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Title: Characterizing the lipid and metabolite changes associated with placental function and pregnancy complications using ion mobility spectrometry-mass spectrometry and mass spectrometry imaging

A successful pregnancy is dependent upon discrete biological events, which include embryo implantation, decidualization, and placentation. Furthermore, problems associated with each of these events can cause infertility or conditions such as preeclampsia. A greater understanding of the molecular changes associated with these complex processes is necessary to aid in identifying treatments for each condition. Previous nuclear magnetic resonance spectroscopy and mass spectrometry studies have been used to identify metabolites and lipids associated with pregnancy-related complications. However, due to limitations associated with conventional implementations of both techniques, novel technology developments are needed to more fully understand the initiation and development of pregnancy related problems at the molecular level. Here, we describe current analytical techniques for metabolomic and lipidomic characterization of pregnancy complications and discuss the potential for new technologies such as ion mobility spectrometry-mass spectrometry and mass spectrometry imaging to contribute to a better understanding of the molecular changes that affect the placenta and pregnancy outcomes.
Authors:
 [1] ;  [1] ; ORCiD logo [1]
  1. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Biological Sciences Division
Publication Date:
Report Number(s):
PNNL-SA-123563
Journal ID: ISSN 0143-4004; PII: S0143400417302175
Grant/Contract Number:
AC0576Rl01830; R01 ES022190; R21 HD084788; AC05-76RL01830
Type:
Accepted Manuscript
Journal Name:
Placenta (Eastbourne)
Additional Journal Information:
Journal Name: Placenta (Eastbourne); Journal Volume: 60; Journal Issue: S1; Journal ID: ISSN 0143-4004
Research Org:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; deep placentation; pregnancy complications; mass spectrometry imaging; metabolome; lipidome; ion mobility spectrometry- mass spectrometry; Nano-DESI
OSTI Identifier:
1349079