and Sustainable Energy Reviews, Vol. 26
Algae biofuels: versatility for the future of bioenergy
journal, June 2012
- Jones, Carla S.; Mayfield, Stephen P.
- Current Opinion in Biotechnology, Vol. 23, Issue 3
Technoeconomic Boundary Analysis of Biological Pathways to Hydrogen Production
report, September 2009
- James, B. D.; Baum, G. N.; Perez, J.
Alga-Produced Cholera Toxin-Pfs25 Fusion Proteins as Oral Vaccines
journal, April 2013
- Gregory, James A.; Topol, Aaron B.; Doerner, David Z.
- Applied and Environmental Microbiology, Vol. 79, Issue 13
The HSP70A promoter as a tool for the improved expression of transgenes in Chlamydomonas
journal, January 2000
- Schroda, Michael; Blocker, Dagmar; Beck, Christoph F.
- The Plant Journal, Vol. 21, Issue 2
Production of therapeutic proteins in the chloroplast of Chlamydomonas reinhardtii
journal, August 2014
- Almaraz-Delgado, Alma Lorena; Flores-Uribe, José; Pérez-España, Víctor Hugo
- AMB Express, Vol. 4, Issue 1
Overexpression of an exogenous phytoene synthase gene in the unicellular alga Chlamydomonas reinhardtii leads to an increase in the content of carotenoids
journal, January 2011
- Couso, I.; Vila, M.; Rodriguez, H.
- Biotechnology Progress, Vol. 27, Issue 1
A rapid live-cell ELISA for characterizing antibodies against cell surface antigens of Chlamydomonas reinhardtii and its use in isolating algae from natural environments with related cell wall components
journal, September 2014
- Jiang, Wenzhi; Cossey, Sarah; Rosenberg, Julian N.
- BMC Plant Biology, Vol. 14, Issue 1
Sustained Photobiological Hydrogen Gas Production upon Reversible Inactivation of Oxygen Evolution in the Green Alga Chlamydomonas reinhardtii
journal, January 2000
- Melis, Anastasios; Zhang, Liping; Forestier, Marc
- Plant Physiology, Vol. 122, Issue 1
Functional analysis of three type-2 DGAT homologue genes for triacylglycerol production in the green microalga Chlamydomonas reinhardtii
journal, November 2012
- La Russa, M.; Bogen, C.; Uhmeyer, A.
- Journal of Biotechnology, Vol. 162, Issue 1
Synthetic promoters genetic control through cis engineering
journal, March 2007
- Venter, Mauritz
- Trends in Plant Science, Vol. 12, Issue 3, p. 118-124
Fatty acid profiling of Chlamydomonas reinhardtii under nitrogen deprivation
journal, February 2011
- James, Gabriel O.; Hocart, Charles H.; Hillier, Warwick
- Bioresource Technology, Vol. 102, Issue 3
Hydrogen Production by Chlamydomonas reinhardtii
book, January 2013
- Torzillo, Giuseppe; Seibert, Michael
- Handbook of Microalgal Culture: Applied Phycology and Biotechnology, Second Edition
Production of anti-cancer immunotoxins in algae: Ribosome inactivating proteins as fusion partners: Production of Anti-Cancer Immunotoxins in Algae
journal, June 2013
- Tran, Miller; Henry, Ryan E.; Siefker, David
- Biotechnology and Bioengineering, Vol. 110, Issue 11
Hydrogen Fuel Production by Transgenic Microalgae
book, January 2007
- Melis, Anastasios; Seibert, Michael; Ghirardi, Maria L.
