Difference in C3–C4 metabolism underlies tradeoff between growth rate and biomass yield in Methylobacterium extorquens AM1
Abstract
In this study, two variants of Methylobacterium extorquens AM1 demonstrated a trade-off between growth rate and biomass yield. In addition, growth rate and biomass yield were also affected by supplementation of growth medium with different amounts of cobalt. The metabolism changes relating to these growth phenomena as well as the trade-off were investigated in this study. 13C metabolic flux analysis was used to generate a detailed central carbon metabolic flux map with both absolute and normalized flux values. As a result, the major differences between the two variants occurred at the formate node as well as within C3-C4 inter-conversion pathways. Higher relative fluxes through formyltetrahydrofolate ligase, phosphoenolpyruvate carboxylase, and malic enzyme led to higher biomass yield, while higher relative fluxes through pyruvate kinase and pyruvate dehydrogenase led to higher growth rate. These results were then tested by phenotypic studies on three mutants (null pyk, null pck mutant and null dme mutant) in both variants, which agreed with the model prediction. In this study, 13C metabolic flux analysis for two strain variants of M. extorquens AM1 successfully identified metabolic pathways contributing to the trade-off between cell growth and biomass yield. Phenotypic analysis of mutants deficient in corresponding genes supported the conclusionmore »
- Authors:
- Publication Date:
- Research Org.:
- Univ. of Washington, Seattle, WA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1618591
- Alternate Identifier(s):
- OSTI ID: 1299217
- Grant/Contract Number:
- SC0006871
- Resource Type:
- Published Article
- Journal Name:
- BMC Microbiology
- Additional Journal Information:
- Journal Name: BMC Microbiology Journal Volume: 16 Journal Issue: 1; Journal ID: ISSN 1471-2180
- Publisher:
- Springer Science + Business Media
- Country of Publication:
- United Kingdom
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Methylobacterium extorquens AM1; Methylotrophy; physiological trade-off; metabolic flux analysis; cobalt; flux analysis; labeling experiments; reconstruction; methanol; formate; point
Citation Formats
Fu, Yanfen, Beck, David A. C., and Lidstrom, Mary E. Difference in C3–C4 metabolism underlies tradeoff between growth rate and biomass yield in Methylobacterium extorquens AM1. United Kingdom: N. p., 2016.
Web. doi:10.1186/s12866-016-0778-4.
Fu, Yanfen, Beck, David A. C., & Lidstrom, Mary E. Difference in C3–C4 metabolism underlies tradeoff between growth rate and biomass yield in Methylobacterium extorquens AM1. United Kingdom. https://doi.org/10.1186/s12866-016-0778-4
Fu, Yanfen, Beck, David A. C., and Lidstrom, Mary E. Tue .
"Difference in C3–C4 metabolism underlies tradeoff between growth rate and biomass yield in Methylobacterium extorquens AM1". United Kingdom. https://doi.org/10.1186/s12866-016-0778-4.
@article{osti_1618591,
title = {Difference in C3–C4 metabolism underlies tradeoff between growth rate and biomass yield in Methylobacterium extorquens AM1},
author = {Fu, Yanfen and Beck, David A. C. and Lidstrom, Mary E.},
abstractNote = {In this study, two variants of Methylobacterium extorquens AM1 demonstrated a trade-off between growth rate and biomass yield. In addition, growth rate and biomass yield were also affected by supplementation of growth medium with different amounts of cobalt. The metabolism changes relating to these growth phenomena as well as the trade-off were investigated in this study. 13C metabolic flux analysis was used to generate a detailed central carbon metabolic flux map with both absolute and normalized flux values. As a result, the major differences between the two variants occurred at the formate node as well as within C3-C4 inter-conversion pathways. Higher relative fluxes through formyltetrahydrofolate ligase, phosphoenolpyruvate carboxylase, and malic enzyme led to higher biomass yield, while higher relative fluxes through pyruvate kinase and pyruvate dehydrogenase led to higher growth rate. These results were then tested by phenotypic studies on three mutants (null pyk, null pck mutant and null dme mutant) in both variants, which agreed with the model prediction. In this study, 13C metabolic flux analysis for two strain variants of M. extorquens AM1 successfully identified metabolic pathways contributing to the trade-off between cell growth and biomass yield. Phenotypic analysis of mutants deficient in corresponding genes supported the conclusion that C3-C4 inter-conversion strategies were the major response to the trade-off.},
doi = {10.1186/s12866-016-0778-4},
journal = {BMC Microbiology},
number = 1,
volume = 16,
place = {United Kingdom},
year = {Tue Jul 19 00:00:00 EDT 2016},
month = {Tue Jul 19 00:00:00 EDT 2016}
}
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https://doi.org/10.1186/s12866-016-0778-4
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Cited by: 10 works
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Figures / Tables:
Fig. 1: Central carbon metabolism model for M. extorquens AM1 methylotrophic growth. Metabolites with * are precursors for biomass. Metabolites in bold are branch points. The model includes 114 reactions with 9 reversible reactions and 2 scramble reactions. 71 intracellular metabolites are included in the model. Methanol is oxidized tomore »
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A pathway for biological methane production using bacterial iron-only nitrogenase
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Figures / Tables found in this record:
Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.