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Title: Thermophilic and thermoacidophilic biopolymer-degrading genes and enzymes from Alicyclobacillus acidocaldarius and related organisms, methods

Patent ·
OSTI ID:1422798

Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods of at least partially degrading, cleaving, or removing polysaccharides, lignocellulose, cellulose, hemicellulose, lignin, starch, chitin, polyhydroxybutyrate, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups using isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius.

Research Organization:
Idaho National Laboratory (INL), Idaho Falls, ID (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC07-991D13727; AC07-051D14517
Assignee:
Battelle Energy Alliance, LLC (Idaho Falls, ID)
Patent Number(s):
9,896,707
Application Number:
15/191,113
OSTI ID:
1422798
Resource Relation:
Patent File Date: 2016 Jun 23
Country of Publication:
United States
Language:
English

References (154)

The first description of an archaeal hemicellulase: the xylanase from Thermococcus zilligii strain AN1 journal November 1999
Genetic elements, proteins, and associated methods including application of additional genetic information to gram (+) thermoacidophiles patent-application November 2011
The nature of lignocellulosics and their pretreatments for enzymatic hydrolysis book January 1982
PCR ligation mutagenesis in transformable streptococci: application and efficiency journal April 2002
Glycosyl hydrolases from hyperthermophiles journal February 1997
Biomass as Feedstock for a Bioenergy and Bioproducts Industry: The Technical Feasibility of a Billion-Ton Annual Supply, April 2005 report April 2005
Synthesis of oligosaccharides, glycolipds, and glycoproteins using bacterial glycosyltransferases patent-application June 2007
Conversion of a β-Ketoacyl Synthase to a Malonyl Decarboxylase by Replacement of the Active-Site Cysteine with Glutamine journal September 1999
Production of a cellulase-free xylanase from agricultural waste materials by a thermotolerant Streptomyces sp. journal October 2001
Lignochemicals book January 1982
Microbial Pretreatment of Biomass: Potential for Reducing Severity of Thermochemical Biomass Pretreatment journal January 2003
Glycosylation Engineering: Controlling personalities tame wild sugars on proteins and natural products journal September 2006
Purification and Properties of Acid Stable Xylanases from Aspergillus kawachii journal January 1992
Protein Engineering Approaches in the Post-Genomic Era journal November 2017
Hackney, F. L., et al. Chondrosarcoma of the jaws journal January 1992
Xylanases in bleaching: From an idea to the industry journal March 1994
Crystal structures of the effector-binding domain of repressor Central glycolytic gene Regulator from Bacillus subtilis reveal ligand-induced structural changes upon binding of several glycolytic intermediates journal August 2008
Expression of microbial proteins in plants for production of plants with improved properties patent-application December 2003
Isolation and characterization of a new family 42 β-galactosidase from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius: Identification of the active site residues journal February 2008
Maltose and Maltodextrin Transport in the Thermoacidophilic Gram-Positive Bacterium Alicyclobacillus acidocaldarius Is Mediated by a High-Affinity Transport System That Includes a Maltose Binding Protein Tolerant to Low pH journal November 2000
journal October 2003
Strand displacement amplification—an isothermal, in vitro DNA amplification technique journal January 1992
Molecular and biotechnological aspects of xylanases journal July 1999
Thermophilic and thermoacidophilic sugar transporter genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods patent June 2011
Polypeptides having haloperoxidase activity patent January 2003
Thermophilic and thermoacidophilic biopolymer-degrading genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods patent June 2012
Comparison of the economics of acid and enzymatic hydrolysis of newsprint journal April 1978
Baculovirus systems for the expression of human gene products journal October 1993
Thermophilic and thermoacidophilic glycosylation genes and enzymes from Alicyclobacillus acidocaldarius and related organisms, methods patent-application October 2009
Characterization of a β-glycosidase from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius journal April 2006
Continuous cultures of fused cells secreting antibody of predefined specificity journal August 1975
