Ebola Virus VP35 Interaction with Dynein LC8 Regulates Viral RNA Synthesis

Luthra, Priya; Jordan, David S.; Leung, Daisy W.; Amarasinghe, Gaya K.; Basler, Christopher F.

doi:10.1128/JVI.03652-14

Title: Ebola Virus VP35 Interaction with Dynein LC8 Regulates Viral RNA Synthesis

Abstract

Ebola virus VP35 inhibits alpha/beta interferon production and functions as a viral polymerase cofactor. Previously, the 8-kDa cytoplasmic dynein light chain (LC8) was demonstrated to interact with VP35, but the functional consequences were unclear. Furthermore we demonstrate that the interaction is direct and of high affinity and that binding stabilizes the VP35 N-terminal oligomerization domain and enhances viral RNA synthesis. Mutational analysis demonstrates that VP35 interaction is required for the functional effects of LC8.

Authors:

Luthra, Priya ^[1]; Jordan, David S. ^[2]; Leung, Daisy W. ^[2]; Amarasinghe, Gaya K. ^[2]; Basler, Christopher F. ^[1]

Icahn School of Medicine at Mount Sinai, New York, NY (United States)
Washington Univ. School of Medicine, St. Louis, MO (United States)

Publication Date:: Fri Apr 03 00:00:00 EDT 2015

Research Org.:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Org.:: National Institutes of Health (NIH)

OSTI Identifier:: 1351394

Grant/Contract Number:: R01AI107056; R01AI059536; U19AI109945; U19AI109664; U19 AI070489; R01AI081914; T32-CA09547-37

Resource Type:: Accepted Manuscript

Journal Name:: Journal of Virology

Additional Journal Information:: Journal Volume: 89; Journal Issue: 9; Journal ID: ISSN 0022-538X

Publisher:: American Society for Microbiology

Country of Publication:: United States

Language:: ENGLISH

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES

Citation Formats


                    Luthra, Priya, Jordan, David S., Leung, Daisy W., Amarasinghe, Gaya K., and Basler, Christopher F. Ebola Virus VP35 Interaction with Dynein LC8 Regulates Viral RNA Synthesis.  United States: N. p., 2015. 
Web.  doi:10.1128/JVI.03652-14.

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                    Luthra, Priya, Jordan, David S., Leung, Daisy W., Amarasinghe, Gaya K., & Basler, Christopher F. Ebola Virus VP35 Interaction with Dynein LC8 Regulates Viral RNA Synthesis.  United States.  https://doi.org/10.1128/JVI.03652-14

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                    Luthra, Priya, Jordan, David S., Leung, Daisy W., Amarasinghe, Gaya K., and Basler, Christopher F. Fri .  
"Ebola Virus VP35 Interaction with Dynein LC8 Regulates Viral RNA Synthesis".  United States.  https://doi.org/10.1128/JVI.03652-14.  https://www.osti.gov/servlets/purl/1351394.

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@article{osti_1351394,

  title        = {Ebola Virus VP35 Interaction with Dynein LC8 Regulates Viral RNA Synthesis},

  author       = {Luthra, Priya and Jordan, David S. and Leung, Daisy W. and Amarasinghe, Gaya K. and Basler, Christopher F.},

  abstractNote = {Ebola virus VP35 inhibits alpha/beta interferon production and functions as a viral polymerase cofactor. Previously, the 8-kDa cytoplasmic dynein light chain (LC8) was demonstrated to interact with VP35, but the functional consequences were unclear. Furthermore we demonstrate that the interaction is direct and of high affinity and that binding stabilizes the VP35 N-terminal oligomerization domain and enhances viral RNA synthesis. Mutational analysis demonstrates that VP35 interaction is required for the functional effects of LC8.},

  doi          = {10.1128/JVI.03652-14},

  journal      = {Journal of Virology},

  number       = 9,

  volume       = 89,

  place        = {United States},

  year         = {Fri Apr 03 00:00:00 EDT 2015},

  month        = {Fri Apr 03 00:00:00 EDT 2015}

}

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Title: Ebola Virus VP35 Interaction with Dynein LC8 Regulates Viral RNA Synthesis

