Fermentation of glycerol by Anaerobium acetethylicum and its potential use in biofuel production

Patil, Yogita; Junghare, Madan; Müller, Nicolai

doi:10.1111/1751-7915.12484

Title: Fermentation of glycerol by Anaerobium acetethylicum and its potential use in biofuel production

Abstract

Summary Growth of biodiesel industries resulted in increased coproduction of crude glycerol which is therefore becoming a waste product instead of a valuable ‘coproduct’. Glycerol can be used for the production of valuable chemicals, e.g. biofuels, to reduce glycerol waste disposal. In this study, a novel bacterial strain is described which converts glycerol mainly to ethanol and hydrogen with very little amounts of acetate, formate and 1,2‐propanediol as coproducts. The bacterium offers certain advantages over previously studied glycerol‐fermenting microorganisms. Anaerobium acetethylicum during growth with glycerol produces very little side products and grows in the presence of maximum glycerol concentrations up to 1500 mM and in the complete absence of complex organic supplements such as yeast extract or tryptone. The highest observed growth rate of 0.116 h ⁻¹ is similar to that of other glycerol degraders, and the maximum concentration of ethanol that can be tolerated was found to be about 60 mM (2.8 g l ⁻¹ ) and further growth was likely inhibited due to ethanol toxicity. Proteome analysis as well as enzyme assays performed in cell‐free extracts demonstrated that glycerol is degraded via glyceraldehyde‐3‐phosphate, which is further metabolized through the lower part of glycolysis leading to formation of mainly ethanol and hydrogen. In conclusion,more »« less

Authors:

Patil, Yogita ^[1]; Junghare, Madan ^[2]; Müller, Nicolai ^[1]

Department of Biology, Microbial Ecology University of Konstanz Konstanz D‐78457 Germany
Department of Biology, Microbial Ecology University of Konstanz Konstanz D‐78457 Germany, Konstanz Research School of Chemical Biology University of Konstanz Konstanz D‐78457 Germany

Publication Date:: Thu Dec 22 00:00:00 EST 2016

Research Org.:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Univ. of Konstanz (Germany)

Sponsoring Org.:: USDOE Office of Science (SC)

OSTI Identifier:: 1337343

Alternate Identifier(s):: OSTI ID: 1341325; OSTI ID: 1625854

Grant/Contract Number:: AC02-05CH11231

Resource Type:: Published Article

Journal Name:: Microbial Biotechnology (Online)

Additional Journal Information:: Journal Name: Microbial Biotechnology (Online) Journal Volume: 10 Journal Issue: 1; Journal ID: ISSN 1751-7915

Publisher:: Wiley-Blackwell

Country of Publication:: United Kingdom

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; Biotechnology & Applied Microbiology; Microbiology

Citation Formats


                    Patil, Yogita, Junghare, Madan, and Müller, Nicolai. Fermentation of glycerol by Anaerobium acetethylicum and its potential use in biofuel production.  United Kingdom: N. p., 2016. 
Web.  doi:10.1111/1751-7915.12484.

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                    Patil, Yogita, Junghare, Madan, & Müller, Nicolai. Fermentation of glycerol by Anaerobium acetethylicum and its potential use in biofuel production.  United Kingdom.  https://doi.org/10.1111/1751-7915.12484

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                    Patil, Yogita, Junghare, Madan, and Müller, Nicolai. Thu .  
"Fermentation of glycerol by Anaerobium acetethylicum and its potential use in biofuel production".  United Kingdom.  https://doi.org/10.1111/1751-7915.12484.