Thermophilic and thermoacidophilic metabolism genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods patent December 2015
Lignocellulosic Biomass to Ethanol Process Design and Economics Utilizing Co-Current Dilute Acid Prehydrolysis and Enzymatic Hydrolysis for Corn Stover report June 2002
Genetic disease detection and DNA amplification using cloned thermostable ligase. journal January 1991
Transcriptional control in alicyclobacillus acidocaldarius and associated genes, proteins, and methods patent November 2016
Manufacture of Xylose-Based Fermentation Media from Corncobs by Posthydrolysis of Autohydrolysis Liquors journal January 2001
Rapid chemiluminescent nucleic acid assays for detection of TEM-1 beta-lactamase-mediated penicillin resistance in Neisseria gonorrhoeae and other bacteria. journal January 1988
Thermostable xylanases patent July 2000
A thermoacidophilic endoglucanase (CelB) from Alicyclobacillus acidocaldarius displays high sequence similarity to arabinofuranosidases belonging to family 51 of glycoside hydrolases journal August 2003
Xylanase derived from a Bacillus species, expression vectors for such xylanase and other proteins, host organisms therefor and use thereof patent July 2002
Biochemistry and genetics of microbial xylanases journal June 1996
Xylanase obtained from an anaerobic fungus patent September 1999
Process for pretreating cellulosic substrates and for producing sugar therefrom patent December 1980
Analytical strategies for the use of DNA probes journal February 1988
Probe containing a modified nucleic acid recognizable by specific antibodies and use of this probe to detect and characterize a homologous dna sequence patent March 1992
Preliminary Investigation of Fungal Bioprocessing of Wheat Straw for Production of Straw-Thermoplastic Composites book January 2003
Biotransformation of Toxic Organic and Inorganic Contaminants by Halophilic Bacteria book January 2004
Cellulase-free xylanases from Bacillus and other microorganisms journal February 2000
Thermal and acid tolerant beta-xylosidases, genes encoding, related organisms, and methods patent April 2011
Single molecule detection of RNA reporter probes by amplification with Qβ replicase journal August 1996
Functional reconstitution of a maltose ATP-binding cassette transporter from the thermoacidophilic gram-positive bacterium Alicyclobacillus acidocaldarius journal May 2004
Bacterial glycoproteins: Functions, biosynthesis and applications journal April 2003
Microbial process for producing L-ascorbic acid, D-erythorbic acid, and salts thereof patent August 2004
Overview and Evaluation of Fuel Ethanol from Cellulosic Biomass: Technology, Economics, the Environment, and Policy journal November 1996
Rapid production of thermostable cellulase-free xylanase by a strain of Bacillus subtilis and its properties journal June 2002
Microbial β-Glucosidases: Cloning, Properties, and Applications journal January 2002
Two Glyceraldehyde-3-phosphate Dehydrogenases with Opposite Physiological Roles in a Nonphotosynthetic Bacterium journal May 2000
Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes patent-application April 2011
Thermophilic and thermoacidophilic biopolymer-degrading genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods patent December 2010
Crystallographic and mutational analyses of an extremely acidophilic and acid-stable xylanase: biased distribution of acidic residues and importance of Asp37 for catalysis at low pH journal December 1998
Thermophilic and thermoacidophilic sugar transporter genes and enzymes from Alicyclobacillus acidocaldarius and related organisms, methods patent December 2011
Synthesis of an amplifiable reporter RNA for bioassays journal January 1986
Characterization of a new bacteriophage which infects bacteria of the genus Acidiphilium journal November 1993
Oxygen effects on thermophilic microbial populations in biofilters treating nitric oxide containing off-gas streams journal October 2001
Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes patent July 2013
Thermophilic and Thermoacidophilic Metabolism Genes and Enzymes From Alicyclobacillus Acidocaldarius and Related Organisms, Methods patent-application February 2016
Plasmid, method for construction of the same, microorganism carrying the plasmid and method for cultivation of the microorganism patent November 1986
Hydrolases, nucleic acids encoding them and methods for making and using them patent October 2012
The Synthesis of Bovine Insulin by the Solid Phase Method journal November 1966
