Proton mediated control of biochemical reactions with bioelectronic pH modulation

Deng, Yingxin; Miyake, Takeo; Keene, Scott; Josberger, Erik E.; Rolandi, Marco

doi:10.1038/srep24080

Title: Proton mediated control of biochemical reactions with bioelectronic pH modulation

Journal Article · Thu Apr 07 04:00:00 UTC 2016 · Scientific Reports

DOI: https://doi.org/10.1038/srep24080 · OSTI ID:1253913

Deng, Yingxin ^[1]; Miyake, Takeo ^[2]; Keene, Scott ^[3]; Josberger, Erik E. ^[3]; Rolandi, Marco ^[2]

Univ. of Washington, Seattle, WA (United States); University of Washington
Univ. of Washington, Seattle, WA (United States); Univ. of California, Santa Cruz, CA (United States)
Univ. of Washington, Seattle, WA (United States)

In Nature, protons (H⁺) can mediate metabolic process through enzymatic reactions. Examples include glucose oxidation with glucose dehydrogenase to regulate blood glucose level, alcohol dissolution into carboxylic acid through alcohol dehydrogenase, and voltage-regulated H⁺ channels activating bioluminescence in firefly and jellyfish. Artificial devices that control H⁺ currents and H⁺ concentration (pH) are able to actively influence biochemical processes. Here, we demonstrate a biotransducer that monitors and actively regulates pH-responsive enzymatic reactions by monitoring and controlling the flow of H⁺ between PdH_x contacts and solution. The present transducer records bistable pH modulation from an “enzymatic flip-flop” circuit that comprises glucose dehydrogenase and alcohol dehydrogenase. Furthermore, the transducer also controls bioluminescence from firefly luciferase by affecting solution pH.

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Research Organization:: Univ. of Washington, Seattle, WA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: SC0010441

OSTI ID:: 1253913

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Vol. 6; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Endogenous Bioelectrics in Development, Cancer, and Regeneration: Drugs and Bioelectronic Devices as Electroceuticals for Regenerative Medicine Levin, Michael; Selberg, John; Rolandi, Marco iScience, Vol. 22 https://doi.org/10.1016/j.isci.2019.11.023	journal	December 2019
A protonic biotransducer controlling mitochondrial ATP synthesis Zhang, Z.; Kashiwagi, H.; Kimura, S. Scientific Reports, Vol. 8, Issue 1 https://doi.org/10.1038/s41598-018-28435-5	journal	July 2018
A non-enzymatic glucose sensor enabled by bioelectronic pH control Strakosas, Xenofon; Selberg, John; Pansodtee, Pattawong Scientific Reports, Vol. 9, Issue 1 https://doi.org/10.1038/s41598-019-46302-9	journal	July 2019
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Taking Electrons out of Bioelectronics: From Bioprotonic Transistors to Ion Channels Strakosas, Xenofon; Selberg, John; Hemmatian, Zahra Advanced Science, Vol. 4, Issue 7 https://doi.org/10.1002/advs.201600527	journal	March 2017
A Bioelectronic Platform Modulates pH in Biologically Relevant Conditions Strakosas, Xenofon; Selberg, John; Zhang, Xiaolin Advanced Science, Vol. 6, Issue 7 https://doi.org/10.1002/advs.201800935	journal	January 2019
A protonic biotransducer controlling mitochondrial ATP synthesis Zhang, Z.; Kashiwagi, H.; Kimura, S. Scientific Reports, Vol. 8, Issue 1 https://doi.org/10.1038/s41598-018-28435-5	journal	July 2018
A non-enzymatic glucose sensor enabled by bioelectronic pH control Strakosas, Xenofon; Selberg, John; Pansodtee, Pattawong Scientific Reports, Vol. 9, Issue 1 https://doi.org/10.1038/s41598-019-46302-9	journal	July 2019
Bio-inspired protonic memristor devices based on metal complexes with proton-coupled electron transfer Hiruma, Yusuke; Yoshikawa, Kai; Haga, Masa-aki Faraday Discussions, Vol. 213 https://doi.org/10.1039/c8fd00098k	journal	January 2019
Enhanced protonic conductivity and IFET behavior in individual proton-doped electrospun chitosan fibers Lee, Woo-Kyung; Pietron, Jeremy J.; Kidwell, David A. Journal of Materials Chemistry C, Vol. 7, Issue 35 https://doi.org/10.1039/c9tc02452b	journal	January 2019
Proton Conductivity of Glycosaminoglycans Selberg, John; Jia, Manping; Rolandi, Marco PLOS ONE https://doi.org/10.1101/388686	journal	August 2018
Proton conductivity of glycosaminoglycans Selberg, John; Jia, Manping; Rolandi, Marco PLOS ONE, Vol. 14, Issue 3 https://doi.org/10.1371/journal.pone.0202713	journal	March 2019

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