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@article{osti_1337343,

  title        = {Fermentation of glycerol by Anaerobium acetethylicum and its potential use in biofuel production},

  author       = {Patil, Yogita and Junghare, Madan and Müller, Nicolai},

  abstractNote = {Summary Growth of biodiesel industries resulted in increased coproduction of crude glycerol which is therefore becoming a waste product instead of a valuable ‘coproduct’. Glycerol can be used for the production of valuable chemicals, e.g. biofuels, to reduce glycerol waste disposal. In this study, a novel bacterial strain is described which converts glycerol mainly to ethanol and hydrogen with very little amounts of acetate, formate and 1,2‐propanediol as coproducts. The bacterium offers certain advantages over previously studied glycerol‐fermenting microorganisms. Anaerobium acetethylicum during growth with glycerol produces very little side products and grows in the presence of maximum glycerol concentrations up to 1500 mM and in the complete absence of complex organic supplements such as yeast extract or tryptone. The highest observed growth rate of 0.116 h −1 is similar to that of other glycerol degraders, and the maximum concentration of ethanol that can be tolerated was found to be about 60 mM (2.8 g l −1 ) and further growth was likely inhibited due to ethanol toxicity. Proteome analysis as well as enzyme assays performed in cell‐free extracts demonstrated that glycerol is degraded via glyceraldehyde‐3‐phosphate, which is further metabolized through the lower part of glycolysis leading to formation of mainly ethanol and hydrogen. In conclusion, fermentation of glycerol to ethanol and hydrogen by this bacterium represents a remarkable option to add value to the biodiesel industries by utilization of surplus glycerol.},

  doi          = {10.1111/1751-7915.12484},

  journal      = {Microbial Biotechnology (Online)},

  number       = 1,

  volume       = 10,

  place        = {United Kingdom},

  year         = {Thu Dec 22 00:00:00 EST 2016},

  month        = {Thu Dec 22 00:00:00 EST 2016}

}

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Figures / Tables:

Fig. 1: Growth of Anaerobium acetethylicum at different initial concentrations of glycerol incubated at 30°C for 118 h. Shown are mean values of triplicates ± standard deviations, except for 1500 mM of glycerol (n = 2). A. black circles: 10 mM of glycerol, black diamonds: 50 mM of glycerol, blackmore »

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Title: Fermentation of glycerol by Anaerobium acetethylicum and its potential use in biofuel production

Abstract

Citation Formats

Figures / Tables:

Optimization of cultural conditions for conversion of glycerol to ethanol by Enterobacter aerogenes S012 journal, January 2013

Ethanol Tolerance in the Yeast Saccharomyces cerevisiae Is Dependent on Cellular Oleic Acid Content journal, March 2003

Bioconversion of crude glycerol by fungi journal, February 2012

The Genomes On Line Database (GOLD) in 2007: status of genomic and metagenomic projects and their associated metadata journal, December 2007

Glycerol facilitator of Escherichia coli: cloning of glpF and identification of the glpF product. journal, January 1990

Glycerol: A promising and abundant carbon source for industrial microbiology journal, January 2009

Bioconversion of residual glycerol from biodiesel synthesis into 1,3-propanediol and ethanol by isolated bacteria from environmental consortia journal, March 2012

Anaerobic fermentation of glycerol: a path to economic viability for the biofuels industry journal, June 2007

Anaerobic fermentation of glycerol: a platform for renewable fuels and chemicals journal, January 2013

Chaotropicity: a key factor in product tolerance of biofuel-producing microorganisms journal, June 2015

Fermentation of Glycerol into Ethanol in a Microbial Electrolysis Cell Driven by a Customized Consortium journal, May 2014

Fermentative Utilization of Glycerol by Escherichia coli and Its Implications for the Production of Fuels and Chemicals journal, December 2007

Optimization of bioethanol production from glycerol by Escherichia coli SS1 journal, June 2014

Fermentation of glycerol by Clostridium pasteurianum -- batch and continuous culture studies journal, July 2001

Valorization of Cereal Based Biorefinery Byproducts: Reality and Expectations journal, August 2013

Rhodocyclus purpureus gen. nov. and sp. nov., a Ring-Shaped, Vitamin B12-Requiring Member of the Family Rhodospirillaceae journal, April 1978

Enzymes involved in the anaerobic degradation of ortho -phthalate by the nitrate-reducing bacterium Azoarcus sp. strain PA01 : Anaerobic journal, August 2016

Anaerobic pathways of glycerol dissimilation by Enterobacter agglomerans CNCM 1210: limitations and regulations journal, July 1997

The End of Cheap Oil journal, March 1998

A better global resolution function and a novel iterative stochastic search method for optimization of high-performance liquid chromatographic separation journal, April 2005

