Quantifying Carbon-14 for Biology Using Cavity Ring-Down Spectroscopy
Abstract
A cavity ring-down spectroscopy (CRDS) instrument was developed using mature, robust hardware for the measurement of carbon-14 in biological studies. The system was characterized using carbon-14 elevated glucose samples and returned a linear response up to 387 times contemporary carbon-14 concentrations. Carbon-14 free and contemporary carbon-14 samples with varying carbon-13 concentrations were used to assess the method detection limit of approximately one-third contemporary carbon-14 levels. Sources of inaccuracies are presented and discussed, and the capability to measure carbon-14 in biological samples is demonstrated by comparing pharmacokinetics from carbon-14 dosed guinea pigs analyzed by both CRDS and accelerator mass spectrometry. Here, the CRDS approach presented affords easy access to powerful carbon-14 tracer techniques that can characterize complex biochemical systems.
- Authors:
-
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Publication Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1367975
- Report Number(s):
- LLNL-JRNL-693042
Journal ID: ISSN 0003-2700
- Grant/Contract Number:
- AC52-07NA27344
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Analytical Chemistry
- Additional Journal Information:
- Journal Volume: 88; Journal Issue: 17; Journal ID: ISSN 0003-2700
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; 07 ISOTOPES AND RADIATION SOURCES
Citation Formats
McCartt, A. Daniel, Ognibene, Ted J., Bench, Graham, and Turteltaub, Kenneth W. Quantifying Carbon-14 for Biology Using Cavity Ring-Down Spectroscopy. United States: N. p., 2016.
Web. doi:10.1021/acs.analchem.6b02054.
McCartt, A. Daniel, Ognibene, Ted J., Bench, Graham, & Turteltaub, Kenneth W. Quantifying Carbon-14 for Biology Using Cavity Ring-Down Spectroscopy. United States. https://doi.org/10.1021/acs.analchem.6b02054
McCartt, A. Daniel, Ognibene, Ted J., Bench, Graham, and Turteltaub, Kenneth W. Tue .
"Quantifying Carbon-14 for Biology Using Cavity Ring-Down Spectroscopy". United States. https://doi.org/10.1021/acs.analchem.6b02054. https://www.osti.gov/servlets/purl/1367975.
@article{osti_1367975,
title = {Quantifying Carbon-14 for Biology Using Cavity Ring-Down Spectroscopy},
author = {McCartt, A. Daniel and Ognibene, Ted J. and Bench, Graham and Turteltaub, Kenneth W.},
abstractNote = {A cavity ring-down spectroscopy (CRDS) instrument was developed using mature, robust hardware for the measurement of carbon-14 in biological studies. The system was characterized using carbon-14 elevated glucose samples and returned a linear response up to 387 times contemporary carbon-14 concentrations. Carbon-14 free and contemporary carbon-14 samples with varying carbon-13 concentrations were used to assess the method detection limit of approximately one-third contemporary carbon-14 levels. Sources of inaccuracies are presented and discussed, and the capability to measure carbon-14 in biological samples is demonstrated by comparing pharmacokinetics from carbon-14 dosed guinea pigs analyzed by both CRDS and accelerator mass spectrometry. Here, the CRDS approach presented affords easy access to powerful carbon-14 tracer techniques that can characterize complex biochemical systems.},
doi = {10.1021/acs.analchem.6b02054},
journal = {Analytical Chemistry},
number = 17,
volume = 88,
place = {United States},
year = {Tue Jul 26 00:00:00 EDT 2016},
month = {Tue Jul 26 00:00:00 EDT 2016}
}
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