UV-B photoreceptor-mediated protection of the photosynthetic machinery in Chlamydomonas reinhardtii
Abstract
Life on earth is dependent on the photosynthetic conversion of light energy into chemical energy. However, absorption of excess sunlight can damage the photosynthetic machinery and limit photosynthetic activity, thereby affecting growth and productivity. Photosynthetic light harvesting can be down-regulated by nonphotochemical quenching (NPQ). A major component of NPQ is qE (energy-dependent nonphotochemical quenching), which allows dissipation of light energy as heat. Photodamage peaks in the UV-B part of the spectrum, but whether and how UV-B induces qE are unknown. Plants are responsive to UV-B via the UVR8 photoreceptor. Here in this paper, we report in the green alga Chlamydomonas reinhardtii that UVR8 induces accumulation of specific members of the light-harvesting complex (LHC) superfamily that contribute to qE, in particular LHC Stress-Related 1 (LHCSR1) and Photosystem II Subunit S (PSBS). The capacity for qE is strongly induced by UV-B, although the patterns of qE-related proteins accumulating in response to UV-B or to high light are clearly different. The competence for qE induced by acclimation to UV-B markedly contributes to photoprotection upon subsequent exposure to high light. Our study reveals an anterograde link between photoreceptor-mediated signaling in the nucleocytosolic compartment and the photoprotective regulation of photosynthetic activity in the chloroplast.
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); European Union (EU); Gordon and Betty Moore Foundation; Swiss National Science Foundation (SNSF)
- OSTI Identifier:
- 1334548
- Alternate Identifier(s):
- OSTI ID: 1411563
- Grant/Contract Number:
- AC02-05CH11231; GBMF3070; 31003A_153475; 31003A_146300
- Resource Type:
- Published Article
- Journal Name:
- Proceedings of the National Academy of Sciences of the United States of America
- Additional Journal Information:
- Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Volume: 113 Journal Issue: 51; Journal ID: ISSN 0027-8424
- Publisher:
- National Academy of Sciences, Washington, DC (United States)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 60 APPLIED LIFE SCIENCES; photoprotection; nonphotochemical quenching; UV-B photoreceptor; PSBS; LHCSR1
Citation Formats
Allorent, Guillaume, Lefebvre-Legendre, Linnka, Chappuis, Richard, Kuntz, Marcel, Truong, Thuy B., Niyogi, Krishna K., Ulm, Roman, and Goldschmidt-Clermont, Michel. UV-B photoreceptor-mediated protection of the photosynthetic machinery in Chlamydomonas reinhardtii. United States: N. p., 2016.
Web. doi:10.1073/pnas.1607695114.
Allorent, Guillaume, Lefebvre-Legendre, Linnka, Chappuis, Richard, Kuntz, Marcel, Truong, Thuy B., Niyogi, Krishna K., Ulm, Roman, & Goldschmidt-Clermont, Michel. UV-B photoreceptor-mediated protection of the photosynthetic machinery in Chlamydomonas reinhardtii. United States. https://doi.org/10.1073/pnas.1607695114
Allorent, Guillaume, Lefebvre-Legendre, Linnka, Chappuis, Richard, Kuntz, Marcel, Truong, Thuy B., Niyogi, Krishna K., Ulm, Roman, and Goldschmidt-Clermont, Michel. Mon .
"UV-B photoreceptor-mediated protection of the photosynthetic machinery in Chlamydomonas reinhardtii". United States. https://doi.org/10.1073/pnas.1607695114.
@article{osti_1334548,
title = {UV-B photoreceptor-mediated protection of the photosynthetic machinery in Chlamydomonas reinhardtii},
author = {Allorent, Guillaume and Lefebvre-Legendre, Linnka and Chappuis, Richard and Kuntz, Marcel and Truong, Thuy B. and Niyogi, Krishna K. and Ulm, Roman and Goldschmidt-Clermont, Michel},
abstractNote = {Life on earth is dependent on the photosynthetic conversion of light energy into chemical energy. However, absorption of excess sunlight can damage the photosynthetic machinery and limit photosynthetic activity, thereby affecting growth and productivity. Photosynthetic light harvesting can be down-regulated by nonphotochemical quenching (NPQ). A major component of NPQ is qE (energy-dependent nonphotochemical quenching), which allows dissipation of light energy as heat. Photodamage peaks in the UV-B part of the spectrum, but whether and how UV-B induces qE are unknown. Plants are responsive to UV-B via the UVR8 photoreceptor. Here in this paper, we report in the green alga Chlamydomonas reinhardtii that UVR8 induces accumulation of specific members of the light-harvesting complex (LHC) superfamily that contribute to qE, in particular LHC Stress-Related 1 (LHCSR1) and Photosystem II Subunit S (PSBS). The capacity for qE is strongly induced by UV-B, although the patterns of qE-related proteins accumulating in response to UV-B or to high light are clearly different. The competence for qE induced by acclimation to UV-B markedly contributes to photoprotection upon subsequent exposure to high light. Our study reveals an anterograde link between photoreceptor-mediated signaling in the nucleocytosolic compartment and the photoprotective regulation of photosynthetic activity in the chloroplast.},
doi = {10.1073/pnas.1607695114},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = 51,
volume = 113,
place = {United States},
year = {Mon Dec 05 00:00:00 EST 2016},
month = {Mon Dec 05 00:00:00 EST 2016}
}
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https://doi.org/10.1073/pnas.1607695114
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