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Title: Degradation of lignin β-aryl ether units in Arabidopsis thaliana expressing LigD , LigF and LigG from Sphingomonas paucimobilis SYK-6

Here, lignin is a major polymer in the secondary plant cell wall and composed of hydrophobic interlinked hydroxyphenylpropanoid units. The presence of lignin hampers conversion of plant biomass into biofuels; plants with modified lignin are therefore being investigated for increased digestibility. The bacterium Sphingomonas paucimobilis produces lignin-degrading enzymes including LigD, LigF and LigG involved in cleaving the most abundant lignin interunit linkage, the β-aryl ether bond. In this study, we expressed the LigD, LigF and LigG ( LigDFG) genes in Arabidopsis thaliana to introduce postlignification modifications into the lignin structure. The three enzymes were targeted to the secretory pathway. Phenolic metabolite profiling and 2D HSQC NMR of the transgenic lines showed an increase in oxidized guaiacyl and syringyl units without concomitant increase in oxidized β-aryl ether units, showing lignin bond cleavage. Saccharification yield increased significantly in transgenic lines expressing LigDFG, showing the applicability of our approach. Additional new information on substrate specificity of the LigDFG enzymes is also provided.
Authors:
 [1] ;  [2] ;  [2] ;  [3] ;  [3] ;  [4] ;  [1] ;  [1] ;  [2] ;  [3] ;  [1] ;  [5] ;  [1] ;  [5]
  1. Univ. of Copenhagen, Frederiksberg C (Denmark)
  2. Ghent Univ., Ghent (Belgium); VIB, Ghent (Belgium)
  3. Wisconsin Energy Institute, Madison, WI (United States)
  4. VIB, Ghent (Belgium)
  5. Univ. of Copenhagen, Frederiksberg C (Denmark); Carlsberg Research Lab., Copenhagen (Denmark)
Publication Date:
Grant/Contract Number:
FC02-07ER64494
Type:
Accepted Manuscript
Journal Name:
Plant Biotechnology Journal
Additional Journal Information:
Journal Volume: 15; Journal Issue: 5; Journal ID: ISSN 1467-7644
Publisher:
Society for Experimental Biology; Association of Applied Biology
Research Org:
Wisconsin Energy Institute, Madison, WI (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; biofuel; lignin modification; bacteria; Sphingomonas paucimobilis; Ligβ-aryl ether; saccharification yield
OSTI Identifier:
1393489

Mnich, Ewelina, Vanholme, Ruben, Oyarce, Paula, Liu, Sarah, Lu, Fachuang, Goeminne, Geert, Jorgensen, Bodil, Motawie, Mohammed S., Boerjan, Wout, Ralph, John, Ulvskov, Peter, Moller, Birger L., Bjarnholt, Nanna, and Harholt, Jesper. Degradation of lignin β-aryl ether units in Arabidopsis thaliana expressing LigD , LigF and LigG from Sphingomonas paucimobilis SYK-6. United States: N. p., Web. doi:10.1111/pbi.12655.
Mnich, Ewelina, Vanholme, Ruben, Oyarce, Paula, Liu, Sarah, Lu, Fachuang, Goeminne, Geert, Jorgensen, Bodil, Motawie, Mohammed S., Boerjan, Wout, Ralph, John, Ulvskov, Peter, Moller, Birger L., Bjarnholt, Nanna, & Harholt, Jesper. Degradation of lignin β-aryl ether units in Arabidopsis thaliana expressing LigD , LigF and LigG from Sphingomonas paucimobilis SYK-6. United States. doi:10.1111/pbi.12655.
Mnich, Ewelina, Vanholme, Ruben, Oyarce, Paula, Liu, Sarah, Lu, Fachuang, Goeminne, Geert, Jorgensen, Bodil, Motawie, Mohammed S., Boerjan, Wout, Ralph, John, Ulvskov, Peter, Moller, Birger L., Bjarnholt, Nanna, and Harholt, Jesper. 2016. "Degradation of lignin β-aryl ether units in Arabidopsis thaliana expressing LigD , LigF and LigG from Sphingomonas paucimobilis SYK-6". United States. doi:10.1111/pbi.12655. https://www.osti.gov/servlets/purl/1393489.
@article{osti_1393489,
title = {Degradation of lignin β-aryl ether units in Arabidopsis thaliana expressing LigD , LigF and LigG from Sphingomonas paucimobilis SYK-6},
author = {Mnich, Ewelina and Vanholme, Ruben and Oyarce, Paula and Liu, Sarah and Lu, Fachuang and Goeminne, Geert and Jorgensen, Bodil and Motawie, Mohammed S. and Boerjan, Wout and Ralph, John and Ulvskov, Peter and Moller, Birger L. and Bjarnholt, Nanna and Harholt, Jesper},
abstractNote = {Here, lignin is a major polymer in the secondary plant cell wall and composed of hydrophobic interlinked hydroxyphenylpropanoid units. The presence of lignin hampers conversion of plant biomass into biofuels; plants with modified lignin are therefore being investigated for increased digestibility. The bacterium Sphingomonas paucimobilis produces lignin-degrading enzymes including LigD, LigF and LigG involved in cleaving the most abundant lignin interunit linkage, the β-aryl ether bond. In this study, we expressed the LigD, LigF and LigG (LigDFG) genes in Arabidopsis thaliana to introduce postlignification modifications into the lignin structure. The three enzymes were targeted to the secretory pathway. Phenolic metabolite profiling and 2D HSQC NMR of the transgenic lines showed an increase in oxidized guaiacyl and syringyl units without concomitant increase in oxidized β-aryl ether units, showing lignin bond cleavage. Saccharification yield increased significantly in transgenic lines expressing LigDFG, showing the applicability of our approach. Additional new information on substrate specificity of the LigDFG enzymes is also provided.},
doi = {10.1111/pbi.12655},
journal = {Plant Biotechnology Journal},
number = 5,
volume = 15,
place = {United States},
year = {2016},
month = {10}
}

Works referenced in this record:

Lignin Biosynthesis
journal, June 2003

Dirigent phenoxy radical coupling advances and challenges
journal, August 2005

Enzymatic "Combustion": The Microbial Degradation of Lignin
journal, October 1987

The Genetics of Lignin Biosynthesis: Connecting Genotype to Phenotype
journal, December 2010

Chemoselective Metal-Free Aerobic Alcohol Oxidation in Lignin
journal, April 2013
  • Rahimi, Alireza; Azarpira, Ali; Kim, Hoon
  • Journal of the American Chemical Society, Vol. 135, Issue 17, p. 6415-6418
  • DOI: 10.1021/ja401793n

Lignins: Natural polymers from oxidative coupling of 4-hydroxyphenyl- propanoids
journal, January 2004

Lignin biosynthesis perturbations affect secondary cell wall composition and saccharification yield in Arabidopsis thaliana
journal, January 2013
  • Van Acker, Rebecca; Vanholme, Ruben; Storme, Véronique
  • Biotechnology for Biofuels, Vol. 6, Article No. 46
  • DOI: 10.1186/1754-6834-6-46

Disruption of Mediator rescues the stunted growth of a lignin-deficient Arabidopsis mutant
journal, March 2014
  • Bonawitz, Nicholas D.; Kim, Jeong Im; Tobimatsu, Yuki
  • Nature, Vol. 509, Issue 7500, p. 376-380
  • DOI: 10.1038/nature13084