- Transgenic Microalgae as Green Cell Factories
Modularly assembled designer TAL effector nucleases for targeted gene knockout and gene replacement in eukaryotes
journal, March 2011
- Li, Ting; Huang, Sheng; Zhao, Xuefeng
- Nucleic Acids Research, Vol. 39, Issue 14
TALE activation of endogenous genes in Chlamydomonas reinhardtii
journal, July 2014
- Gao, Han; Wright, David A.; Li, Ting
- Algal Research, Vol. 5
Improvement of hydrogen yield of lba-transgenic Chlamydomonas reinhardtii caused by increasing respiration and impairing photosynthesis
journal, August 2014
- Xu, Lili; Wang, Quanxi; Wu, Shuangxiu
- International Journal of Hydrogen Energy, Vol. 39, Issue 25
Enhancement of carotenoids biosynthesis in Chlamydomonas reinhardtii by nuclear transformation using a phytoene synthase gene isolated from Chlorella zofingiensis
journal, April 2011
- Cordero, Baldo F.; Couso, Inmaculada; León, Rosa
- Applied Microbiology and Biotechnology, Vol. 91, Issue 2
An inducible artificial microRNA system for Chlamydomonas reinhardtii confirms a key role for heat shock factor 1 in regulating thermotolerance
journal, May 2010
- Schmollinger, Stefan; Strenkert, Daniela; Schroda, Michael
- Current Genetics, Vol. 56, Issue 4
Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast
journal, May 2007
- Manuell, Andrea L.; Beligni, Maria Verónica; Elder, John H.
- Plant Biotechnology Journal, Vol. 5, Issue 3
An updated comprehensive techno-economic analysis of algae biodiesel
journal, October 2013
- Nagarajan, Sanjay; Chou, Siaw Kiang; Cao, Shenyan
- Bioresource Technology, Vol. 145
Artificial control of transgene expression in Chlamydomonas reinhardtii chloroplast using the lac regulation system from Escherichia coli
journal, September 2007
- Kato, Ko; Marui, Takahiro; Kasai, Seitaro
- Journal of Bioscience and Bioengineering, Vol. 104, Issue 3
Importance of algae oil as a source of biodiesel
journal, January 2011
- Demirbas, Ayhan; Fatih Demirbas, M.
- Energy Conversion and Management, Vol. 52, Issue 1
Photobioreactors for mass cultivation of algae
journal, July 2008
- Ugwu, C. U.; Aoyagi, H.; Uchiyama, H.
- Bioresource Technology, Vol. 99, Issue 10, p. 4021-4028
Increased Lipid Accumulation in the Chlamydomonas reinhardtii sta7-10 Starchless Isoamylase Mutant and Increased Carbohydrate Synthesis in Complemented Strains
journal, June 2010
- Work, Victoria H.; Radakovits, Randor; Jinkerson, Robert E.
- Eukaryotic Cell, Vol. 9, Issue 8
Transgenic microalgae expressing Escherichia coli AppA phytase as feed additive to reduce phytate excretion in the manure of young broiler chicks
journal, April 2011
- Yoon, So-Mi; Kim, So Young; Li, Kun Feng
- Applied Microbiology and Biotechnology, Vol. 91, Issue 3
Algal biodiversity
journal, July 1996
- Norton, Trevor A.; Melkonian, Michael; Andersen, Robert A.
- Phycologia, Vol. 35, Issue 4
Recombination and Heterologous Expression of Allophycocyanin Gene in the Chloroplast of Chlamydomonas reinhardtii
journal, October 2005
- Su, Zhong-Liang; Qian, Kai-Xian; Tan, Cong-Ping
- Acta Biochimica et Biophysica Sinica, Vol. 37, Issue 10
Photosynthetic biomanufacturing in green algae; production of recombinant proteins for industrial, nutritional, and medical uses
journal, March 2014
- Rasala, Beth A.; Mayfield, Stephen P.