Abstract

Citation Formats

Structural models of DYNLL1 with interacting partners: African swine fever virus protein p54 and postsynaptic scaffolding protein gephyrin journal, November 2010

Recognition of novel viral sequences that associate with the dynein light chain LC8 identified through a pepscan technique journal, May 2003

Dimerization of the Highly Conserved Light Chain Shared by Dynein and Myosin V journal, August 1997

Cytoplasmic Dynein Contains a Family of Differentially Expressed Light Chains † journal, October 1998

Viral immune modulators perturb the human molecular network by common and unique strategies journal, July 2012

African Swine Fever Virus Protein p54 Interacts with the Microtubular Motor Complex through Direct Binding to Light-Chain Dynein journal, October 2001

The dynein light chain 8 binding motif of rabies virus phosphoprotein promotes efficient viral transcription journal, April 2007

Both matrix proteins of Ebola virus contribute to the regulation of viral genome replication and transcription journal, July 2010

Structure of the Ebola VP35 interferon inhibitory domain journal, January 2009

Mutual Antagonism between the Ebola Virus VP35 Protein and the RIG-I Activator PACT Determines Infection Outcome journal, July 2013

The association of viral proteins with host cell dynein components during virus infection journal, August 2011

The Ebola virus ribonucleoprotein complex: A novel VP30–L interaction identified journal, March 2009

The Ebola virus VP35 protein functions as a type I IFN antagonist journal, October 2000

A C-terminal basic amino acid motif of Zaire ebolavirus VP35 is essential for type I interferon antagonism and displays high identity with the RNA-binding domain of another interferon antagonist, the NS1 protein of influenza A virus journal, October 2004

Nucleocapsid formation and RNA synthesis of Marburg virus is dependent on two coiled coil motifs in the nucleoprotein journal, January 2007

Herpes Simplex Virus Type 1 Capsid Protein VP26 Interacts with Dynein Light Chains RP3 and Tctex1 and Plays a Role in Retrograde Cellular Transport journal, April 2004

The 8-kDa Dynein Light Chain Binds to Its Targets via a Conserved (K/R) X TQT Motif journal, January 2001

Interactions of Marburg Virus Nucleocapsid Proteins journal, September 1998

Structure of the Monomeric 8-kDa Dynein Light Chain and Mechanism of the Domain-swapped Dimer Assembly journal, August 2003

The Ebola Virus VP35 Protein Inhibits Activation of Interferon Regulatory Factor 3 journal, July 2003

Ebolavirus VP35 Interacts with the Cytoplasmic Dynein Light Chain 8 journal, April 2009

Identification of novel cellular proteins that bind to the LC8 dynein light chain using a pepscan technique journal, August 2001

Identification of the dynein light chains required for human papillomavirus infection: Role of dynein light chains in HPV infection journal, December 2010

Ebolavirus VP35 is a multifunctional virulence factor journal, November 2010

Homo-oligomerization facilitates the interferon-antagonist activity of the ebolavirus VP35 protein journal, October 2005

Interaction of the Rabies Virus P Protein with the LC8 Dynein Light Chain journal, November 2000

On the Domain Structure and the Polymerization State of the Sendai Virus P Protein journal, January 2000

Comparison of the Transcription and Replication Strategies of Marburg Virus and Ebola Virus by Using Artificial Replication Systems journal, March 1999

How order and disorder within paramyxoviral nucleoproteins and phosphoproteins orchestrate the molecular interplay of transcription and replication journal, June 2017

Human transbodies that interfere with the functions of Ebola virus VP35 protein in genome replication and transcription and innate immune antagonism journal, December 2018

Ebola virus VP35 has novel NTPase and helicase-like activities journal, May 2019

RNA Binding of Ebola Virus VP30 Is Essential for Activating Viral Transcription journal, June 2016

Measles Virus Forms Inclusion Bodies with Properties of Liquid Organelles journal, August 2019

Ebolaviruses: New roles for old proteins journal, May 2018

Identification of RUVBL1 and RUVBL2 as Novel Cellular Interactors of the Ebola Virus Nucleoprotein journal, April 2019

Investigating the Cellular Transcriptomic Response Induced by the Makona Variant of Ebola Virus in Differentiated THP-1 Cells journal, November 2019

