Comparative proteomic analysis of Desulfotomaculum reducens MI-1: Insights into the metabolic versatility of a gram-positive sulfate- and metal-reducing bacterium
Abstract
In this study, the proteomes of the metabolically versatile and poorly characterized Gram-positive bacterium Desulfotomaculum reducens MI-1 were compared across four cultivation conditions including sulfate reduction, soluble Fe(III) reduction, insoluble Fe(III) reduction, and pyruvate fermentation. Collectively across conditions, we observed at high confidence ~38% of genome-encoded proteins. Here, we focus on proteins that display significant differential abundance on conditions tested. To the best of our knowledge, this is the first full-proteome study focused on a Gram-positive organism cultivated either on sulfate or metal-reducing conditions. Several proteins with uncharacterized function encoded within heterodisulfide reductase (hdr)-containing loci were upregulated on either sulfate (Dred_0633-4, Dred_0689-90, and Dred_1325-30) or Fe(III)-citrate-reducing conditions (Dred_0432-3 and Dred_1778-84). Two of these hdr-containing loci display homology to recently described flavin-based electron bifurcation (FBEB) pathways (Dred_1325-30 and Dred_1778-84). Additionally, we propose that a cluster of proteins, which is homologous to a described FBEB lactate dehydrogenase (LDH) complex, is performing lactate oxidation in D. reducens (Dred_0367-9). Analysis of the putative sulfate reduction machinery in D. reducens revealed that most of these proteins are constitutively expressed across cultivation conditions tested. In addition, peptides from the single multiheme c-type cytochrome (MHC) in the genome were exclusively observed on the insoluble Fe(III) condition, suggestingmore »
- Authors:
-
- Cornell Univ., Ithaca, NY (United States)
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Publication Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); Cornell Univ., Ithaca, NY (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1249361
- Alternate Identifier(s):
- OSTI ID: 1239630
- Report Number(s):
- PNNL-SA-115268
Journal ID: ISSN 1664-302X
- Grant/Contract Number:
- SC0006644; AC05-76RL01830
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Frontiers in Microbiology
- Additional Journal Information:
- Journal Volume: 7; Journal ID: ISSN 1664-302X
- Publisher:
- Frontiers Research Foundation
- Country of Publication:
- United States
- Language:
- English
- Subject:
- Fe(III) reduction; sulfate reduction; Desulfotomaculum reducens; gram-positive bacteria; comparative proteomic analysis; flavin-based electron bifurcation; heterodisulfide reductase; 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Otwell, Anne E., Callister, Stephen J., Zink, Erika M., Smith, Richard D., and Richardson, Ruth E. Comparative proteomic analysis of Desulfotomaculum reducens MI-1: Insights into the metabolic versatility of a gram-positive sulfate- and metal-reducing bacterium. United States: N. p., 2016.
Web. doi:10.3389/fmicb.2016.00191.
Otwell, Anne E., Callister, Stephen J., Zink, Erika M., Smith, Richard D., & Richardson, Ruth E. Comparative proteomic analysis of Desulfotomaculum reducens MI-1: Insights into the metabolic versatility of a gram-positive sulfate- and metal-reducing bacterium. United States. https://doi.org/10.3389/fmicb.2016.00191
Otwell, Anne E., Callister, Stephen J., Zink, Erika M., Smith, Richard D., and Richardson, Ruth E. Fri .
"Comparative proteomic analysis of Desulfotomaculum reducens MI-1: Insights into the metabolic versatility of a gram-positive sulfate- and metal-reducing bacterium". United States. https://doi.org/10.3389/fmicb.2016.00191. https://www.osti.gov/servlets/purl/1249361.
