Characterization of novel Brown midrib 6 mutations affecting lignin biosynthesis in sorghum
Abstract
Abstract The presence of lignin reduces the quality of lignocellulosic biomass for forage materials and feedstock for biofuels. In C4 grasses, the brown midrib phenotype has been linked to mutations to genes in the monolignol biosynthesis pathway. For example, the Bmr6 gene in sorghum ( Sorghum bicolor ) has been previously shown to encode cinnamyl alcohol dehydrogenase (CAD), which catalyzes the final step of the monolignol biosynthesis pathway. Mutations in this gene have been shown to reduce the abundance of lignin, enhance digestibility, and improve saccharification efficiencies and ethanol yields. Nine sorghum lines harboring five different bmr6 alleles were identified in an EMS‐mutagenized TILLING population. DNA sequencing of Bmr6 revealed that the majority of the mutations impacted evolutionarily conserved amino acids while three‐dimensional structural modeling predicted that all of these alleles interfered with the enzyme's ability to bind with its NADPH cofactor. All of the new alleles reduced in vitro CAD activity levels and enhanced glucose yields following saccharification. Further, many of these lines were associated with higher reductions in acid detergent lignin compared to lines harboring the previously characterized bmr6‐ref allele. These bmr6 lines represent new breeding tools for manipulating biomass composition to enhance forage and feedstock quality.
- Authors:
-
- Grain, Forage, and Bioenergy Research Unit USDA‐ARS, Lincoln NE 68583 USA, Department of Agronomy and Horticulture University of Nebraska‐Lincoln Lincoln NE 68583 USA
- Grain, Forage, and Bioenergy Research Unit USDA‐ARS, Lincoln NE 68583 USA
- Grain, Forage, and Bioenergy Research Unit USDA‐ARS, Lincoln NE 68583 USA, Department of Plant Pathology University of Nebraska‐Lincoln Lincoln NE 68583 USA
- Plant Stress and Germplasm Development Unit USDA‐ARS Lubbock TX 79414 USA
- Department of Chemistry University of Nebraska‐Kearney Kearney NE 68849 USA
- Department of Microbiology &, Cell Science and UF Genetics Institute University of Florida Gainesville FL 32610 USA
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1400522
- Grant/Contract Number:
- DE‐FG02‐07ER64458
- Resource Type:
- Publisher's Accepted Manuscript
- Journal Name:
- Journal of Integrative Plant Biology
- Additional Journal Information:
- Journal Name: Journal of Integrative Plant Biology Journal Volume: 58 Journal Issue: 2; Journal ID: ISSN 1672-9072
- Publisher:
- Wiley-Blackwell
- Country of Publication:
- United Kingdom
- Language:
- English
Citation Formats
Scully, Erin D., Gries, Tammy, Funnell‐Harris, Deanna L., Xin, Zhanguo, Kovacs, Frank A., Vermerris, Wilfred, and Sattler, Scott E. Characterization of novel Brown midrib 6 mutations affecting lignin biosynthesis in sorghum. United Kingdom: N. p., 2015.
Web. doi:10.1111/jipb.12375.
Scully, Erin D., Gries, Tammy, Funnell‐Harris, Deanna L., Xin, Zhanguo, Kovacs, Frank A., Vermerris, Wilfred, & Sattler, Scott E. Characterization of novel Brown midrib 6 mutations affecting lignin biosynthesis in sorghum. United Kingdom. https://doi.org/10.1111/jipb.12375
Scully, Erin D., Gries, Tammy, Funnell‐Harris, Deanna L., Xin, Zhanguo, Kovacs, Frank A., Vermerris, Wilfred, and Sattler, Scott E. Fri .
"Characterization of novel Brown midrib 6 mutations affecting lignin biosynthesis in sorghum". United Kingdom. https://doi.org/10.1111/jipb.12375.
@article{osti_1400522,
title = {Characterization of novel Brown midrib 6 mutations affecting lignin biosynthesis in sorghum},
author = {Scully, Erin D. and Gries, Tammy and Funnell‐Harris, Deanna L. and Xin, Zhanguo and Kovacs, Frank A. and Vermerris, Wilfred and Sattler, Scott E.},
abstractNote = {Abstract The presence of lignin reduces the quality of lignocellulosic biomass for forage materials and feedstock for biofuels. In C4 grasses, the brown midrib phenotype has been linked to mutations to genes in the monolignol biosynthesis pathway. For example, the Bmr6 gene in sorghum ( Sorghum bicolor ) has been previously shown to encode cinnamyl alcohol dehydrogenase (CAD), which catalyzes the final step of the monolignol biosynthesis pathway. Mutations in this gene have been shown to reduce the abundance of lignin, enhance digestibility, and improve saccharification efficiencies and ethanol yields. Nine sorghum lines harboring five different bmr6 alleles were identified in an EMS‐mutagenized TILLING population. DNA sequencing of Bmr6 revealed that the majority of the mutations impacted evolutionarily conserved amino acids while three‐dimensional structural modeling predicted that all of these alleles interfered with the enzyme's ability to bind with its NADPH cofactor. All of the new alleles reduced in vitro CAD activity levels and enhanced glucose yields following saccharification. Further, many of these lines were associated with higher reductions in acid detergent lignin compared to lines harboring the previously characterized bmr6‐ref allele. These bmr6 lines represent new breeding tools for manipulating biomass composition to enhance forage and feedstock quality.},
doi = {10.1111/jipb.12375},
journal = {Journal of Integrative Plant Biology},
number = 2,
volume = 58,
place = {United Kingdom},
year = {Fri Oct 09 00:00:00 EDT 2015},
month = {Fri Oct 09 00:00:00 EDT 2015}
}
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