- Photosynthesis Research, Vol. 123, Issue 3
System and method for research-scale outdoor production of microalgae and cyanobacteria
journal, August 2014
- Schoepp, Nathan G.; Stewart, Ryan L.; Sun, Vincent
- Bioresource Technology, Vol. 166
The future viability of algae-derived biodiesel under economic and technical uncertainties
journal, January 2014
- Brownbridge, George; Azadi, Pooya; Smallbone, Andrew
- Bioresource Technology, Vol. 151
TAG, You’re it! Chlamydomonas as a reference organism for understanding algal triacylglycerol accumulation
journal, June 2012
- Merchant, Sabeeha S.; Kropat, Janette; Liu, Bensheng
- Current Opinion in Biotechnology, Vol. 23, Issue 3
Metabolic and gene expression changes triggered by nitrogen deprivation in the photoautotrophically grown microalgae Chlamydomonas reinhardtii and Coccomyxa sp. C-169
journal, March 2012
- Msanne, Joseph; Xu, Di; Konda, Anji Reddy
- Phytochemistry, Vol. 75
Increased hydrogen photoproduction by means of a sulfur-deprived Chlamydomonas reinhardtii D1 protein mutant
journal, May 2009
- Torzillo, Giuseppe; Scoma, Alberto; Faraloni, Cecilia
- International Journal of Hydrogen Energy, Vol. 34, Issue 10
Biopharmaceutical benchmarks 2014
journal, October 2014
- Walsh, Gary
- Nature Biotechnology, Vol. 32, Issue 10
The Roles of acyl-CoA: Diacylglycerol Acyltransferase 2 Genes in the Biosynthesis of Triacylglycerols by the Green Algae Chlamydomonas reinhardtii
journal, July 2012
- Deng, Xiao-Dong; Gu, Bo; Li, Ya-Jun
- Molecular Plant, Vol. 5, Issue 4
Deletion of the Pichia pastoris KU70 Homologue Facilitates Platform Strain Generation for Gene Expression and Synthetic Biology
journal, June 2012
- Näätsaari, Laura; Mistlberger, Beate; Ruth, Claudia
- PLoS ONE, Vol. 7, Issue 6
The Conserved TFLK Motif of Mammary-Associated Serum Amyloid A3 Is Responsible for Up-regulation of Intestinal MUC3 Mucin Expression In Vitro
journal, January 2003
- Mack, David R.; McDonald, Thomas L.; Larson, Marilynn A.
- Pediatric Research, Vol. 53, Issue 1
RNA Interference Silencing of a Major Lipid Droplet Protein Affects Lipid Droplet Size in Chlamydomonas reinhardtii
journal, November 2009
- Moellering, Eric R.; Benning, Christoph
- Eukaryotic Cell, Vol. 9, Issue 1
Heat-stable oral alga-based vaccine protects mice from Staphylococcus aureus infection
journal, February 2010
- Dreesen, Imke A. J.; Hamri, Ghislaine Charpin-El; Fussenegger, Martin
- Journal of Biotechnology, Vol. 145, Issue 3
Highly specific gene silencing by artificial microRNAs in the unicellular alga Chlamydomonas reinhardtii: Artificial miRNAs in Chlamydomonas
journal, January 2009
- Molnar, Attila; Bassett, Andrew; Thuenemann, Eva
- The Plant Journal, Vol. 58, Issue 1
Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice
journal, August 2013
- Jiang, Wenzhi; Zhou, Huanbin; Bi, Honghao
- Nucleic Acids Research, Vol. 41, Issue 20
Biohydrogen production using mutant strains of Chlamydomonas reinhardtii: The effects of light intensity and illumination patterns
journal, November 2014
- Oncel, S. S.; Kose, A.; Faraloni, C.
- Biochemical Engineering Journal, Vol. 92
Production of unique immunotoxin cancer therapeutics in algal chloroplasts
journal, December 2012
- Tran, M.; Van, C.; Barrera, D. J.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 1
The Path to Triacylglyceride Obesity in the sta6 Strain of Chlamydomonas reinhardtii
journal, February 2014
- Goodenough, Ursula; Blaby, Ian; Casero, David
- Eukaryotic Cell, Vol. 13, Issue 5
Edible oils from microalgae: insights in TAG accumulation
journal, October 2014
- Klok, A. J.; Lamers, P. P.; Martens, D. E.
