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Title: Transgenic soybean overexpressing GmSAMT1 exhibits resistance to multiple-HG types of soybean cyst nematode Heterodera glycines

Abstract

Soybean (Glycine max (L.) Merr.) salicylic acid methyl transferase (GmSAMT1) catalyses the conversion of salicylic acid to methyl salicylate. Prior results showed that when GmSAMT1 was overexpressed in transgenic soybean hairy roots, resistance is conferred against soybean cyst nematode (SCN), Heterodera glycines Ichinohe. In this study, we produced transgenic soybean overexpressing GmSAMT1 and characterized their response to various SCN races. Transgenic plants conferred a significant reduction in the development of SCN HG type 1.2.5.7 (race 2), HG type 0 (race 3) and HG type 2.5.7 (race 5). Among transgenic lines, GmSAMT1 expression in roots was positively associated with SCN resistance. In some transgenic lines, there was a significant decrease in salicylic acid titer relative to control plants. No significant seed yield differences were observed between transgenics and control soybean plants grown in one greenhouse with 22 °C day/night temperature, whereas transgenic soybean had higher yield than controls grown a warmer greenhouse (27 °C day/23 °C night) temperature. In a 1-year field experiment in Knoxville, TN, there was no significant difference in seed yield between the transgenic and nontransgenic soybean under conditions with negligible SCN infection. We hypothesize that GmSAMT1 expression affects salicylic acid biosynthesis, which, in turn, attenuates SCN development,more » without negative consequences to soybean yield or other morphological traits. Furthermore, we conclude that GmSAMT1 overexpression confers broad resistance to multiple SCN races, which would be potentially applicable to commercial production.« less

Authors:
 [1];  [1];  [2];  [1];  [1];  [1];  [2];  [1];  [3];  [1];  [1];  [1]
+ Show Author Affiliations
  1. Univ. of Tennessee, Knoxville, TN (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  3. USDA-ARS, Jackson, TN (United States)
Publication Date:
Research Org.:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1329152
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Plant Biotechnology Journal
Additional Journal Information:
Journal Volume: 14; Journal Issue: 11; Journal ID: ISSN 1467-7644
Publisher:
Society for Experimental Biology; Association of Applied Biology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; salycic acid methyltransferase; transgenic soybean; soybean cyst nematode; gene expression; metabolite; female index; yield

Citation Formats

Lin, Jingyu, Mazarei, Mitra, Zhao, Nan, Hatcher, Catherine N., Wuddineh, Wegi A., Rudis, Mary, Tschaplinski, Timothy J., Pantalone, Vincent R., Arelli, Prakash R., Hewezi, Tarek, Chen, Feng, and Stewart, Jr., Charles Neal. Transgenic soybean overexpressing GmSAMT1 exhibits resistance to multiple-HG types of soybean cyst nematode Heterodera glycines. United States: N. p., 2016. Web. doi:10.1111/pbi.12566.
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Lin, Jingyu, Mazarei, Mitra, Zhao, Nan, Hatcher, Catherine N., Wuddineh, Wegi A., Rudis, Mary, Tschaplinski, Timothy J., Pantalone, Vincent R., Arelli, Prakash R., Hewezi, Tarek, Chen, Feng, & Stewart, Jr., Charles Neal. Transgenic soybean overexpressing GmSAMT1 exhibits resistance to multiple-HG types of soybean cyst nematode Heterodera glycines. United States. https://doi.org/10.1111/pbi.12566
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Lin, Jingyu, Mazarei, Mitra, Zhao, Nan, Hatcher, Catherine N., Wuddineh, Wegi A., Rudis, Mary, Tschaplinski, Timothy J., Pantalone, Vincent R., Arelli, Prakash R., Hewezi, Tarek, Chen, Feng, and Stewart, Jr., Charles Neal. Mon . "Transgenic soybean overexpressing GmSAMT1 exhibits resistance to multiple-HG types of soybean cyst nematode Heterodera glycines". United States. https://doi.org/10.1111/pbi.12566. https://www.osti.gov/servlets/purl/1329152.
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@article{osti_1329152,
title = {Transgenic soybean overexpressing GmSAMT1 exhibits resistance to multiple-HG types of soybean cyst nematode Heterodera glycines},
author = {Lin, Jingyu and Mazarei, Mitra and Zhao, Nan and Hatcher, Catherine N. and Wuddineh, Wegi A. and Rudis, Mary and Tschaplinski, Timothy J. and Pantalone, Vincent R. and Arelli, Prakash R. and Hewezi, Tarek and Chen, Feng and Stewart, Jr., Charles Neal},
abstractNote = {Soybean (Glycine max (L.) Merr.) salicylic acid methyl transferase (GmSAMT1) catalyses the conversion of salicylic acid to methyl salicylate. Prior results showed that when GmSAMT1 was overexpressed in transgenic soybean hairy roots, resistance is conferred against soybean cyst nematode (SCN), Heterodera glycines Ichinohe. In this study, we produced transgenic soybean overexpressing GmSAMT1 and characterized their response to various SCN races. Transgenic plants conferred a significant reduction in the development of SCN HG type 1.2.5.7 (race 2), HG type 0 (race 3) and HG type 2.5.7 (race 5). Among transgenic lines, GmSAMT1 expression in roots was positively associated with SCN resistance. In some transgenic lines, there was a significant decrease in salicylic acid titer relative to control plants. No significant seed yield differences were observed between transgenics and control soybean plants grown in one greenhouse with 22 °C day/night temperature, whereas transgenic soybean had higher yield than controls grown a warmer greenhouse (27 °C day/23 °C night) temperature. In a 1-year field experiment in Knoxville, TN, there was no significant difference in seed yield between the transgenic and nontransgenic soybean under conditions with negligible SCN infection. We hypothesize that GmSAMT1 expression affects salicylic acid biosynthesis, which, in turn, attenuates SCN development, without negative consequences to soybean yield or other morphological traits. Furthermore, we conclude that GmSAMT1 overexpression confers broad resistance to multiple SCN races, which would be potentially applicable to commercial production.},
doi = {10.1111/pbi.12566},
journal = {Plant Biotechnology Journal},
number = 11,
volume = 14,
place = {United States},
year = {Mon May 23 00:00:00 EDT 2016},
month = {Mon May 23 00:00:00 EDT 2016}
}
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https://doi.org/10.1111/pbi.12566
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    Works referencing / citing this record:

    Comparative RNA-Seq Analysis Uncovers a Complex Regulatory Network for Soybean Cyst Nematode Resistance in Wild Soybean (Glycine soja)
    journal, August 2017

    An ( E,E )-α-farnesene synthase gene of soybean has a role in defence against nematodes and is involved in synthesizing insect-induced volatiles
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    • DOI: 10.1111/pbi.12649

    Comparative RNA-Seq Analysis Uncovers a Complex Regulatory Network for Soybean Cyst Nematode Resistance in Wild Soybean (Glycine soja)
    text, January 2017

    • Yan, Luo,; Hengyou, Zhang,; Susanne, Kjemtrup-Lovelace,
    • The University of North Carolina at Chapel Hill University Libraries
    • DOI: 10.17615/2kg0-ae18
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