Re-directing bacterial microcompartment systems to enhance recombinant expression of lysis protein E from bacteriophage ϕX174 in Escherichia coli
Abstract
Recombinant expression of toxic proteins remains a challenging problem. Furthermore, one potential method to shield toxicity and thus improve expression of these proteins is to encapsulate them within protein compartments to sequester them away from their targets. Many bacteria naturally produce so-called bacterial microcompartments (BMCs) in which enzymes comprising a biosynthetic pathway are encapsulated in a proteinaeous shell, which is in part thought to shield the cells from the toxicity of reaction intermediates. As a proof-of-concept, we attempted to encapsulate toxic, lysis protein E (E) from bacteriophage ΦX174 inside recombinant BMCs to enhance its expression and achieve higher yields during downstream purification.
- Authors:
- Publication Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1618889
- Alternate Identifier(s):
- OSTI ID: 1357376
- Report Number(s):
- LLNL-JRNL-725506
Journal ID: ISSN 1475-2859; 71; PII: 685
- Grant/Contract Number:
- 15-LW-013; AC52-07NA27344
- Resource Type:
- Published Article
- Journal Name:
- Microbial Cell Factories
- Additional Journal Information:
- Journal Name: Microbial Cell Factories Journal Volume: 16 Journal Issue: 1; Journal ID: ISSN 1475-2859
- Publisher:
- Springer Science + Business Media
- Country of Publication:
- United Kingdom
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; bacterial microcompartment; BMC; lysis protein E; bacteriophage phiX174; toxic protein expression
Citation Formats
Yung, Mimi C., Bourguet, Feliza A., Carpenter, Timothy S., and Coleman, Matthew A. Re-directing bacterial microcompartment systems to enhance recombinant expression of lysis protein E from bacteriophage ϕX174 in Escherichia coli. United Kingdom: N. p., 2017.
Web. doi:10.1186/s12934-017-0685-x.
Yung, Mimi C., Bourguet, Feliza A., Carpenter, Timothy S., & Coleman, Matthew A. Re-directing bacterial microcompartment systems to enhance recombinant expression of lysis protein E from bacteriophage ϕX174 in Escherichia coli. United Kingdom. https://doi.org/10.1186/s12934-017-0685-x
Yung, Mimi C., Bourguet, Feliza A., Carpenter, Timothy S., and Coleman, Matthew A. Wed .
"Re-directing bacterial microcompartment systems to enhance recombinant expression of lysis protein E from bacteriophage ϕX174 in Escherichia coli". United Kingdom. https://doi.org/10.1186/s12934-017-0685-x.
@article{osti_1618889,
title = {Re-directing bacterial microcompartment systems to enhance recombinant expression of lysis protein E from bacteriophage ϕX174 in Escherichia coli},
author = {Yung, Mimi C. and Bourguet, Feliza A. and Carpenter, Timothy S. and Coleman, Matthew A.},
abstractNote = {Recombinant expression of toxic proteins remains a challenging problem. Furthermore, one potential method to shield toxicity and thus improve expression of these proteins is to encapsulate them within protein compartments to sequester them away from their targets. Many bacteria naturally produce so-called bacterial microcompartments (BMCs) in which enzymes comprising a biosynthetic pathway are encapsulated in a proteinaeous shell, which is in part thought to shield the cells from the toxicity of reaction intermediates. As a proof-of-concept, we attempted to encapsulate toxic, lysis protein E (E) from bacteriophage ΦX174 inside recombinant BMCs to enhance its expression and achieve higher yields during downstream purification.},
doi = {10.1186/s12934-017-0685-x},
journal = {Microbial Cell Factories},
number = 1,
volume = 16,
place = {United Kingdom},
year = {Wed Apr 26 00:00:00 EDT 2017},
month = {Wed Apr 26 00:00:00 EDT 2017}
}
https://doi.org/10.1186/s12934-017-0685-x
Web of Science
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