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Title: Middle East Respiratory Syndrome Coronavirus Intra-Host Populations Are Characterized by Numerous High Frequency Variants

Abstract

Middle East respiratory syndrome coronavirus (MERS-CoV) is an emerging human pathogen related to SARS virus. In vitro studies indicate this virus may have a broad host range suggesting an increased pandemic potential. Genetic and epidemiological evidence indicate camels serve as a reservoir for MERS virus but the mechanism of cross species transmission is unclear and many questions remain regarding the susceptibility of humans to infection. Deep sequencing data was obtained from the nasal samples of three camels that had been experimentally infected with a human MERS-CoV isolate. A majority of the genome was covered and average coverage was greater than 12,000x depth. Although only 5 mutations were detected in the consensus sequences, 473 intrahost single nucleotide variants were identified. Lastly, many of these variants were present at high frequencies and could potentially influence viral phenotype and the sensitivity of detection assays that target these regions for primer or probe binding.

Authors:
 [1];  [1];  [1];  [1];  [2];  [3];  [2];  [1]
  1. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
  2. Colorado State Univ., Fort Collins, CO (United States)
  3. National Inst. of Health (NIH), Hamilton, MT (United States)
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE; National Institutes of Health (NIH)
OSTI Identifier:
1259526
Alternate Identifier(s):
OSTI ID: 1375300
Report Number(s):
LLNL-JRNL-676963
Journal ID: ISSN 1932-6203
Grant/Contract Number:  
AC52-07NA27344; S10RR029668; S10RR027303
Resource Type:
Accepted Manuscript
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 11; Journal Issue: 1; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Borucki, Monica K., Lao, Victoria, Hwang, Mona, Gardner, Shea, Adney, Danielle, Munster, Vincent, Bowen, Richard, and Allen, Jonathan E.. Middle East Respiratory Syndrome Coronavirus Intra-Host Populations Are Characterized by Numerous High Frequency Variants. United States: N. p., 2016. Web. doi:10.1371/journal.pone.0146251.
Borucki, Monica K., Lao, Victoria, Hwang, Mona, Gardner, Shea, Adney, Danielle, Munster, Vincent, Bowen, Richard, & Allen, Jonathan E.. Middle East Respiratory Syndrome Coronavirus Intra-Host Populations Are Characterized by Numerous High Frequency Variants. United States. doi:10.1371/journal.pone.0146251.
Borucki, Monica K., Lao, Victoria, Hwang, Mona, Gardner, Shea, Adney, Danielle, Munster, Vincent, Bowen, Richard, and Allen, Jonathan E.. Wed . "Middle East Respiratory Syndrome Coronavirus Intra-Host Populations Are Characterized by Numerous High Frequency Variants". United States. doi:10.1371/journal.pone.0146251. https://www.osti.gov/servlets/purl/1259526.
@article{osti_1259526,
title = {Middle East Respiratory Syndrome Coronavirus Intra-Host Populations Are Characterized by Numerous High Frequency Variants},
author = {Borucki, Monica K. and Lao, Victoria and Hwang, Mona and Gardner, Shea and Adney, Danielle and Munster, Vincent and Bowen, Richard and Allen, Jonathan E.},
abstractNote = {Middle East respiratory syndrome coronavirus (MERS-CoV) is an emerging human pathogen related to SARS virus. In vitro studies indicate this virus may have a broad host range suggesting an increased pandemic potential. Genetic and epidemiological evidence indicate camels serve as a reservoir for MERS virus but the mechanism of cross species transmission is unclear and many questions remain regarding the susceptibility of humans to infection. Deep sequencing data was obtained from the nasal samples of three camels that had been experimentally infected with a human MERS-CoV isolate. A majority of the genome was covered and average coverage was greater than 12,000x depth. Although only 5 mutations were detected in the consensus sequences, 473 intrahost single nucleotide variants were identified. Lastly, many of these variants were present at high frequencies and could potentially influence viral phenotype and the sensitivity of detection assays that target these regions for primer or probe binding.},
doi = {10.1371/journal.pone.0146251},
journal = {PLoS ONE},
number = 1,
volume = 11,
place = {United States},
year = {2016},
month = {1}
}

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