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Title: Expression of cyanobacterial FBP/SBPase in soybean prevents yield depression under future climate conditions

Abstract

Predictions suggest that current crop production needs to double by 2050 to meet global food and energy demands. Based on theory and experimental studies, overexpression of the photosynthetic enzyme sedoheptulose-1,7-bisphosphatase (SBPase) is expected to enhance C3 crop photosynthesis and yields. Here we test how expression of the cyanobacterial, bifunctional fructose-1,6/sedoheptulose-1,7-bisphosphatase (FBP/SBPase) affects carbon assimilation and seed yield (SY) in a major crop (soybean, Glycine max). For three growing seasons, wild-type (WT) and FBP/SBPase-expressing (FS) plants were grown in the field under ambient (400 μmol mol-1) and elevated (600 μmol mol-1) CO2 concentrations [CO2] and under ambient and elevated temperatures (+2.7 °C during daytime, +3.4 °C at night) at the SoyFACE research site. Across treatments, FS plants had significantly higher carbon assimilation (4–14%), Vc,max (5–8%), and Jmax (4–8%). Under ambient [CO2], elevated temperature led to significant reductions of SY of both genotypes by 19–31%. However, under elevated [CO2] and elevated temperature, FS plants maintained SY levels, while the WT showed significant reductions between 11% and 22% compared with plants under elevated [CO2] alone. These results show that the manipulation of the photosynthetic carbon reduction cycle can mitigate the effects of future high CO2 and high temperature environments on soybean yield.

Authors:
 [1];  [2]; ORCiD logo [3];  [4];  [5]; ORCiD logo [6];  [7]; ORCiD logo [7]
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  1. United States Department of Agriculture, Urbana, IL (United States). Global Change and Photosynthesis Research Unit, Agricultural Research Service; University of Illinois at Urbana-Champaign, Urbana, IL (United States). Carl R. Woese Institute for Genomic Biology
  2. University of Illinois at Urbana-Champaign, Urbana, IL (United States). Carl R. Woese Institute for Genomic Biology
  3. Iowa State University, Ames, IA (United States). Department of Agronomy
  4. United States Department of Agriculture, Urbana, IL (United States). Global Change and Photosynthesis Research Unit, Agricultural Research Service; University of Illinois at Urbana-Champaign, Urbana, IL (United States). Carl R. Woese Institute for Genomic Biology
  5. University of Nebraska-Lincoln, Lincoln, NE (United States). Center for Plant Science Innovation
  6. United States Department of Agriculture, Urbana, IL (United States). Global Change and Photosynthesis Research Unit, Agricultural Research Service; University of Illinois at Urbana-Champaign, Urbana, IL (United States). Carl R. Woese Institute for Genomic Biology; University of Lancaster, Lancaster (United Kingdom). Lancaster Environment Centre
  7. United States Department of Agriculture, Urbana, IL (United States). Global Change and Photosynthesis Research Unit, Agricultural Research Service; University of Illinois at Urbana-Champaign, Urbana, IL (United States). Carl R. Woese Institute for Genomic Biology; University of Illinois at Urbana-Champaign, Urbana, IL (United States). Departments of Plant Biology and Crop Sciences
Publication Date:
Research Org.:
Oak Ridge Associated Univ., Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1625388
Grant/Contract Number:  
AC05-06OR23100
Resource Type:
Accepted Manuscript
Journal Name:
Journal of Experimental Botany
Additional Journal Information:
Journal Volume: 68; Journal Issue: 3; Journal ID: ISSN 0022-0957
Publisher:
Oxford University Press
Country of Publication:
United States
Language:
English
Subject:
Plant Sciences