@article{osti_1249361,
title = {Comparative proteomic analysis of Desulfotomaculum reducens MI-1: Insights into the metabolic versatility of a gram-positive sulfate- and metal-reducing bacterium},
author = {Otwell, Anne E. and Callister, Stephen J. and Zink, Erika M. and Smith, Richard D. and Richardson, Ruth E.},
abstractNote = {In this study, the proteomes of the metabolically versatile and poorly characterized Gram-positive bacterium Desulfotomaculum reducens MI-1 were compared across four cultivation conditions including sulfate reduction, soluble Fe(III) reduction, insoluble Fe(III) reduction, and pyruvate fermentation. Collectively across conditions, we observed at high confidence ~38% of genome-encoded proteins. Here, we focus on proteins that display significant differential abundance on conditions tested. To the best of our knowledge, this is the first full-proteome study focused on a Gram-positive organism cultivated either on sulfate or metal-reducing conditions. Several proteins with uncharacterized function encoded within heterodisulfide reductase (hdr)-containing loci were upregulated on either sulfate (Dred_0633-4, Dred_0689-90, and Dred_1325-30) or Fe(III)-citrate-reducing conditions (Dred_0432-3 and Dred_1778-84). Two of these hdr-containing loci display homology to recently described flavin-based electron bifurcation (FBEB) pathways (Dred_1325-30 and Dred_1778-84). Additionally, we propose that a cluster of proteins, which is homologous to a described FBEB lactate dehydrogenase (LDH) complex, is performing lactate oxidation in D. reducens (Dred_0367-9). Analysis of the putative sulfate reduction machinery in D. reducens revealed that most of these proteins are constitutively expressed across cultivation conditions tested. In addition, peptides from the single multiheme c-type cytochrome (MHC) in the genome were exclusively observed on the insoluble Fe(III) condition, suggesting that this MHC may play a role in reduction of insoluble metals.},
doi = {10.3389/fmicb.2016.00191},
journal = {Frontiers in Microbiology},
number = ,
volume = 7,
place = {United States},
year = {Fri Feb 19 00:00:00 EST 2016},
month = {Fri Feb 19 00:00:00 EST 2016}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 9 works
Citation information provided by
Web of Science
Web of Science
Figures / Tables:
TABLE 1: Global overview of D. reducens comparative proteomic analysis.
All figures and tables
(10 total)
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
MicrobesOnline: an integrated portal for comparative and functional genomics
journal, November 2009
- Dehal, P. S.; Joachimiak, M. P.; Price, M. N.
- Nucleic Acids Research, Vol. 38, Issue suppl_1, p. D396-D400
The biogeochemistry and bioremediation of uranium and other priority radionuclides
journal, January 2014
- Newsome, Laura; Morris, Katherine; Lloyd, Jonathan R.
- Chemical Geology, Vol. 363
DAnTE: a statistical tool for quantitative analysis of -omics data
journal, May 2008
- Polpitiya, A. D.; Qian, W. -J.; Jaitly, N.
- Bioinformatics, Vol. 24, Issue 13
Genome analysis of Desulfotomaculum gibsoniae strain GrollT a highly versatile Gram-positive sulfate-reducing bacterium
journal, March 2014
- Kuever, Jan; Visser, Michael; Loeffler, Claudia
- Standards in Genomic Sciences, Vol. 9, Issue 3
A trans-outer membrane porin-cytochrome protein complex for extracellular electron transfer by G eobacter sulfurreducens PCA : A trans-outer membrane electron transfer complex
journal, September 2014
- Liu, Yimo; Wang, Zheming; Liu, Juan
- Environmental Microbiology Reports, Vol. 6, Issue 6
Characterization of the surfaceome of the metal-reducing bacterium Desulfotomaculum reducens
journal, August 2014
- Dalla Vecchia, Elena; Shao, Paul P.; Suvorova, Elena
- Frontiers in Microbiology, Vol. 5
A protein trisulfide couples dissimilatory sulfate reduction to energy conservation
journal, December 2015
- Santos, A. A.; Venceslau, S. S.; Grein, F.
- Science, Vol. 350, Issue 6267
A modification of the methylene blue method to measure bacterial sulfide production in feces
journal, April 1992
- Strocchi, Alessandra; Furne, Julie K.; Levitt, Michael D.