Ethanol?diesel fuel blends ?? a review journal, February 2005

Fermentation of glycerol to 1,3-propanediol by Klebsiella and Citrobacter strains journal, May 1990

Optimization of substrate composition for biohydrogen production from buffalo slurry co-fermented with cheese whey and crude glycerol, using microbial mixed culture journal, January 2015

Bioconversion of glycerol to ethanol by a mutant Enterobacter aerogenes journal, January 2012

Genomic Encyclopedia of Bacterial and Archaeal Type Strains, Phase III: the genomes of soil and plant-associated and newly described type strains journal, May 2015

Anaerobic nutrition of saccharomyces cerevisiae. II. Unsaturated fatty and requirement for growth in a defined medium journal, June 1954

Effects of glycerol on the growth, adhesion, and cellulolytic activity of rumen cellulolytic bacteria and anaerobic fungi journal, October 1992

The Plate Debate: Cultivable communities have no utility in contemporary environmental microbial ecology: The plate debate journal, June 2007

Engineering alcohol tolerance in yeast journal, October 2014

Ethanol Tolerance in Bacteria journal, January 1989

Propionic acid fermentation from glycerol: comparison with conventional substrates journal, April 1997

Bacterial production of methylglyoxal: a survival strategy or death by misadventure? journal, December 2003

A rapid procedure for the purification of ferredoxin from clostridia using polyethyleneimine journal, May 1978

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding journal, May 1976

Hydrogen production from glycerol by Escherichia coli and other bacteria: An overview and perspectives journal, October 2015

Anaerobium acetethylicum gen. nov., sp. nov., a strictly anaerobic, gluconate-fermenting bacterium isolated from a methanogenic bioreactor journal, October 2015

Metabolic engineering of Escherichia coli for the production of 1,2-propanediol from glycerol journal, December 2010

Anaerobic fermentation of glycerol byEscherichia coli: A new platform for metabolic engineering journal, January 2006

1,3-propanediol production by fermentation: An interesting way to valorize glycerin from the ester and ethanol industries journal, January 1998

Effects of Acetate and Butyrate During Glycerol Fermentation by Clostridium butyricum journal, August 2001

Ethanol-induced water stress and fungal growth journal, January 1998

Anaerobic Fermentation of Glycerol in Paenibacillus macerans: Metabolic Pathways and Environmental Determinants journal, July 2009

96. Acid-catalysed hydration of acraldehyde. Kinetics of the reaction and isolation of β-hydroxypropaldehyde journal, January 1950

Energetics of inter. and intramolecular bonds in alkanediols. iv. the thermochemical study of 1,2-alkanediols at 298.15 K journal, July 1990

Glycerol-Based Biorefinery for Fuels and Chemicals journal, November 2008

Glycerol uptake in Escherichia coli is sensitive to membrane lipid composition journal, January 1993

Recent advances on the production and utilization trends of bio-fuels: A global perspective journal, September 2006

Comparison of the energetic efficiencies of hydrogen and oxychemicals formation in Klebsiella pneumoniae and Clostridium butyricum during anaerobic growth on glycerol journal, April 1995

The biology of Zymomonas. journal, January 1977

IMG ER: a system for microbial genome annotation expert review and curation journal, June 2009

Microbial hydrogen production by bioconversion of crude glycerol: A review journal, April 2012