- Trends in Biotechnology, Vol. 32, Issue 10
Synthetic Oligonucleotide Libraries Reveal Novel Regulatory Elements in Chlamydomonas Chloroplast mRNAs
journal, September 2012
- Specht, Elizabeth A.; Mayfield, Stephen P.
- ACS Synthetic Biology, Vol. 2, Issue 1
Outdoor cultivation of microalgae for carotenoid production: current state and perspectives
journal, February 2007
- Del Campo, José A.; García-González, Mercedes; Guerrero, Miguel G.
- Applied Microbiology and Biotechnology, Vol. 74, Issue 6
Production of recombinant enzymes in the marine alga Dunaliella tertiolecta
journal, January 2013
- Georgianna, D. Ryan; Hannon, Michael J.; Marcuschi, Marina
- Algal Research, Vol. 2, Issue 1
Manipulating Fatty Acid Biosynthesis in Microalgae for Biofuel through Protein-Protein Interactions
journal, September 2012
- Blatti, Jillian L.; Beld, Joris; Behnke, Craig A.
- PLoS ONE, Vol. 7, Issue 9
Strategies to facilitate transgene expression in Chlamydomonas reinhardtii
journal, January 2009
- Eichler-Stahlberg, Alke; Weisheit, Wolfram; Ruecker, Ovidiu
- Planta, Vol. 229, Issue 4
Three Acyltransferases and Nitrogen-responsive Regulator Are Implicated in Nitrogen Starvation-induced Triacylglycerol Accumulation in Chlamydomonas
journal, March 2012
- Boyle, Nanette R.; Page, Mark Dudley; Liu, Bensheng
- Journal of Biological Chemistry, Vol. 287, Issue 19
Strategies for psbA gene expression in cyanobacteria, green algae and higher plants: From transcription to PSII repair
journal, January 2012
- Mulo, Paula; Sakurai, Isamu; Aro, Eva-Mari
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1817, Issue 1
Decoding algal genomes: tracing back the history of photosynthetic life on Earth: Decoding algal genomes
journal, March 2011
- Tirichine, Leïla; Bowler, Chris
- The Plant Journal, Vol. 66, Issue 1
Aquatic phototrophs: efficient alternatives to land-based crops for biofuels
journal, June 2008
- Dismukes, G. Charles; Carrieri, Damian; Bennette, Nicholas
- Current Opinion in Biotechnology, Vol. 19, Issue 3
Mechanism and challenges in commercialisation of algal biofuels
journal, January 2011
- Singh, Anoop; Nigam, Poonam Singh; Murphy, Jerry D.
- Bioresource Technology, Vol. 102, Issue 1
High-throughput fluorescence-activated cell sorting for lipid hyperaccumulating Chlamydomonas reinhardtii mutants
journal, April 2014
- Xie, Bo; Stessman, Dan; Hart, Jason H.
- Plant Biotechnology Journal, Vol. 12, Issue 7
Genetic Engineering to Improve Algal Biofuels Production
book, November 2012
- Rasala, Beth A.; Gimpel, Javier A.; Tran, Miller
- Algae for Biofuels and Energy
Outdoor H2 production in a 50-L tubular photobioreactor by means of a sulfur-deprived culture of the microalga Chlamydomonas reinhardtii
journal, February 2012
- Scoma, Alberto; Giannelli, Luca; Faraloni, Cecilia
- Journal of Biotechnology, Vol. 157, Issue 4
Studies on homologous recombination in the green alga Chlamydomonas reinhardtii
journal, January 1994
- Gumpel, Nicola J.; Rochaix, Jean-David; Purton, Saul
- Current Genetics, Vol. 26, Issue 5-6
Identification and microbial production of a terpene-based advanced biofuel
journal, September 2011
- Peralta-Yahya, Pamela P.; Ouellet, Mario; Chan, Rossana
- Nature Communications, Vol. 2, Issue 1
Oil accumulation in the model green alga Chlamydomonas reinhardtii: characterization, variability between common laboratory strains and relationship with starch reserves
journal, January 2011
- Siaut, Magali; Cuiné, Stéphan; Cagnon, Caroline
- BMC Biotechnology, Vol. 11, Issue 1
Plastidial Expression of Type II NAD(P)H Dehydrogenase Increases the Reducing State of Plastoquinones and Hydrogen Photoproduction Rate by the Indirect Pathway in Chlamydomonas reinhardtii
journal, May 2014
- Baltz, Anthony; Dang, Kieu-Van; Beyly, Audrey
- Plant Physiology, Vol. 165, Issue 3
Efficient H2 production via Chlamydomonas reinhardtii
journal, December 2011
- Esquível, Maria G.; Amaro, Helena M.; Pinto, Teresa S.
