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Title: Soybean Roots Grown under Heat Stress Show Global Changes in Their Transcriptional and Proteomic Profiles

Heat stress is likely to be a key factor in the negative impact of climate change on crop production. Heat stress significantly influences the functions of roots, which provide support, water, and nutrients to other plant organs. Likewise, roots play an important role in the establishment of symbiotic associations with different microorganisms. Despite the physiological relevance of roots, few studies have examined their response to heat stress. Here in this study, we performed genome-wide transcriptomic and proteomic analyses on isolated root hairs, which are a single, epidermal cell type, and compared their response to stripped roots. On average, we identified 1849 and 3091 genes differentially regulated in root hairs and stripped roots, respectively, in response to heat stress. Our gene regulatory module analysis identified 10 key modules that might control the majority of the transcriptional response to heat stress. We also conducted proteomic analysis on membrane fractions isolated from root hairs and compared these responses to stripped roots. These experiments identified a variety of proteins whose expression changed within 3 h of application of heat stress. Most of these proteins were predicted to play a significant role in thermo-tolerance, as well as in chromatin remodeling and post-transcriptional regulation. In conclusion,more » the data presented represent an in-depth analysis of the heat stress response of a single cell type in soybean.« less
Authors:
 [1] ;  [2] ;  [2] ;  [2] ;  [3] ;  [4] ;  [5] ;  [6] ;  [7] ;  [7] ;  [7] ;  [7] ;  [2]
  1. Univ. of Missouri, Columbia, MO (United States). National Center for Soybean Biotechnology, C.S. Bond Life Sciences Center, Division of Plant Sciences and Biochemistry; Univ. Nacional Autonoma de Mexico (UNAM), Mexico City (Mexico). Lab. de Genomica Funcional de Leguminosas, Facultad de Estudios Superiores Iztacala
  2. Univ. of Missouri, Columbia, MO (United States). National Center for Soybean Biotechnology, C.S. Bond Life Sciences Center, Division of Plant Sciences and Biochemistry
  3. Univ. Nacional Autonoma de Mexico (UNAM), Mexico City (Mexico). Lab. de Genomica Funcional de Leguminosas, Facultad de Estudios Superiores Iztacalav
  4. Univ. of Missouri, Columbia, MO (United States). C.S. Bond Life Sciences Center, Informatics Inst.
  5. Univ. of Missouri, Columbia, MO (United States). C.S. Bond Life Sciences Center, Informatics Inst.; Univ. of Missouri, Columbia, MO (United States). Dept. of Computer Science; Univ. of Missouri, Columbia, MO (United States). School of Medicine, Dept. of Molecular Microbiology and Immunology and Office of Research
  6. Univ. of Missouri, Columbia, MO (United States). C.S. Bond Life Sciences Center, Informatics Inst.; Univ. of Missouri, Columbia, MO (United States). Dept. of Computer Science
  7. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
Publication Date:
Report Number(s):
PNNL-SA-114787
Journal ID: ISSN 1664-462X
Grant/Contract Number:
SC0004898; AC05-76RL01830; 219759; 252260; IA203815; FESI-DIP-PAPCA-2014-3
Type:
Accepted Manuscript
Journal Name:
Frontiers in Plant Science
Additional Journal Information:
Journal Volume: 7; Journal ID: ISSN 1664-462X
Publisher:
Frontiers Research Foundation
Research Org:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org:
USDOE Office of Science (SC); USDOE Laboratory Directed Research and Development (LDRD) Program
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; soybean; roothairs; heatstress; genemodule; transcriptomics; proteomics
OSTI Identifier:
1357046