Citation Formats

Köhler, Iris H., Ruiz-Vera, Ursula M., VanLoocke, Andy, Thomey, Michell L., Clemente, Tom, Long, Stephen P., Ort, Donald R., and Bernacchi, Carl J. Expression of cyanobacterial FBP/SBPase in soybean prevents yield depression under future climate conditions. United States: N. p., 2016. Web. doi:10.1093/jxb/erw435.
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Köhler, Iris H., Ruiz-Vera, Ursula M., VanLoocke, Andy, Thomey, Michell L., Clemente, Tom, Long, Stephen P., Ort, Donald R., & Bernacchi, Carl J. Expression of cyanobacterial FBP/SBPase in soybean prevents yield depression under future climate conditions. United States. https://doi.org/10.1093/jxb/erw435
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Köhler, Iris H., Ruiz-Vera, Ursula M., VanLoocke, Andy, Thomey, Michell L., Clemente, Tom, Long, Stephen P., Ort, Donald R., and Bernacchi, Carl J. Mon . "Expression of cyanobacterial FBP/SBPase in soybean prevents yield depression under future climate conditions". United States. https://doi.org/10.1093/jxb/erw435. https://www.osti.gov/servlets/purl/1625388.
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@article{osti_1625388,
title = {Expression of cyanobacterial FBP/SBPase in soybean prevents yield depression under future climate conditions},
author = {Köhler, Iris H. and Ruiz-Vera, Ursula M. and VanLoocke, Andy and Thomey, Michell L. and Clemente, Tom and Long, Stephen P. and Ort, Donald R. and Bernacchi, Carl J.},
abstractNote = {Predictions suggest that current crop production needs to double by 2050 to meet global food and energy demands. Based on theory and experimental studies, overexpression of the photosynthetic enzyme sedoheptulose-1,7-bisphosphatase (SBPase) is expected to enhance C3 crop photosynthesis and yields. Here we test how expression of the cyanobacterial, bifunctional fructose-1,6/sedoheptulose-1,7-bisphosphatase (FBP/SBPase) affects carbon assimilation and seed yield (SY) in a major crop (soybean, Glycine max). For three growing seasons, wild-type (WT) and FBP/SBPase-expressing (FS) plants were grown in the field under ambient (400 μmol mol-1) and elevated (600 μmol mol-1) CO2 concentrations [CO2] and under ambient and elevated temperatures (+2.7 °C during daytime, +3.4 °C at night) at the SoyFACE research site. Across treatments, FS plants had significantly higher carbon assimilation (4–14%), Vc,max (5–8%), and Jmax (4–8%). Under ambient [CO2], elevated temperature led to significant reductions of SY of both genotypes by 19–31%. However, under elevated [CO2] and elevated temperature, FS plants maintained SY levels, while the WT showed significant reductions between 11% and 22% compared with plants under elevated [CO2] alone. These results show that the manipulation of the photosynthetic carbon reduction cycle can mitigate the effects of future high CO2 and high temperature environments on soybean yield.},
doi = {10.1093/jxb/erw435},
journal = {Journal of Experimental Botany},
number = 3,
volume = 68,
place = {United States},
year = {Mon Dec 12 00:00:00 EST 2016},
month = {Mon Dec 12 00:00:00 EST 2016}
}
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Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1093/jxb/erw435
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Figures / Tables:

Table 1 Table 1: Planting and harvest dates and climatic parameters in the study years. Canopy temperature (in °C) is averaged for the time period from canopy closure (V5, fifth node stage) to R7 (beginning of maturity) developmental stages (Ritchie et al., 1993) on the control (c) and heated (h) plotsmore » under ambient (400 $μ$mol m−2 s−1) and elevated (600 $μ$mol m−2 s−1) [CO2] with periods of rain excluded for the calculations. $Δ$Canopy temperature (in °C) is the difference between heated and control plots within each CO2 treatment. Day is averaged from 6:00 h to 18:00 h and night from 18:00 h to 6:00 h.« less
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Increasing Photosynthetic Carbon Assimilation in C 3 Plants to Improve Crop Yield: Current and Future Strategies
journal, November 2010

Global Warming Can Negate the Expected CO 2 Stimulation in Photosynthesis and Productivity for Soybean Grown in the Midwestern United States
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    Works referencing / citing this record:

    Introgression of UfCyt c6, a thylakoid lumen protein from a green seaweed Ulva fasciata Delile enhanced photosynthesis and growth in tobacco
    journal, August 2018

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    Engineering photosynthesis: progress and perspectives
    journal, January 2017

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    • Journal of Experimental Botany, Vol. 70, Issue 4
    • DOI: 10.1093/jxb/ery445

    The influence of rising tropospheric carbon dioxide and ozone on plant productivity
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    Engineering photosynthesis: progress and perspectives
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    Photosynthesis in a Changing Global Climate: Scaling Up and Scaling Down in Crops
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    • Baslam, Marouane; Mitsui, Toshiaki; Hodges, Michael
    • Frontiers in Plant Science, Vol. 11
    • DOI: 10.3389/fpls.2020.00882
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