- Journal of Microbiological Methods, Vol. 15, Issue 2
The Response of Desulfotomaculum reducens MI-1 to U(VI) Exposure: A Transcriptomic Study
journal, June 2011
- Junier, Pilar; Vecchia, Elena Dalla; Bernier-Latmani, Rizlan
- Geomicrobiology Journal, Vol. 28, Issue 5-6
Identification of proteins capable of metal reduction from the proteome of the Gram-positive bacterium D esulfotomaculum reducens MI-1 using an NADH-based activity assay : Metal reductases from Gram-positive metal reducer
journal, January 2015
- Otwell, Anne Elyse; Sherwood, Robert W.; Zhang, Sheng
- Environmental Microbiology, Vol. 17, Issue 6
The “bacterial heterodisulfide” DsrC is a key protein in dissimilatory sulfur metabolism
journal, July 2014
- Venceslau, S. S.; Stockdreher, Y.; Dahl, C.
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1837, Issue 7
A systematic investigation of multiheme c-type cytochromes in prokaryotes
journal, January 2010
- Sharma, Shailesh; Cavallaro, Gabriele; Rosato, Antonio
- JBIC Journal of Biological Inorganic Chemistry, Vol. 15, Issue 4
Proteome of Geobacter sulfurreducens grown with Fe(III) oxide or Fe(III) citrate as the electron acceptor
journal, December 2008
- Ding, Yan-Huai R.; Hixson, Kim K.; Aklujkar, Muktak A.
- Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1784, Issue 12
Global analysis of the Deinococcus radiodurans proteome by using accurate mass tags
journal, August 2002
- Lipton, M. S.; Pasa-Tolic, L.; Anderson, G. A.
- Proceedings of the National Academy of Sciences, Vol. 99, Issue 17
Coupled Ferredoxin and Crotonyl Coenzyme A (CoA) Reduction with NADH Catalyzed by the Butyryl-CoA Dehydrogenase/Etf Complex from Clostridium kluyveri
journal, November 2007
- Li, F.; Hinderberger, J.; Seedorf, H.
- Journal of Bacteriology, Vol. 190, Issue 3
IMG 4 version of the integrated microbial genomes comparative analysis system
journal, October 2013
- Markowitz, Victor M.; Chen, I-Min A.; Palaniappan, Krishna
- Nucleic Acids Research, Vol. 42, Issue D1
Changes in Protein Expression Across Laboratory and Field Experiments in Geobacter bemidjiensis
journal, February 2015
- Merkley, Eric D.; Wrighton, Kelly C.; Castelle, Cindy J.
- Journal of Proteome Research, Vol. 14, Issue 3
Measurement of protein using bicinchoninic acid
journal, October 1985
- Smith, P. K.; Krohn, R. I.; Hermanson, G. T.
- Analytical Biochemistry, Vol. 150, Issue 1
Genome analyses of the carboxydotrophic sulfate-reducers Desulfotomaculum nigrificans and Desulfotomaculum carboxydivorans and reclassification of Desulfotomaculum caboxydivorans as a later synonym of Desulfotomaculum nigrificans
journal, March 2014
- Visser, Michael; Parshina, Sofiya N.; Alves, Joana I.
- Standards in Genomic Sciences, Vol. 9, Issue 3
Uranium Reduction
journal, October 2006
- Wall, Judy D.; Krumholz, Lee R.
- Annual Review of Microbiology, Vol. 60, Issue 1
Microbial Reduction of Fe(III) under Alkaline Conditions Relevant to Geological Disposal
journal, March 2013
- Williamson, Adam J.; Morris, Katherine; Shaw, Sam
- Applied and Environmental Microbiology, Vol. 79, Issue 11
A Comparative Genomic Analysis of Energy Metabolism in Sulfate Reducing Bacteria and Archaea
journal, January 2011
- Pereira, Inês A. Cardoso; Ramos, Ana Raquel; Grein, Fabian
- Frontiers in Microbiology, Vol. 2
The FlxABCD-HdrABC proteins correspond to a novel NADH dehydrogenase/heterodisulfide reductase widespread in anaerobic bacteria and involved in ethanol metabolism in D esulfovibrio vulgaris Hildenborough : Role of FlxABCD-HdrABC in
journal, January 2015
- Ramos, Ana Raquel; Grein, Fabian; Oliveira, Gonçalo P.
