Degradation of phenolic compounds by the lignocellulose deconstructing thermoacidophilic bacterium Alicyclobacillus Acidocaldarius
Abstract
Alicyclobacillus acidocaldarius, a thermoacidophilic bacterium, has a repertoire of thermo- and acid-stable enzymes that deconstruct lignocellulosic compounds. The work presented here describes the ability of A. acidocaldarius to reduce the concentration of the phenolic compounds: phenol, ferulic acid, ρ-coumaric acid and sinapinic acid during growth conditions. The extent and rate of the removal of these compounds were significantly increased by the presence of micro-molar copper concentrations, suggesting activity by copper oxidases that have been identified in the genome of A. acidocaldarius. Substrate removal kinetics was first order for phenol, ferulic acid, ρ-coumaric acid and sinapinic acid in the presence of 50 μM copper sulfate. In addition, laccase enzyme assays of cellular protein fractions suggested significant activity on a lignin analog between the temperatures of 45 and 90 °C. As a result, this work shows the potential for A. acidocaldarius to degrade phenolic compounds, demonstrating potential relevance to biofuel production and other industrial processes.
- Authors:
-
- grid.417824.c 0000000100207392 Biological and Chemical Processing Department Idaho National Laboratory Idaho Falls ID USA
- grid.451303.0 0000000122183491 Energy and Environment Directorate Pacific Northwest National Laboratory Richland WA USA
- Publication Date:
- Research Org.:
- Idaho National Laboratory (INL), Idaho Falls, ID (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1773514
- Alternate Identifier(s):
- OSTI ID: 1259505
- Report Number(s):
- INL/JOU-13-30180
Journal ID: ISSN 1367-5435
- Grant/Contract Number:
- AC07-05ID14517
- Resource Type:
- Published Article
- Journal Name:
- Journal of Industrial Microbiology and Biotechnology
- Additional Journal Information:
- Journal Name: Journal of Industrial Microbiology and Biotechnology Journal Volume: 43 Journal Issue: 1; Journal ID: ISSN 1367-5435
- Publisher:
- Oxford University Press
- Country of Publication:
- Germany
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; Alicyclobacillus acidocaldarius; Thermophiles; Phenolics; Bioremoval
Citation Formats
Aston, John E., Apel, William A., Lee, Brady D., Thompson, David N., Lacey, Jeffrey A., Newby, Deborah T., Reed, David W., and Thompson, Vicki S. Degradation of phenolic compounds by the lignocellulose deconstructing thermoacidophilic bacterium Alicyclobacillus Acidocaldarius. Germany: N. p., 2016.
Web. doi:10.1007/s10295-015-1700-z.
Aston, John E., Apel, William A., Lee, Brady D., Thompson, David N., Lacey, Jeffrey A., Newby, Deborah T., Reed, David W., & Thompson, Vicki S. Degradation of phenolic compounds by the lignocellulose deconstructing thermoacidophilic bacterium Alicyclobacillus Acidocaldarius. Germany. https://doi.org/10.1007/s10295-015-1700-z
Aston, John E., Apel, William A., Lee, Brady D., Thompson, David N., Lacey, Jeffrey A., Newby, Deborah T., Reed, David W., and Thompson, Vicki S. Fri .
"Degradation of phenolic compounds by the lignocellulose deconstructing thermoacidophilic bacterium Alicyclobacillus Acidocaldarius". Germany. https://doi.org/10.1007/s10295-015-1700-z.
@article{osti_1773514,
title = {Degradation of phenolic compounds by the lignocellulose deconstructing thermoacidophilic bacterium Alicyclobacillus Acidocaldarius},
author = {Aston, John E. and Apel, William A. and Lee, Brady D. and Thompson, David N. and Lacey, Jeffrey A. and Newby, Deborah T. and Reed, David W. and Thompson, Vicki S.},
abstractNote = {Alicyclobacillus acidocaldarius, a thermoacidophilic bacterium, has a repertoire of thermo- and acid-stable enzymes that deconstruct lignocellulosic compounds. The work presented here describes the ability of A. acidocaldarius to reduce the concentration of the phenolic compounds: phenol, ferulic acid, ρ-coumaric acid and sinapinic acid during growth conditions. The extent and rate of the removal of these compounds were significantly increased by the presence of micro-molar copper concentrations, suggesting activity by copper oxidases that have been identified in the genome of A. acidocaldarius. Substrate removal kinetics was first order for phenol, ferulic acid, ρ-coumaric acid and sinapinic acid in the presence of 50 μM copper sulfate. In addition, laccase enzyme assays of cellular protein fractions suggested significant activity on a lignin analog between the temperatures of 45 and 90 °C. As a result, this work shows the potential for A. acidocaldarius to degrade phenolic compounds, demonstrating potential relevance to biofuel production and other industrial processes.},
doi = {10.1007/s10295-015-1700-z},
journal = {Journal of Industrial Microbiology and Biotechnology},
number = 1,
volume = 43,
place = {Germany},
year = {Fri Jan 01 00:00:00 EST 2016},
month = {Fri Jan 01 00:00:00 EST 2016}
}
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Works referencing / citing this record:
Stable-Isotope Probing-Enabled Cultivation of the Indigenous Bacterium Ralstonia sp. Strain M1, Capable of Degrading Phenanthrene and Biphenyl in Industrial Wastewater
journal, May 2019
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