Rapid estimation of sugar release from winter wheat straw during bioethanol production using FTIR-photoacoustic spectroscopy

Bekiaris, Georgios; Lindedam, Jane; Peltre, Clément; Decker, Stephen R.; Turner, Geoffrey B.; Magid, Jakob; Bruun, Sander

doi:10.1186/s13068-015-0267-2

Title: Rapid estimation of sugar release from winter wheat straw during bioethanol production using FTIR-photoacoustic spectroscopy

Journal Article · Thu Jun 18 00:00:00 EDT 2015 · Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-015-0267-2 · OSTI ID:1259666

Bekiaris, Georgios ^[1]; Lindedam, Jane ^[1]; Peltre, Clément ^[1]; Decker, Stephen R. ^[2]; Turner, Geoffrey B. ^[2]; Magid, Jakob ^[1]; Bruun, Sander ^[1]

Univ. of Copenhagen (Denmark). Dept.of Plant and Environmental Sciences
National Renewable Energy Lab. (NREL), Golden, CO (United States)

Complexity and high cost are the main limitations for high-throughput screening methods for the estimation of the sugar release from plant materials during bioethanol production. In addition, it is important that we improve our understanding of the mechanisms by which different chemical components are affecting the degradability of plant material. In this study, Fourier transform infrared photoacoustic spectroscopy (FTIR-PAS) was combined with advanced chemometrics to develop calibration models predicting the amount of sugars released after pretreatment and enzymatic hydrolysis of wheat straw during bioethanol production, and the spectra were analysed to identify components associated with recalcitrance. A total of 1122 wheat straw samples from nine different locations in Denmark and one location in the United Kingdom, spanning a large variation in genetic material and environmental conditions during growth, were analysed. The FTIR-PAS spectra of non-pretreated wheat straw were correlated with the measured sugar release, determined by a high-throughput pretreatment and enzymatic hydrolysis (HTPH) assay. A partial least square regression (PLSR) calibration model predicting the glucose and xylose release was developed. The interpretation of the regression coefficients revealed a positive correlation between the released glucose and xylose with easily hydrolysable compounds, such as amorphous cellulose and hemicellulose. Additionally, we observed a negative correlation with crystalline cellulose and lignin, which inhibits cellulose and hemicellulose hydrolysis. FTIR-PAS was used as a reliable method for the rapid estimation of sugar release during bioethanol production. The spectra revealed that lignin inhibited the hydrolysis of polysaccharides into monomers, while the crystallinity of cellulose retarded its hydrolysis into glucose. Amorphous cellulose and xylans were found to contribute significantly to the released amounts of glucose and xylose, respectively.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE)

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1259666

Journal Information:: Biotechnology for Biofuels, Journal Name: Biotechnology for Biofuels Journal Issue: 1 Vol. 8; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Enzymatic transformations of cellulose assessed by quantitative high-throughput fourier transform infrared spectroscopy (QHT-FTIR) Corgié, Stéphane C.; Smith, Hanna M.; Walker, Larry P. Biotechnology and Bioengineering, Vol. 108, Issue 7 https://doi.org/10.1002/bit.23098	journal	March 2011
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Prediction of the degradability and ash content of wheat straw from different cultivars using near infrared spectroscopy Bruun, Sander; Jensen, Jacob Wagner; Magid, Jakob Industrial Crops and Products, Vol. 31, Issue 2 https://doi.org/10.1016/j.indcrop.2009.11.011	journal	March 2010
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Cell wall structural changes in wheat straw pretreated for bioethanol production Kristensen, Jan B.; Thygesen, Lisbeth G.; Felby, Claus Biotechnology for Biofuels, Vol. 1, Issue 1 https://doi.org/10.1186/1754-6834-1-5	journal	January 2008
A holistic high-throughput screening framework for biofuel feedstock assessment that characterises variations in soluble sugars and cell wall composition in Sorghum bicolor Martin, Antony P.; Palmer, William M.; Byrt, Caitlin S. Biotechnology for Biofuels, Vol. 6, Issue 1 https://doi.org/10.1186/1754-6834-6-186	journal	January 2013
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Determination of optimal biomass pretreatment strategies for biofuel production: investigation of relationships between surface-exposed polysaccharides and their enzymatic conversion using carbohydrate-binding modules Khatri, Vinay; Meddeb-Mouelhi, Fatma; Adjallé, Kokou Biotechnology for Biofuels, Vol. 11, Issue 1 https://doi.org/10.1186/s13068-018-1145-5	journal	May 2018
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Title: Rapid estimation of sugar release from winter wheat straw during bioethanol production using FTIR-photoacoustic spectroscopy

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