Multiple product inhibition and growth modeling of Clostridium butyricum and Klebsiella pneumoniae in glycerol fermentation

Journal Article · Sat Oct 01 00:00:00 EDT 1994 · Biotechnology and Bioengineering; (United States)

DOI:https://doi.org/10.1002/bit.260440806· OSTI ID:7162388

Zeng, A P; Ross, A; Biebl, H; Tag, C; Guenzel, B; Deckwer, W D ^[1]

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1. Gesellschaft fuer Biotechnologische Forschung mbH, Braunschweig (Germany). Biochemical Engineering Division

The inhibition potentials of products and substrate on the growth of Clostridium butyricum and Klebsiella pneumoniae in the glycerol fermentation are examined from experimental data and with a mathematical model. Whereas the inhibition potential of externally added and self-produced 1,3-propanediol is essentially the same, butyric acid produced by the culture is more toxic than that externally added. The same seems to apply for acetic acid. The inhibitory effect of butyric acid is due to the total concentration instead of its undissociated form. For acetic acid, it cannot be distinguished between the total concentration and the undissociated form. The inhibition effects of products and substrate in the glycerol fermentation are irrespective of the strains, and, therefore, the same growth model can be used. The maximum product concentrations tolerated are 0.35 g/L for undissociated acetic acid, 10.1 g/L for total butyric acid, 16.6 g/L for ethanol, 71.4 g/L for 1,3-propanediol, and 187.6 g/L for glycerol, which are applicable to C. butyricum and K. pneumoniae growth under a variety of conditions. For 55 steady-states, which were obtained from different types of continuous cultures over a pH range of 5.3--8.5 and under both substrate limitation and substrate excess, the proposed growth model fits the experimental data with an average deviation of 17.0%. The deviation of model description from experimental values reduces of 11.4% if only the steady-states with excessive substrate are considered.

• OSTI ID: 7162388
• Journal Information: Biotechnology and Bioengineering; (United States), Vol. 44:8; ISSN 0006-3592
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
ACETIC ACID
BIOLOGICAL EFFECTS
BUTYRIC ACID
CLOSTRIDIUM BUTYRICUM
GROWTH
ETHANOL
BIOSYNTHESIS
GLYCEROL
GLYCOLS
KLEBSIELLA
ALCOHOLS
BACTERIA
CARBOXYLIC ACIDS
CLOSTRIDIUM
HYDROXY COMPOUNDS
MICROORGANISMS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
SYNTHESIS
090900* - Biomass Fuels- Processing- (1990-)