Structural models of DYNLL1 with interacting partners: African swine fever virus protein p54 and postsynaptic scaffolding protein gephyrin
journal, November 2010

Recognition of novel viral sequences that associate with the dynein light chain LC8 identified through a pepscan technique
journal, May 2003

Dimerization of the Highly Conserved Light Chain Shared by Dynein and Myosin V
journal, August 1997

Cytoplasmic Dynein Contains a Family of Differentially Expressed Light Chains ^†
journal, October 1998

Viral immune modulators perturb the human molecular network by common and unique strategies
journal, July 2012

African Swine Fever Virus Protein p54 Interacts with the Microtubular Motor Complex through Direct Binding to Light-Chain Dynein
journal, October 2001

The dynein light chain 8 binding motif of rabies virus phosphoprotein promotes efficient viral transcription
journal, April 2007

Both matrix proteins of Ebola virus contribute to the regulation of viral genome replication and transcription
journal, July 2010

Structure of the Ebola VP35 interferon inhibitory domain
journal, January 2009

Mutual Antagonism between the Ebola Virus VP35 Protein and the RIG-I Activator PACT Determines Infection Outcome
journal, July 2013

The association of viral proteins with host cell dynein components during virus infection
journal, August 2011

The Ebola virus ribonucleoprotein complex: A novel VP30–L interaction identified
journal, March 2009

The Ebola virus VP35 protein functions as a type I IFN antagonist
journal, October 2000

A C-terminal basic amino acid motif of Zaire ebolavirus VP35 is essential for type I interferon antagonism and displays high identity with the RNA-binding domain of another interferon antagonist, the NS1 protein of influenza A virus
journal, October 2004

Nucleocapsid formation and RNA synthesis of Marburg virus is dependent on two coiled coil motifs in the nucleoprotein
journal, January 2007

Herpes Simplex Virus Type 1 Capsid Protein VP26 Interacts with Dynein Light Chains RP3 and Tctex1 and Plays a Role in Retrograde Cellular Transport
journal, April 2004

The 8-kDa Dynein Light Chain Binds to Its Targets via a Conserved (K/R) X TQT Motif
journal, January 2001

Interactions of Marburg Virus Nucleocapsid Proteins
journal, September 1998

Structure of the Monomeric 8-kDa Dynein Light Chain and Mechanism of the Domain-swapped Dimer Assembly
journal, August 2003

The Ebola Virus VP35 Protein Inhibits Activation of Interferon Regulatory Factor 3
journal, July 2003

Ebolavirus VP35 Interacts with the Cytoplasmic Dynein Light Chain 8
journal, April 2009

Identification of novel cellular proteins that bind to the LC8 dynein light chain using a pepscan technique
journal, August 2001

Identification of the dynein light chains required for human papillomavirus infection: Role of dynein light chains in HPV infection
journal, December 2010

Ebolavirus VP35 is a multifunctional virulence factor
journal, November 2010

Homo-oligomerization facilitates the interferon-antagonist activity of the ebolavirus VP35 protein
journal, October 2005

Interaction of the Rabies Virus P Protein with the LC8 Dynein Light Chain
journal, November 2000

On the Domain Structure and the Polymerization State of the Sendai Virus P Protein
journal, January 2000

Comparison of the Transcription and Replication Strategies of Marburg Virus and Ebola Virus by Using Artificial Replication Systems
journal, March 1999

How order and disorder within paramyxoviral nucleoproteins and phosphoproteins orchestrate the molecular interplay of transcription and replication
journal, June 2017

Human transbodies that interfere with the functions of Ebola virus VP35 protein in genome replication and transcription and innate immune antagonism
journal, December 2018

Ebola virus VP35 has novel NTPase and helicase-like activities
journal, May 2019

RNA Binding of Ebola Virus VP30 Is Essential for Activating Viral Transcription
journal, June 2016

Measles Virus Forms Inclusion Bodies with Properties of Liquid Organelles
journal, August 2019

Ebolaviruses: New roles for old proteins
journal, May 2018

Identification of RUVBL1 and RUVBL2 as Novel Cellular Interactors of the Ebola Virus Nucleoprotein
journal, April 2019

Investigating the Cellular Transcriptomic Response Induced by the Makona Variant of Ebola Virus in Differentiated THP-1 Cells
journal, November 2019