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Title: Proton mediated control of biochemical reactions with bioelectronic pH modulation

In Nature, protons (H +) can mediate metabolic process through enzymatic reactions. Examples include glucose oxidation with glucose dehydrogenase to regulate blood glucose level, alcohol dissolution into carboxylic acid through alcohol dehydrogenase, and voltage-regulated H + channels activating bioluminescence in firefly and jellyfish. Artificial devices that control H + currents and H + concentration (pH) are able to actively influence biochemical processes. Here, we demonstrate a biotransducer that monitors and actively regulates pH-responsive enzymatic reactions by monitoring and controlling the flow of H + between PdH x contacts and solution. The present transducer records bistable pH modulation from an “enzymatic flip-flop” circuit that comprises glucose dehydrogenase and alcohol dehydrogenase. Furthermore, the transducer also controls bioluminescence from firefly luciferase by affecting solution pH.
Authors:
 [1] ;  [2] ;  [1] ;  [1] ;  [2]
  1. Univ. of Washington, Seattle, WA (United States)
  2. Univ. of Washington, Seattle, WA (United States); Univ. of California, Santa Cruz, CA (United States)
Publication Date:
Grant/Contract Number:
SC0010441
Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 6; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Research Org:
Univ. of Washington, Seattle, WA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; biomedical engineering; electrical and electronic engineering
OSTI Identifier:
1253913

Deng, Yingxin, Miyake, Takeo, Keene, Scott, Josberger, Erik E., and Rolandi, Marco. Proton mediated control of biochemical reactions with bioelectronic pH modulation. United States: N. p., Web. doi:10.1038/srep24080.
Deng, Yingxin, Miyake, Takeo, Keene, Scott, Josberger, Erik E., & Rolandi, Marco. Proton mediated control of biochemical reactions with bioelectronic pH modulation. United States. doi:10.1038/srep24080.
Deng, Yingxin, Miyake, Takeo, Keene, Scott, Josberger, Erik E., and Rolandi, Marco. 2016. "Proton mediated control of biochemical reactions with bioelectronic pH modulation". United States. doi:10.1038/srep24080. https://www.osti.gov/servlets/purl/1253913.
@article{osti_1253913,
title = {Proton mediated control of biochemical reactions with bioelectronic pH modulation},
author = {Deng, Yingxin and Miyake, Takeo and Keene, Scott and Josberger, Erik E. and Rolandi, Marco},
abstractNote = {In Nature, protons (H+) can mediate metabolic process through enzymatic reactions. Examples include glucose oxidation with glucose dehydrogenase to regulate blood glucose level, alcohol dissolution into carboxylic acid through alcohol dehydrogenase, and voltage-regulated H+ channels activating bioluminescence in firefly and jellyfish. Artificial devices that control H+ currents and H+ concentration (pH) are able to actively influence biochemical processes. Here, we demonstrate a biotransducer that monitors and actively regulates pH-responsive enzymatic reactions by monitoring and controlling the flow of H+ between PdHx contacts and solution. The present transducer records bistable pH modulation from an “enzymatic flip-flop” circuit that comprises glucose dehydrogenase and alcohol dehydrogenase. Furthermore, the transducer also controls bioluminescence from firefly luciferase by affecting solution pH.},
doi = {10.1038/srep24080},
journal = {Scientific Reports},
number = ,
volume = 6,
place = {United States},
year = {2016},
month = {4}
}