Optimization of cultural conditions for conversion of glycerol to ethanol by Enterobacter aerogenes S012
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Ethanol Tolerance in the Yeast Saccharomyces cerevisiae Is Dependent on Cellular Oleic Acid Content
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Bioconversion of crude glycerol by fungi
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The Genomes On Line Database (GOLD) in 2007: status of genomic and metagenomic projects and their associated metadata
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Glycerol facilitator of Escherichia coli: cloning of glpF and identification of the glpF product.
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Glycerol: A promising and abundant carbon source for industrial microbiology
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Bioconversion of residual glycerol from biodiesel synthesis into 1,3-propanediol and ethanol by isolated bacteria from environmental consortia
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Anaerobic fermentation of glycerol: a path to economic viability for the biofuels industry
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Anaerobic fermentation of glycerol: a platform for renewable fuels and chemicals
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Chaotropicity: a key factor in product tolerance of biofuel-producing microorganisms
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Fermentation of Glycerol into Ethanol in a Microbial Electrolysis Cell Driven by a Customized Consortium
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Fermentative Utilization of Glycerol by Escherichia coli and Its Implications for the Production of Fuels and Chemicals
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Optimization of bioethanol production from glycerol by Escherichia coli SS1
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Fermentation of glycerol by Clostridium pasteurianum -- batch and continuous culture studies
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Valorization of Cereal Based Biorefinery Byproducts: Reality and Expectations
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Rhodocyclus purpureus gen. nov. and sp. nov., a Ring-Shaped, Vitamin B12-Requiring Member of the Family Rhodospirillaceae
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Enzymes involved in the anaerobic degradation of ortho -phthalate by the nitrate-reducing bacterium Azoarcus sp. strain PA01 : Anaerobic
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Anaerobic pathways of glycerol dissimilation by Enterobacter agglomerans CNCM 1210: limitations and regulations
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The End of Cheap Oil
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A better global resolution function and a novel iterative stochastic search method for optimization of high-performance liquid chromatographic separation
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Ethanol?diesel fuel blends ?? a review
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Fermentation of glycerol to 1,3-propanediol by Klebsiella and Citrobacter strains
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Optimization of substrate composition for biohydrogen production from buffalo slurry co-fermented with cheese whey and crude glycerol, using microbial mixed culture
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Bioconversion of glycerol to ethanol by a mutant Enterobacter aerogenes
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Genomic Encyclopedia of Bacterial and Archaeal Type Strains, Phase III: the genomes of soil and plant-associated and newly described type strains
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Anaerobic nutrition of saccharomyces cerevisiae. II. Unsaturated fatty and requirement for growth in a defined medium
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Effects of glycerol on the growth, adhesion, and cellulolytic activity of rumen cellulolytic bacteria and anaerobic fungi
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The Plate Debate: Cultivable communities have no utility in contemporary environmental microbial ecology: The plate debate
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Engineering alcohol tolerance in yeast
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Ethanol Tolerance in Bacteria
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Propionic acid fermentation from glycerol: comparison with conventional substrates
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Bacterial production of methylglyoxal: a survival strategy or death by misadventure?
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A rapid procedure for the purification of ferredoxin from clostridia using polyethyleneimine
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
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Hydrogen production from glycerol by Escherichia coli and other bacteria: An overview and perspectives
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Anaerobium acetethylicum gen. nov., sp. nov., a strictly anaerobic, gluconate-fermenting bacterium isolated from a methanogenic bioreactor
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Metabolic engineering of Escherichia coli for the production of 1,2-propanediol from glycerol
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Anaerobic fermentation of glycerol byEscherichia coli: A new platform for metabolic engineering
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1,3-propanediol production by fermentation: An interesting way to valorize glycerin from the ester and ethanol industries
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Effects of Acetate and Butyrate During Glycerol Fermentation by Clostridium butyricum
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Ethanol-induced water stress and fungal growth
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Anaerobic Fermentation of Glycerol in Paenibacillus macerans: Metabolic Pathways and Environmental Determinants
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96. Acid-catalysed hydration of acraldehyde. Kinetics of the reaction and isolation of β-hydroxypropaldehyde
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Energetics of inter. and intramolecular bonds in alkanediols. iv. the thermochemical study of 1,2-alkanediols at 298.15 K
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Glycerol-Based Biorefinery for Fuels and Chemicals
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Glycerol uptake in Escherichia coli is sensitive to membrane lipid composition
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Recent advances on the production and utilization trends of bio-fuels: A global perspective
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Comparison of the energetic efficiencies of hydrogen and oxychemicals formation in Klebsiella pneumoniae and Clostridium butyricum during anaerobic growth on glycerol
journal, April 1995

The biology of Zymomonas.
journal, January 1977

IMG ER: a system for microbial genome annotation expert review and curation
journal, June 2009

Microbial hydrogen production by bioconversion of crude glycerol: A review
journal, April 2012