A simple novel device for air sampling by electrokinetic capture

Gordon, Julian; Gandhi, Prasanthi; Shekhawat, Gajendra; Frazier, Angel; Hampton-Marcell, Jarrad; Gilbert, Jack A.

doi:10.1186/s40168-015-0141-2

Title: A simple novel device for air sampling by electrokinetic capture

Abstract

A variety of different sampling devices are currently available to acquire air samples for the study of the microbiome of the air. All have a degree of technical complexity that limits deployment. Here, we evaluate the use of a novel device, which has no technical complexity and is easily deployable. An air-cleaning device powered by electrokinetic propulsion has been adapted to provide a universal method for collecting samples of the aerobiome. Plasma-induced charge in aerosol particles causes propulsion to and capture on a counter-electrode. The flow of ions creates net bulk airflow, with no moving parts. A device and electrode assembly have been re-designed from air-cleaning technology to provide an average air flow of 120 lpm. This compares favorably with current air sampling devices based on physical air pumping. Capture efficiency was determined by comparison with a 0.4 μm polycarbonate reference filter, using fluorescent latex particles in a controlled environment chamber. Performance was compared with the same reference filter method in field studies in three different environments. For 23 common fungal species by quantitative polymerase chain reaction (qPCR), there was 100 % sensitivity and apparent specificity of 87%, with the reference filter taken as “gold standard.” Further, bacterial analysis ofmore »« less

Authors:

Gordon, Julian ^[1]; Gandhi, Prasanthi ^[1]; Shekhawat, Gajendra ^[2]; Frazier, Angel ^[3]; Hampton-Marcell, Jarrad ^[3]; Gilbert, Jack A. ^[4]

Inspirotec LLC, Glenview, IL (United States)
Northwestern Univ., Evanston, IL (United States). McCormick School of Engineering and Applied Science
Argonne National Lab. (ANL), Argonne, IL (United States)
Argonne National Lab. (ANL), Argonne, IL (United States); Univ. of Chicago, IL (United States); Univ. of Chicago, IL (United States). Dept. of Surgery

Publication Date:: Sun Dec 27 00:00:00 EST 2015

Research Org.:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1258641

Grant/Contract Number:: AC02-06CH11357

Resource Type:: Accepted Manuscript

Journal Name:: Microbiome

Additional Journal Information:: Journal Volume: 3; Journal Issue: 1; Journal ID: ISSN 2049-2618

Publisher:: BioMed Central

Country of Publication:: United States

Language:: English

Subject:: 47 OTHER INSTRUMENTATION; Atomic force microscopy; Reverse transcriptase PCR; Air sampling; Field study; Aerosol; Nanoparticles; Aerobiome; Amplicon sequencing; Bacteria; Molds

Citation Formats


                    Gordon, Julian, Gandhi, Prasanthi, Shekhawat, Gajendra, Frazier, Angel, Hampton-Marcell, Jarrad, and Gilbert, Jack A. A simple novel device for air sampling by electrokinetic capture.  United States: N. p., 2015. 
Web.  doi:10.1186/s40168-015-0141-2.

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                    Gordon, Julian, Gandhi, Prasanthi, Shekhawat, Gajendra, Frazier, Angel, Hampton-Marcell, Jarrad, & Gilbert, Jack A. A simple novel device for air sampling by electrokinetic capture.  United States.  https://doi.org/10.1186/s40168-015-0141-2

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                    Gordon, Julian, Gandhi, Prasanthi, Shekhawat, Gajendra, Frazier, Angel, Hampton-Marcell, Jarrad, and Gilbert, Jack A. Sun .  
"A simple novel device for air sampling by electrokinetic capture".  United States.  https://doi.org/10.1186/s40168-015-0141-2.  https://www.osti.gov/servlets/purl/1258641.

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@article{osti_1258641,

  title        = {A simple novel device for air sampling by electrokinetic capture},

  author       = {Gordon, Julian and Gandhi, Prasanthi and Shekhawat, Gajendra and Frazier, Angel and Hampton-Marcell, Jarrad and Gilbert, Jack A.},

  abstractNote = {A variety of different sampling devices are currently available to acquire air samples for the study of the microbiome of the air. All have a degree of technical complexity that limits deployment. Here, we evaluate the use of a novel device, which has no technical complexity and is easily deployable. An air-cleaning device powered by electrokinetic propulsion has been adapted to provide a universal method for collecting samples of the aerobiome. Plasma-induced charge in aerosol particles causes propulsion to and capture on a counter-electrode. The flow of ions creates net bulk airflow, with no moving parts. A device and electrode assembly have been re-designed from air-cleaning technology to provide an average air flow of 120 lpm. This compares favorably with current air sampling devices based on physical air pumping. Capture efficiency was determined by comparison with a 0.4 μm polycarbonate reference filter, using fluorescent latex particles in a controlled environment chamber. Performance was compared with the same reference filter method in field studies in three different environments. For 23 common fungal species by quantitative polymerase chain reaction (qPCR), there was 100 % sensitivity and apparent specificity of 87%, with the reference filter taken as “gold standard.” Further, bacterial analysis of 16S RNA by amplicon sequencing showed equivalent community structure captured by the electrokinetic device and the reference filter. Unlike other current air sampling methods, capture of particles is determined by charge and so is not controlled by particle mass. We analyzed particle sizes captured from air, without regard to specific analyte by atomic force microscopy: particles at least as low as 100 nM could be captured from ambient air. This work introduces a very simple plug-and-play device that can sample air at a high-volume flow rate with no moving parts and collect particles down to the sub-micron range. In conclusion, the performance of the device is substantially equivalent to capture by pumping through a filter for microbiome analysis by quantitative PCR and amplicon sequencing.},

  doi          = {10.1186/s40168-015-0141-2},

  journal      = {Microbiome},

  number       = 1,

  volume       = 3,

  place        = {United States},

  year         = {Sun Dec 27 00:00:00 EST 2015},

  month        = {Sun Dec 27 00:00:00 EST 2015}

}

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