Effect of lignin content on changes occurring in poplar cellulose ultrastructure during dilute acid pretreatment
Abstract
Obtaining a better understanding of the complex mechanisms occurring during lignocellulosic deconstruction is critical to the continued growth of renewable biofuel production. A key step in bioethanol production is thermochemical pretreatment to reduce plant cell wall recalcitrance for downstream processes. Previous studies of dilute acid pretreatment (DAP) have shown significant changes in cellulose ultrastructure that occur during pretreatment, but there is still a substantial knowledge gap with respect to the influence of lignin on these cellulose ultrastructural changes. This study was designed to assess how the presence of lignin influences DAP-induced changes in cellulose ultrastructure, which might ultimately have large implications with respect to enzymatic deconstruction efforts. Native, untreated hybrid poplar (Populus trichocarpa x Populus deltoids) samples and a partially delignified poplar sample (facilitated by acidic sodium chlorite pulping) were separately pretreated with dilute sulfuric acid (0.10 M) at 160°C for 15 minutes and 35 minutes, respectively . Following extensive characterization, the partially delignified biomass displayed more significant changes in cellulose ultrastructure following DAP than the native untreated biomass. With respect to the native untreated poplar, delignified poplar after DAP (in which approximately 40% lignin removal occurred) experienced: increased cellulose accessibility indicated by increased Simons’ stain (orange dye) adsorption frommore »
- Authors:
-
- Georgia Inst. of Technology, Atlanta, GA (United States). School of Chemistry and Biochemistry
- Washington Univ., St. Louis, MO (United States)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Structural Molecular Biology (CSMB)
- Univ. of California, Riverside, CA (United States). Bourns College of Engineering
- Univ. of California, Riverside, CA (United States). Bourns College of Engineering; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
- Georgia Inst. of Technology, Atlanta, GA (United States). School of Chemistry and Biochemistry; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Univ. of Tennessee, Knoxville, TN (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1259860
- Grant/Contract Number:
- FWP ERKP752; FWP ERKP291
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Biotechnology for Biofuels
- Additional Journal Information:
- Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 1754-6834
- Publisher:
- BioMed Central
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; Cellulose ultrastructure; Lignin content; Dilute acid pretreatment; Delignification; Enzymatic sugar release; Biomass recalcitrance
Citation Formats
Sun, Qining, Foston, Marcus, Meng, Xianzhi, Sawada, Daisuke, Pingali, Sai Venkatesh, O’Neill, Hugh M., Li, Hongjia, Wyman, Charles E., Langan, Paul, Ragauskas, Art J., and Kumar, Rajeev. Effect of lignin content on changes occurring in poplar cellulose ultrastructure during dilute acid pretreatment. United States: N. p., 2014.
Web. doi:10.1186/s13068-014-0150-6.
Sun, Qining, Foston, Marcus, Meng, Xianzhi, Sawada, Daisuke, Pingali, Sai Venkatesh, O’Neill, Hugh M., Li, Hongjia, Wyman, Charles E., Langan, Paul, Ragauskas, Art J., & Kumar, Rajeev. Effect of lignin content on changes occurring in poplar cellulose ultrastructure during dilute acid pretreatment. United States. https://doi.org/10.1186/s13068-014-0150-6
Sun, Qining, Foston, Marcus, Meng, Xianzhi, Sawada, Daisuke, Pingali, Sai Venkatesh, O’Neill, Hugh M., Li, Hongjia, Wyman, Charles E., Langan, Paul, Ragauskas, Art J., and Kumar, Rajeev. Tue .
"Effect of lignin content on changes occurring in poplar cellulose ultrastructure during dilute acid pretreatment". United States. https://doi.org/10.1186/s13068-014-0150-6. https://www.osti.gov/servlets/purl/1259860.
@article{osti_1259860,
title = {Effect of lignin content on changes occurring in poplar cellulose ultrastructure during dilute acid pretreatment},
author = {Sun, Qining and Foston, Marcus and Meng, Xianzhi and Sawada, Daisuke and Pingali, Sai Venkatesh and O’Neill, Hugh M. and Li, Hongjia and Wyman, Charles E. and Langan, Paul and Ragauskas, Art J. and Kumar, Rajeev},
abstractNote = {Obtaining a better understanding of the complex mechanisms occurring during lignocellulosic deconstruction is critical to the continued growth of renewable biofuel production. A key step in bioethanol production is thermochemical pretreatment to reduce plant cell wall recalcitrance for downstream processes. Previous studies of dilute acid pretreatment (DAP) have shown significant changes in cellulose ultrastructure that occur during pretreatment, but there is still a substantial knowledge gap with respect to the influence of lignin on these cellulose ultrastructural changes. This study was designed to assess how the presence of lignin influences DAP-induced changes in cellulose ultrastructure, which might ultimately have large implications with respect to enzymatic deconstruction efforts. Native, untreated hybrid poplar (Populus trichocarpa x Populus deltoids) samples and a partially delignified poplar sample (facilitated by acidic sodium chlorite pulping) were separately pretreated