Current applications of COS cell based transient expression systems journal October 1993
Cloning and transcriptional analysis of the Thermoanaerobacter ethanolicus strain 39E maltose ABC transport system journal March 2002
Quantitative Saccharification of Wood and Cellulose journal January 1945
Process for hydrolyzing starch without pH adjustment patent-application July 2003
Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system. journal January 1992
Autocatalytic replication of a recombinant RNA journal December 1983
Lignocellulose biodegradation: Fundamentals and applications journal June 2002
Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design journal August 2005
Hyperthermophilic Enzymes: Sources, Uses, and Molecular Mechanisms for Thermostability journal March 2001
Polymerase chain reaction journal November 1989
Transformation of Acidiphilium by electroporation and conjugation journal May 1992
An extremely thermostable xylanase from the thermophilic eubacterium Thermotoga journal July 1991
Alteration and modulation of protein activity by varying post-translational modification patent-application August 2010
Thermoacidophilic Microorganisms and their Novel Biocatalysts journal December 2004
Thermophilic and thermoacidophilic biopolymer-degrading genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods patent-application January 2012
Powers and Pitfalls in Sequence Analysis: The 70% Hurdle journal April 2000
Chemical Makers Try Biotech Paths: Vitamins and a new DuPont polyester are paving the way for biocatalysis use journal June 1998
Recent advances in heterologous gene expression in Escherichia coli journal October 1993
Dilute-Sulfuric Acid Pretreatment of Corn Stover in Pilot-Scale Reactor book January 2003
Thermophilic and thermoacidophilic biopolymer-degrading genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods patent-application August 2009
Thermostable .alpha.-L-arabinofuranosidase from Aureobasidium pullulans patent March 1999
Pretreatment for enhanced hydrolysis of cellulosic biomass journal January 1984
Transcriptional control in alicyclobacillus acidocaldarius and associated genes, proteins, and methods patent-application August 2009
Xylanases, xylanase families and extremophilic xylanases journal January 2005
X-ray Structures of the Maltose–Maltodextrin-binding Protein of the Thermoacidophilic Bacterium Alicyclobacillus acidocaldarius Provide Insight into Acid Stability of Proteins journal January 2004
Measurement of fumonisins in corn with a fiber optic fluoroimmunosensor conference May 1997
Purification and Characterization of a Novel Thermo-Alkali-Stable Catalase from Thermus brockianus journal January 2003
From genes to protein structure and function: novel applications of computational approaches in the genomic era journal January 2000
Specificity of the protein secretory apparatus: secretion of the heat-labile enterotoxin B subunit pentamers by different species of Gram- bacteria journal January 1995
Improved tools for biological sequence comparison. journal April 1988
Tomato plants that exhibit resistance to botrytis cinerea patent-application December 2003
Dilute Acid Pretreatment, Enzymatic Saccharification, and Fermentation of Rice Hulls to Ethanol journal January 2005
Development of gas phase bioreactors for the removal of nitrogen oxides from synthetic flue gas streams journal October 2002
The Path Forward for Biofuels and Biomaterials journal January 2006
Products and processes for in vitro synthesis of biomolecules patent-application September 2006
Partial acid hydrolysis of cellulosic materials as a pretreatment for enzymatic hydrolysis journal July 1980
Microbial Cellulose Utilization: Fundamentals and Biotechnology journal September 2002
Ethanol from lignocellulosic biomass techno-economic performance in short-, middle- and long-term journal April 2005
‘Pseudomonas stutzeri’ strain and process for preparation of xylanase patent December 2004
Microbial Cellulose Utilization: Fundamentals and Biotechnology journal December 2002
Compositions and methods for the synthesis and subsequent modification of uridine-5'-diphosphosulfoquinovose (UDP-SQ) patent-application June 2007
Polypeptides of Alicyclobacillus sp. patent-application April 2007
Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different journal April 2001
Promoters and proteins from Clostridium thermocellum and uses thereof patent-application May 2006
Immunogenicity and protective efficacy of a tuberculosis DNA vaccine journal August 1996
Enzyme and methodology for the treatment of a biomass patent June 2010
