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Title: Degradation of phenolic compounds by the lignocellulose deconstructing thermoacidophilic bacterium Alicyclobacillus Acidocaldarius

Abstract

Alicyclobacillus acidocaldarius, a thermoacidophilic bacterium, has a repertoire of thermo- and acid-stable enzymes that deconstruct lignocellulosic compounds. The work presented here describes the ability of A. acidocaldarius to reduce the concentration of the phenolic compounds: phenol, ferulic acid, ρ-coumaric acid and sinapinic acid during growth conditions. The extent and rate of the removal of these compounds were significantly increased by the presence of micro-molar copper concentrations, suggesting activity by copper oxidases that have been identified in the genome of A. acidocaldarius. Substrate removal kinetics was first order for phenol, ferulic acid, ρ-coumaric acid and sinapinic acid in the presence of 50 μM copper sulfate. In addition, laccase enzyme assays of cellular protein fractions suggested significant activity on a lignin analog between the temperatures of 45 and 90 °C. As a result, this work shows the potential for A. acidocaldarius to degrade phenolic compounds, demonstrating potential relevance to biofuel production and other industrial processes.

Authors:
 [1];  [1];  [2];  [1];  [1];  [1];  [1];  [1]
  1. Idaho National Lab. (INL), Idaho Falls, ID (United States)
  2. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Publication Date:
Research Org.:
Idaho National Lab. (INL), Idaho Falls, ID (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1259505
Report Number(s):
INL/JOU-13-30180
Journal ID: ISSN 1367-5435; PII: 1700
Grant/Contract Number:  
AC07-05ID14517
Resource Type:
Accepted Manuscript
Journal Name:
Journal of Industrial Microbiology and Biotechnology
Additional Journal Information:
Journal Volume: 43; Journal Issue: 1; Journal ID: ISSN 1367-5435
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; Alicyclobacillus acidocaldarius; Thermophiles; Phenolics; Bioremoval

Citation Formats

Aston, John E., Apel, William A., Lee, Brady D., Thompson, David N., Lacey, Jeffrey A., Newby, Deborah T., Reed, David. W., and Thompson, Vicki S. Degradation of phenolic compounds by the lignocellulose deconstructing thermoacidophilic bacterium Alicyclobacillus Acidocaldarius. United States: N. p., 2015. Web. doi:10.1007/s10295-015-1700-z.
Aston, John E., Apel, William A., Lee, Brady D., Thompson, David N., Lacey, Jeffrey A., Newby, Deborah T., Reed, David. W., & Thompson, Vicki S. Degradation of phenolic compounds by the lignocellulose deconstructing thermoacidophilic bacterium Alicyclobacillus Acidocaldarius. United States. doi:10.1007/s10295-015-1700-z.
Aston, John E., Apel, William A., Lee, Brady D., Thompson, David N., Lacey, Jeffrey A., Newby, Deborah T., Reed, David. W., and Thompson, Vicki S. Thu . "Degradation of phenolic compounds by the lignocellulose deconstructing thermoacidophilic bacterium Alicyclobacillus Acidocaldarius". United States. doi:10.1007/s10295-015-1700-z. https://www.osti.gov/servlets/purl/1259505.
@article{osti_1259505,
title = {Degradation of phenolic compounds by the lignocellulose deconstructing thermoacidophilic bacterium Alicyclobacillus Acidocaldarius},
author = {Aston, John E. and Apel, William A. and Lee, Brady D. and Thompson, David N. and Lacey, Jeffrey A. and Newby, Deborah T. and Reed, David. W. and Thompson, Vicki S.},
abstractNote = {Alicyclobacillus acidocaldarius, a thermoacidophilic bacterium, has a repertoire of thermo- and acid-stable enzymes that deconstruct lignocellulosic compounds. The work presented here describes the ability of A. acidocaldarius to reduce the concentration of the phenolic compounds: phenol, ferulic acid, ρ-coumaric acid and sinapinic acid during growth conditions. The extent and rate of the removal of these compounds were significantly increased by the presence of micro-molar copper concentrations, suggesting activity by copper oxidases that have been identified in the genome of A. acidocaldarius. Substrate removal kinetics was first order for phenol, ferulic acid, ρ-coumaric acid and sinapinic acid in the presence of 50 μM copper sulfate. In addition, laccase enzyme assays of cellular protein fractions suggested significant activity on a lignin analog between the temperatures of 45 and 90 °C. As a result, this work shows the potential for A. acidocaldarius to degrade phenolic compounds, demonstrating potential relevance to biofuel production and other industrial processes.},
doi = {10.1007/s10295-015-1700-z},
journal = {Journal of Industrial Microbiology and Biotechnology},
number = 1,
volume = 43,
place = {United States},
year = {2015},
month = {11}
}

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