Effect of aging on lignin content, composition and enzymatic saccharification in Corymbia hybrids and parental taxa between years 9 and 12
Abstract
Corymbia (a eucalypt) is an important forestry genus and a potential lignocellulosic bioenergy feedstock. The composition of the lignocellulosic cell wall significantly impacts pretreatment efficiency and conversion to biofuel but is variable and changes with age. In this study, we estimated Klason lignin content, composition, and monosaccharide (glucose and xylose) release after enzymatic saccharification of untreated and hydrothermally pretreated biomass from Corymbia parental species Corymbia torelliana (CT), Corymbia citriodora subsp. variegata (spotted gum; CCV), and interspecific F1 hybrids (CT × CCV) at ages 9 and 12 years from planting. Analysis of lignin composition derived from syringyl/guaiacyl monolignols (S/G) found significant differences among taxa, with CT S/G ratios (2.2 and 2.0) being significantly lower than CCV (2.6 and 2.3) or hybrids (2.5 and 2.3) at ages 9 and 12 respectively. In general, enzymatic saccharification yields from untreated biomass were significantly different among taxa, with CT (113 and 75 mg g -1 ) and hybrids (108 and 81 mg g -1 ) yielding significantly higher glucose from untreated biomass than CCV (82 and 56 mg g -1 ) at ages 9 and 12 respectively. Comparison of traits within taxa between ages 9 and 12 found S/G ratios and glucose yields from untreated biomass were significantly lower in CT, CCV andmore »
- Authors:
-
[3];
- Univ. of Queensland, Queensland (Australia)
- Sage Analytics, Boulder, CO (United States)
- Univ. of Sunshine Coast, Queensland (Australia); Agri-Science Queensland, Gympie (Australia)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Sandia National Lab. (SNL-CA), Livermore, CA (United States)
- Univ. of Queensland, Queensland (Australia); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Sandia National Lab. (SNL-CA), Livermore, CA (United States)
- Publication Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1266697
- Alternate Identifier(s):
- OSTI ID: 1335178; OSTI ID: 1440941
- Report Number(s):
- NREL/JA-2700-64939
Journal ID: ISSN 0961-9534
- Grant/Contract Number:
- AC36-08GO28308; AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Biomass and Bioenergy
- Additional Journal Information:
- Journal Volume: 93; Journal Issue: C; Journal ID: ISSN 0961-9534
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; Corymbia; saccharification; lignin; S/G; bioproducts; biofuels
Citation Formats
Healey, Adam L., Lupoi, Jason S., Lee, David J., Sykes, Robert W., Guenther, Joel M., Tran, Kim, Decker, Stephen R., Singh, Seema, Simmons, Blake A., and Henry, Robert J. Effect of aging on lignin content, composition and enzymatic saccharification in Corymbia hybrids and parental taxa between years 9 and 12. United States: N. p., 2016.
Web. doi:10.1016/j.biombioe.2016.06.016.
Healey, Adam L., Lupoi, Jason S., Lee, David J., Sykes, Robert W., Guenther, Joel M., Tran, Kim, Decker, Stephen R., Singh, Seema, Simmons, Blake A., & Henry, Robert J. Effect of aging on lignin content, composition and enzymatic saccharification in Corymbia hybrids and parental taxa between years 9 and 12. United States. https://doi.org/10.1016/j.biombioe.2016.06.016
Healey, Adam L., Lupoi, Jason S., Lee, David J., Sykes, Robert W., Guenther, Joel M., Tran, Kim, Decker, Stephen R., Singh, Seema, Simmons, Blake A., and Henry, Robert J. Sat .
"Effect of aging on lignin content, composition and enzymatic saccharification in Corymbia hybrids and parental taxa between years 9 and 12". United States. https://doi.org/10.1016/j.biombioe.2016.06.016. https://www.osti.gov/servlets/purl/1266697.
@article{osti_1266697,
title = {Effect of aging on lignin content, composition and enzymatic saccharification in Corymbia hybrids and parental taxa between years 9 and 12},
author = {Healey, Adam L. and Lupoi, Jason S. and Lee, David J. and Sykes, Robert W. and Guenther, Joel M. and Tran, Kim and Decker, Stephen R. and Singh, Seema and Simmons, Blake A. and Henry, Robert J.},
abstractNote = {Corymbia (a eucalypt) is an important forestry genus and a potential lignocellulosic bioenergy feedstock. The composition of the lignocellulosic cell wall significantly impacts pretreatment efficiency and conversion to biofuel but is variable and changes with age. In this study, we estimated Klason lignin content, composition, and monosaccharide (glucose and xylose) release after enzymatic saccharification of untreated and hydrothermally pretreated biomass from Corymbia parental species Corymbia torelliana (CT), Corymbia citriodora subsp. variegata (spotted gum; CCV), and interspecific F1 hybrids (CT × CCV) at ages 9 and 12 years from planting. Analysis of lignin composition derived from syringyl/guaiacyl monolignols (S/G) found significant differences among taxa, with CT S/G ratios (2.2 and 2.0) being significantly lower than CCV (2.6 and 2.3) or hybrids (2.5 and 2.3) at ages 9 and 12 respectively. In general, enzymatic saccharification yields from untreated biomass were significantly different among taxa, with CT (113 and 75 mg g -1 ) and hybrids (108 and 81 mg g -1 ) yielding significantly higher glucose from untreated biomass than CCV (82 and 56 mg g -1 ) at ages 9 and 12 respectively. Comparison of traits within taxa between ages 9 and 12 found S/G ratios and glucose yields from untreated biomass were significantly lower in CT, CCV and hybrid taxa. In conclusion, the formation of lignocellulosic cell walls is complex, influenced by genetics and age of material, requiring optimization of rotation age for biofuel production and other industrial processes.},
doi = {10.1016/j.biombioe.2016.06.016},
journal = {Biomass and Bioenergy},
number = C,
volume = 93,
place = {United States},
year = {Sat Jul 02 00:00:00 EDT 2016},
month = {Sat Jul 02 00:00:00 EDT 2016}
}
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