Production and purification of a protein fused to a binding protein patent July 1997
Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids journal November 1998
A general method applicable to the search for similarities in the amino acid sequence of two proteins journal March 1970
The Effect of Flow Rate of Compressed Hot Water on Xylan, Lignin, and Total Mass Removal from Corn Stover journal September 2003
Electrotransformation of Acidophilic, Heterotrophic, Gram-negative Bacteria book January 2000
Yeast systems for the expression of heterologous gene products journal October 1993
Generation of transposon insertion mutant libraries for Gram-positive bacteria by electroporation of phage Mu DNA transposition complexes journal April 2005
Regulation of the central glycolytic genes in Bacillus subtilis: binding of the repressor CggR to its single DNA target sequence is modulated by fructose-1,6-bisphosphate journal March 2003
Transcription-based amplification system and detection of amplified human immunodeficiency virus type 1 with a bead-based sandwich hybridization format. journal February 1989
Simultaneous saccharification and fermentation of cellulose: Effect of ethanol on enzymatic saccharification of cellulose journal April 1985
Comparison of pretreatment methods on the basis of available surface area journal January 1992
Lignochemicals journal February 1982
Thermostable xylanase patent June 1999
The acid hydrolysis of sugarcane bagasse hemicellulose to produce xylose, arabinose, glucose and other products journal November 2002
A new class of biodegradable materials: Poly-3-hydroxy-butyrate/steam exploded straw fiber composites. I. Thermal and impact behavior journal September 1993
Biomechanical and Biochemical Pulping of Sugarcane Bagasse with Ceriporiopsis subvermispora Fungal and Xylanase Pretreatments journal March 2001
Molecular diversity of thermophilic cellulolytic and hemicellulolytic bacteria journal February 1999
Potential and utilization of thermophiles and thermostable enzymes in biorefining journal January 2007
Fungal Upgrading of Wheat Straw for Straw-Thermoplastics Production journal January 2004
Interlaboratory testing of methods for assay of xylanase activity journal May 1992
Glycosidases and glycosyl transferases in glycoside and oligosaccharide synthesis journal February 1998
Thermophilic and thermoacidophilic sugar transporter genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods patent-application October 2009
Microbial hemicellulases journal June 2003
Thermal and acid tolerant beta xylosidases, arabinofuranosidases, genes encoding, related organisms, and methods patent April 2013
Thermal and acid tolerant beta xylosidases, arabinofuranosidases, genes encoding, related organisms, and methods patent-application December 2010
Purification, Properties and Structural Aspects of a Thermoacidophilic alpha-Amylase from Alicyclobacillus Acidocaldarius Atcc 27009 journal December 1994
Enzyme and methodology for the treatment of a biomass patent-application May 2007
Gene cloning, sequencing, and characterization of a family 9 endoglucanase (CelA) with an unusual pattern of activity from the thermoacidophile Alicyclobacillus acidocaldarius ATCC27009 journal December 2002
Industrial Scale-Up of pH-Controlled Liquid Hot Water Pretreatment of Corn Fiber for Fuel Ethanol Production journal January 2005
Purification and characterization of an acidophilic xylanase from Aureobasidium pullulans var. melanigenum and sequence analysis of the encoding gene journal January 2001
A comparison of non-radioisotopic hybridization assay methods using fluorescent, chemiluminescent and enzyme labeled synthetic oligodeoxyribonucleotide probes journal January 1988
Identification of essential genes in microorganisms patent-application February 2004
Deciphering the message in protein sequences: tolerance to amino acid substitutions journal March 1990
High temperature and alkaline stable catalase patent-application May 2005
Thermophilic and thermoacidophilic metabolism genes and enzymes from alicyclobacillus acidocaldadarius and related organisms, methods patent-application October 2009
Method of detecting and characterizing a nucleic acid or reactant for the application of this method patent April 1986
Xyloglucan-specific alkaline xyloglucanase from bacillus patent July 2001
Thermophilic Organisms For Conversion Of Lignocellulosic Biomass To Ethanol patent-application September 2009
Thermal and acid tolerant beta-xylosidases, genes encoding, related organisms, and methods patent-application September 2009