- Trends in Biotechnology, Vol. 29, Issue 12
Successful Transient Expression of Cas9 and Single Guide RNA Genes in Chlamydomonas reinhardtii
journal, September 2014
- Jiang, Wenzhi; Brueggeman, Andrew J.; Horken, Kempton M.
- Eukaryotic Cell, Vol. 13, Issue 11
Structural Correlates of Cytoplasmic and Chloroplast Lipid Body Synthesis in Chlamydomonas reinhardtii and Stimulation of Lipid Body Production with Acetate Boost
journal, October 2011
- Goodson, Carrie; Roth, Robyn; Wang, Zi Teng
- Eukaryotic Cell, Vol. 10, Issue 12
Selenoprotein-Transgenic Chlamydomonas reinhardtii
journal, February 2013
- Hou, Qintang; Qiu, Shi; Liu, Qiong
- Nutrients, Vol. 5, Issue 3
Exploiting diversity and synthetic biology for the production of algal biofuels
journal, August 2012
- Georgianna, D. Ryan; Mayfield, Stephen P.
- Nature, Vol. 488, Issue 7411
Lipid productivity as a key characteristic for choosing algal species for biodiesel production
journal, January 2009
- Griffiths, Melinda J.; Harrison, Susan T. L.
- Journal of Applied Phycology, Vol. 21, Issue 5
Targeted disruption of the NIT8 gene in Chlamydomonas reinhardtii.
journal, October 1995
- Nelson, J. A.; Lefebvre, P. A.
- Molecular and Cellular Biology, Vol. 15, Issue 10
Genomic Insights into Marine Microalgae
journal, December 2008
- Parker, Micaela S.; Mock, Thomas; Armbrust, E. Virginia
- Annual Review of Genetics, Vol. 42, Issue 1
Hydrogenases and Hydrogen Photoproduction in Oxygenic Photosynthetic Organisms
journal, June 2007
- Ghirardi, Maria L.; Posewitz, Matthew C.; Maness, Pin-Ching
- Annual Review of Plant Biology, Vol. 58, Issue 1
Assembly of the Light-Harvesting Chlorophyll Antenna in the Green Alga Chlamydomonas reinhardtii Requires Expression of the TLA2 - CpFTSY Gene
journal, November 2011
- Kirst, Henning; García-Cerdán, Jose Gines; Zurbriggen, Andreas
- Plant Physiology, Vol. 158, Issue 2
The microalga Chlamydomonas reinhardtii as a platform for the production of human protein therapeutics
journal, January 2011
- Rasala, Beth A.; Mayfield, Stephen P.
- Bioengineered Bugs, Vol. 2, Issue 1
Gene silencing by artificial microRNAs in Chlamydomonas: Gene silencing by artificial miRNAs in Chlamydomonas
journal, December 2008
- Zhao, Tao; Wang, Wei; Bai, Xue
- The Plant Journal, Vol. 58, Issue 1
Survival of Human Metallothionein-2 Transplastomic Chlamydomonas reinhardtii to Ultraviolet B Exposure
journal, March 2006
- Zhang, Yu-Kun; Shen, Gui-Fang; Ru, Bing-Gen
- Acta Biochimica et Biophysica Sinica, Vol. 38, Issue 3
Engineered Photosystem II Reaction Centers Optimize Photochemistry versus Photoprotection at Different Solar Intensities
journal, February 2014
- Vinyard, David J.; Gimpel, Javier; Ananyev, Gennady M.
