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Title: Surface Characterization of Populus during Caldicellulosiruptor bescii Growth by TOF-SIMS Analysis

Abstract

Caldicellulosiruptor bescii is a thermophilic, anaerobic bacterium that is capable of utilizing unpretreated biomass in addition to breaking down cellulose and hemicellulose into simple sugars. Despite the fact that C. bescii must first bind to the surface of the biomass, there has been no analysis of the morphological or chemical changes to the biomass surface as a result of incubation with the micro-organism. To understand more about C. bescii growth, juvenile poplar stems were sectioned (80 μm thick) and incubated with C. bescii beyond the typical 24 h experiment length. Monitoring the cell counts during incubation revealed a biphasic growth pattern. The impact the micro-organism had on the surface was determined by scanning electron microscopy (SEM) and time-of-flight secondary ion mass spectrometry (ToF-SIMS), which showed physical crevices in the cell wall caused by the C. bescii along with a decrease of polysaccharide ions and an increase in lignin ions on the poplar surface. Employing infrared microspectroscopy, the decreasing trend was corroborated.

Authors:
 [1];  [2];  [1];  [2];  [3];  [2];  [4]
+ Show Author Affiliations
  1. Georgia Inst. of Technology, Atlanta, GA (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. Univ. of Georgia, Athens, GA (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1348339
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
ACS Sustainable Chemistry & Engineering
Additional Journal Information:
Journal Volume: 5; Journal Issue: 3; Journal ID: ISSN 2168-0485
Publisher:
American Chemical Society (ACS)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Tolbert, Allison K., Young, Jenna M., Jung, Seokwon, Chung, Daehwan, Passian, Ali, Westpheling, Janet, and Ragauskus, Arthur J. Surface Characterization of Populus during Caldicellulosiruptor bescii Growth by TOF-SIMS Analysis. United States: N. p., 2017. Web. doi:10.1021/acssuschemeng.6b01877.
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Tolbert, Allison K., Young, Jenna M., Jung, Seokwon, Chung, Daehwan, Passian, Ali, Westpheling, Janet, & Ragauskus, Arthur J. Surface Characterization of Populus during Caldicellulosiruptor bescii Growth by TOF-SIMS Analysis. United States. https://doi.org/10.1021/acssuschemeng.6b01877
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Tolbert, Allison K., Young, Jenna M., Jung, Seokwon, Chung, Daehwan, Passian, Ali, Westpheling, Janet, and Ragauskus, Arthur J. Mon . "Surface Characterization of Populus during Caldicellulosiruptor bescii Growth by TOF-SIMS Analysis". United States. https://doi.org/10.1021/acssuschemeng.6b01877. https://www.osti.gov/servlets/purl/1348339.
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@article{osti_1348339,
title = {Surface Characterization of Populus during Caldicellulosiruptor bescii Growth by TOF-SIMS Analysis},
author = {Tolbert, Allison K. and Young, Jenna M. and Jung, Seokwon and Chung, Daehwan and Passian, Ali and Westpheling, Janet and Ragauskus, Arthur J.},
abstractNote = {Caldicellulosiruptor bescii is a thermophilic, anaerobic bacterium that is capable of utilizing unpretreated biomass in addition to breaking down cellulose and hemicellulose into simple sugars. Despite the fact that C. bescii must first bind to the surface of the biomass, there has been no analysis of the morphological or chemical changes to the biomass surface as a result of incubation with the micro-organism. To understand more about C. bescii growth, juvenile poplar stems were sectioned (80 μm thick) and incubated with C. bescii beyond the typical 24 h experiment length. Monitoring the cell counts during incubation revealed a biphasic growth pattern. The impact the micro-organism had on the surface was determined by scanning electron microscopy (SEM) and time-of-flight secondary ion mass spectrometry (ToF-SIMS), which showed physical crevices in the cell wall caused by the C. bescii along with a decrease of polysaccharide ions and an increase in lignin ions on the poplar surface. Employing infrared microspectroscopy, the decreasing trend was corroborated.},
doi = {10.1021/acssuschemeng.6b01877},
journal = {ACS Sustainable Chemistry & Engineering},
number = 3,
volume = 5,
place = {United States},
year = {Mon Jan 30 00:00:00 EST 2017},
month = {Mon Jan 30 00:00:00 EST 2017}
}
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https://doi.org/10.1021/acssuschemeng.6b01877
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    Works referencing / citing this record:

    Cleaning carbohydrate impurities from lignin using Pseudomonas fluorescens
    journal, January 2019

    • Ghosh, Tanushree; Ngo, Tri-Dung; Kumar, Aloke
    • Green Chemistry, Vol. 21, Issue 7
    • DOI: 10.1039/c8gc03341b
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