with dilute sulfuric acid (0.10 M) at 160°C for 15 minutes and 35 minutes, respectively . Following extensive characterization, the partially delignified biomass displayed more significant changes in cellulose ultrastructure following DAP than the native untreated biomass. With respect to the native untreated poplar, delignified poplar after DAP (in which approximately 40% lignin removal occurred) experienced: increased cellulose accessibility indicated by increased Simons’ stain (orange dye) adsorption from 21.8 to 72.5 mg/g, decreased cellulose weight-average degree of polymerization (DPw) from 3087 to 294 units, and increased cellulose crystallite size from 2.9 to 4.2 nm. These changes following DAP ultimately increased enzymatic sugar yield from 10 to 80%. We conclude that, overall, the results indicate a strong influence of lignin content on cellulose ultrastructural changes occurring during DAP. With the reduction of lignin content during DAP, the enlargement of cellulose microfibril dimensions and crystallite size becomes more apparent. Further, this enlargement of cellulose microfibril dimensions is attributed to specific processes, including the co-crystallization of crystalline cellulose driven by irreversible inter-chain hydrogen bonding (similar to hornification) and/or cellulose annealing that converts amorphous cellulose to paracrystalline and crystalline cellulose. Essentially, lignin acts as a barrier to prevent cellulose crystallinity increase and cellulose fibril coalescence during DAP.},
doi = {10.1186/s13068-014-0150-6},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 7,
place = {United States},
year = {Tue Oct 14 00:00:00 EDT 2014},
month = {Tue Oct 14 00:00:00 EDT 2014}
}
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Lignin Depletion Enhances the Digestibility of Cellulose in Cultured Xylem Cells
journal, July 2013
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journal, January 2019
- Satari, Behzad; Karimi, Keikhosro; Kumar, Rajeev
- Sustainable Energy & Fuels, Vol. 3, Issue 1
Altered lignocellulose chemical structure and molecular assembly in CINNAMYL ALCOHOL DEHYDROGENASE-deficient rice
journal, November 2019
- Martin, Andri Fadillah; Tobimatsu, Yuki; Kusumi, Ryosuke
- Scientific Reports, Vol. 9, Issue 1
Isolation and Characterization of Novel Lignolytic, Cellulolytic, and Hemicellulolytic Bacteria from Wood-Feeding Termite Cryptotermes brevis
journal, July 2018
- Tsegaye, Bahiru; Balomajumder, Chandrajit; Roy, Partha
- International Microbiology, Vol. 22, Issue 1
Characterization of lignin derived from water-only and dilute acid flowthrough pretreatment of poplar wood at elevated temperatures
journal, December 2015
- Zhang, Libing; Yan, Lishi; Wang, Zheming
- Biotechnology for Biofuels, Vol. 8, Issue 1
Advances in understanding the surface chemistry of lignocellulosic biomass via time-of-flight secondary ion mass spectrometry
journal, December 2016
- Tolbert, Allison; Ragauskas, Arthur J.
- Energy Science & Engineering, Vol. 5, Issue 1
New perspective on glycoside hydrolase binding to lignin from pretreated corn stover
journal, December 2015
- Yarbrough, John M.; Mittal, Ashutosh; Mansfield, Elisabeth
- Biotechnology for Biofuels, Vol. 8, Issue 1
Recent advances in understanding the pseudo-lignin formation in a lignocellulosic biorefinery
journal, January 2018
- Shinde, Somnath D.; Meng, Xianzhi; Kumar, Rajeev
- Green Chemistry, Vol. 20, Issue 10
Saccharification of pretreated sawdust by Aspergillus niger cellulase
journal, March 2015
- Sridevi, A.; Narasimha, G.; Ramanjaneyulu, G.
- 3 Biotech, Vol. 5, Issue 6
Altered lignocellulose chemical structure and molecular assembly in CINNAMYL ALCOHOL DEHYDROGENASE-deficient rice
journal, November 2019
- Martin, Andri Fadillah; Tobimatsu, Yuki; Kusumi, Ryosuke
- Scientific Reports, Vol. 9, Issue 1
Laccase mediated delignification of pineapple leaf waste: an ecofriendly sustainable attempt towards valorization
journal, April 2019
- Banerjee, Rintu; Chintagunta, Anjani Devi; Ray, Subhabrata
- BMC Chemistry, Vol. 13, Issue 1
Characterization of lignin derived from water-only and dilute acid flowthrough pretreatment of poplar wood at elevated temperatures
journal, December 2015
- Zhang, Libing; Yan, Lishi; Wang, Zheming
- Biotechnology for Biofuels, Vol. 8, Issue 1
New perspective on glycoside hydrolase binding to lignin from pretreated corn stover
journal, December 2015
- Yarbrough, John M.; Mittal, Ashutosh; Mansfield, Elisabeth
- Biotechnology for Biofuels, Vol. 8, Issue 1
Consolidated bioprocessing of Populus using Clostridium (Ruminiclostridium) thermocellum: a case study on the impact of lignin composition and structure
journal, February 2016
- Dumitrache, Alexandru; Akinosho, Hannah; Rodriguez, Miguel
- Biotechnology for Biofuels, Vol. 9, Issue 1
Physical and chemical differences between one-stage and two-stage hydrothermal pretreated hardwood substrates for use in cellulosic ethanol production
journal, February 2016
- Guilliams, Andrew; Pattathil, Sivakumar; Willies, Deidre
- Biotechnology for Biofuels, Vol. 9, Issue 1
Production of xylooligosaccharides and monosaccharides from poplar by a two-step acetic acid and peroxide/acetic acid pretreatment
journal, April 2019
- Wen, Peiyao; Zhang, Tian; Wang, Jinye
- Biotechnology for Biofuels, Vol. 12, Issue 1