- Journal of the American Chemical Society, Vol. 136, Issue 10
Natural Variants of Photosystem II Subunit D1 Tune Photochemical Fitness to Solar Intensity
journal, December 2012
- Vinyard, David J.; Gimpel, Javier; Ananyev, Gennady M.
- Journal of Biological Chemistry, Vol. 288, Issue 8
Stable Integration and Functional Expression of Flounder Growth Hormone Gene in Transformed Microalga, Chlorella ellipsoidea
journal, January 2002
- Kim, Dae-Hyun; Kim, Young Tae; Cho, Jung Jong
- Marine Biotechnology, Vol. 4, Issue 1
Human serum amyloid A3 peptide enhances intestinal MUC3 expression and inhibits EPEC adherence
journal, January 2003
- Larson, Marilynn A.; Wei, Shu H.; Weber, Annika
- Biochemical and Biophysical Research Communications, Vol. 300, Issue 2
Expression of human soluble TRAIL in Chlamydomonas reinhardtii chloroplast
journal, July 2006
- Yang, Zongqi; Li, yinü; Chen, Feng
- Chinese Science Bulletin, Vol. 51, Issue 14
Algal Lipid Bodies: Stress Induction, Purification, and Biochemical Characterization in Wild-Type and Starchless Chlamydomonas reinhardtii
journal, October 2009
- Wang, Zi Teng; Ullrich, Nico; Joo, Sunjoo
- Eukaryotic Cell, Vol. 8, Issue 12
Unravelling the genomic mosaic of a ubiquitous genus of marine cyanobacteria
journal, January 2008
- Dufresne, Alexis; Ostrowski, Martin; Scanlan, David J.
- Genome Biology, Vol. 9, Issue 5
Chloroplast transformation in Chlamydomonas with high velocity microprojectiles
journal, June 1988
- Boynton, J.; Gillham, N.; Harris, E.
- Science, Vol. 240, Issue 4858
Chlamydomonas reinhardtii as a viable platform for the production of recombinant proteins: current status and perspectives
journal, November 2011
- Rosales-Mendoza, Sergio; Paz-Maldonado, Luz María Teresita; Soria-Guerra, Ruth Elena
- Plant Cell Reports, Vol. 31, Issue 3
Expanding the spectral palette of fluorescent proteins for the green microalga Chlamydomonas reinhardtii
journal, April 2013
- Rasala, Beth A.; Barrera, Daniel J.; Ng, Jenny
- The Plant Journal, Vol. 74, Issue 4
A fluorescence-activated cell sorting-based strategy for rapid isolation of high-lipid Chlamydomonas mutants
journal, October 2014
- Terashima, Mia; Freeman, Elizabeth S.; Jinkerson, Robert E.
- The Plant Journal, Vol. 81, Issue 1
Epigenetic silencing of a foreign gene in nuclear transformants of Chlamydomonas.
journal, June 1997
- Cerutti, H.; Johnson, A. M.; Gillham, N. W.
- The Plant Cell, Vol. 9, Issue 6
Robust Expression and Secretion of Xylanase1 in Chlamydomonas reinhardtii by Fusion to a Selection Gene and Processing with the FMDV 2A Peptide
journal, August 2012
- Rasala, Beth A.; Lee, Philip A.; Shen, Zhouxin
- PLoS ONE, Vol. 7, Issue 8
Production of recombinant proteins in microalgae at pilot greenhouse scale: Recombinant Protein Production in Algae
journal, September 2014
- Gimpel, Javier A.; Hyun, James S.; Schoepp, Nathan G.
- Biotechnology and Bioengineering, Vol. 112, Issue 2