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Title: Dynamic crystallography reveals early signalling events in ultraviolet photoreceptor UVR8

Abstract

Arabidopsis thaliana UVR8 (AtUVR8) is a long-sought-after photoreceptor that undergoes dimer dissociation in response to UV-B light. Crystallographic and mutational studies have identified two crucial tryptophan residues for UV-B responses in AtUVR8. However, the mechanism of UV-B perception and structural events leading up to dimer dissociation remain elusive at the molecular level. We applied dynamic crystallography to capture light-induced structural events in photoactive AtUVR8 crystals. Here we report two intermediate structures at 1.67Å resolution. At the epicenter of UV-B signaling, concerted motions associated with Trp285/Trp233 lead to ejection of a water molecule, which weakens an intricate network of hydrogen bonds and salt bridges at the dimer interface. Partial opening of the β-propeller structure due to thermal relaxation of conformational strains originating in the epicenter further disrupts the dimer interface and leads to dimer dissociation. Ultimately, these dynamic crystallographic observations provide structural insights into the photo-perception and signaling mechanism of UVR8.

Authors:
 [1];  [2];  [3];  [3];  [1];  [1];  [3];  [1];  [4]
  1. Huazhong Agricultural Univ., Hubei (People's Republic of China)
  2. Renz Research Inc., Westmont, IL (United States)
  3. City Univ. of Hong Kong, Kowloon (Hong Kong)
  4. The Univ. of Chicago, Chicago, IL (United States)
Publication Date:
Research Org.:
UChicago, LLC., Argonne, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1347118
Grant/Contract Number:  
AC02-06CH11357; GM036452; EY024363; 31270893; 31110103912
Resource Type:
Accepted Manuscript
Journal Name:
Nature Plants
Additional Journal Information:
Journal Volume: 1; Journal Issue: 1; Journal ID: ISSN 2055-0278
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 54 ENVIRONMENTAL SCIENCES; photoreceptor; light signaling; dynamic crystallography; UVR8; photo-perception mechanism

Citation Formats

Zeng, Xiaoli, Ren, Zhong, Wu, Qi, Fan, Jun, Peng, Pan -Pan, Tang, Kun, Zhang, Ruiqin, Zhao, Kai -Hong, and Yang, Xiaojing. Dynamic crystallography reveals early signalling events in ultraviolet photoreceptor UVR8. United States: N. p., 2015. Web. doi:10.1038/nplants.2014.6.
Zeng, Xiaoli, Ren, Zhong, Wu, Qi, Fan, Jun, Peng, Pan -Pan, Tang, Kun, Zhang, Ruiqin, Zhao, Kai -Hong, & Yang, Xiaojing. Dynamic crystallography reveals early signalling events in ultraviolet photoreceptor UVR8. United States. doi:10.1038/nplants.2014.6.
Zeng, Xiaoli, Ren, Zhong, Wu, Qi, Fan, Jun, Peng, Pan -Pan, Tang, Kun, Zhang, Ruiqin, Zhao, Kai -Hong, and Yang, Xiaojing. Thu . "Dynamic crystallography reveals early signalling events in ultraviolet photoreceptor UVR8". United States. doi:10.1038/nplants.2014.6. https://www.osti.gov/servlets/purl/1347118.
@article{osti_1347118,
title = {Dynamic crystallography reveals early signalling events in ultraviolet photoreceptor UVR8},
author = {Zeng, Xiaoli and Ren, Zhong and Wu, Qi and Fan, Jun and Peng, Pan -Pan and Tang, Kun and Zhang, Ruiqin and Zhao, Kai -Hong and Yang, Xiaojing},
abstractNote = {Arabidopsis thaliana UVR8 (AtUVR8) is a long-sought-after photoreceptor that undergoes dimer dissociation in response to UV-B light. Crystallographic and mutational studies have identified two crucial tryptophan residues for UV-B responses in AtUVR8. However, the mechanism of UV-B perception and structural events leading up to dimer dissociation remain elusive at the molecular level. We applied dynamic crystallography to capture light-induced structural events in photoactive AtUVR8 crystals. Here we report two intermediate structures at 1.67Å resolution. At the epicenter of UV-B signaling, concerted motions associated with Trp285/Trp233 lead to ejection of a water molecule, which weakens an intricate network of hydrogen bonds and salt bridges at the dimer interface. Partial opening of the β-propeller structure due to thermal relaxation of conformational strains originating in the epicenter further disrupts the dimer interface and leads to dimer dissociation. Ultimately, these dynamic crystallographic observations provide structural insights into the photo-perception and signaling mechanism of UVR8.},
doi = {10.1038/nplants.2014.6},
journal = {Nature Plants},
number = 1,
volume = 1,
place = {United States},
year = {2015},
month = {1}
}

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Cited by: 15 works
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