- Environmental Microbiology, Vol. 17, Issue 7
Molecular Underpinnings of Fe(III) Oxide Reduction by Shewanella Oneidensis MR-1
journal, January 2012
- Shi, Liang; Rosso, Kevin M.; Clarke, Tomas A.
- Frontiers in Microbiology, Vol. 3
Characterizing Microbial Community and Geochemical Dynamics at Hydrothermal Vents Using Osmotically Driven Continuous Fluid Samplers
journal, April 2013
- Robidart, Julie; Callister, Stephen J.; Song, Pengfei
- Environmental Science & Technology, Vol. 47, Issue 9
Energy Conservation via Electron-Transferring Flavoprotein in Anaerobic Bacteria
journal, November 2007
- Herrmann, G.; Jayamani, E.; Mai, G.
- Journal of Bacteriology, Vol. 190, Issue 3
A Statistical Method for Assessing Peptide Identification Confidence in Accurate Mass and Time Tag Proteomics
journal, August 2011
- Stanley, Jeffrey R.; Adkins, Joshua N.; Slysz, Gordon W.
- Analytical Chemistry, Vol. 83, Issue 16
Fe(III) reduction during pyruvate fermentation by Desulfotomaculum reducens strain MI-1
journal, November 2013
- Dalla Vecchia, E.; Suvorova, E. I.; Maillard, J.
- Geobiology, Vol. 12, Issue 1
The genome of the Gram-positive metal- and sulfate-reducing bacterium Desulfotomaculum reducens strain MI-1: The genome of Desulfotomaculum reducens
journal, June 2010
- Junier, Pilar; Junier, Thomas; Podell, Sheila
- Environmental Microbiology
Desulfotomaculum spp. and related gram-positive sulfate-reducing bacteria in deep subsurface environments
journal, January 2013
- Aüllo, Thomas; Ranchou-Peyruse, Anthony; Ollivier, Bernard
- Frontiers in Microbiology, Vol. 4
Desulfotomaculum hydrothermale sp. nov., a thermophilic sulfate-reducing bacterium isolated from a terrestrial Tunisian hot spring
journal, November 2008
- Haouari, O.; Fardeau, M. -L.; Cayol, J. -L.
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 58, Issue 11
Universal sample preparation method for proteome analysis
journal, April 2009
- Wiśniewski, Jacek R.; Zougman, Alexandre; Nagaraj, Nagarjuna
- Nature Methods, Vol. 6, Issue 5
Isolation of microorganisms involved in reduction of crystalline iron(III) oxides in natural environments
journal, May 2015
- Hori, Tomoyuki; Aoyagi, Tomo; Itoh, Hideomi
- Frontiers in Microbiology, Vol. 6
Energy conservation via electron bifurcating ferredoxin reduction and proton/Na+ translocating ferredoxin oxidation
journal, February 2013
- Buckel, Wolfgang; Thauer, Rudolf K.
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1827, Issue 2
A novel mode of lactate metabolism in strictly anaerobic bacteria: A novel mode of lactate metabolism in anaerobes
journal, May 2014
- Weghoff, Marie Charlotte; Bertsch, Johannes; Müller, Volker
- Environmental Microbiology, Vol. 17, Issue 3
Significant Association between Sulfate-Reducing Bacteria and Uranium-Reducing Microbial Communities as Revealed by a Combined Massively Parallel Sequencing-Indicator Species Approach
journal, August 2010
- Cardenas, E.; Wu, W. -M.; Leigh, M. B.
- Applied and Environmental Microbiology, Vol. 76, Issue 20
The ‘porin-cytochrome’ model for microbe-to-mineral electron transfer: Microbe-to-mineral electron transfer
journal, May 2012
- Richardson, David J.; Butt, Julea N.; Fredrickson, Jim K.
- Molecular Microbiology, Vol. 85, Issue 2
The roles of outer membrane cytochromes of Shewanella and Geobacter in extracellular electron transfer
journal, August 2009
- Shi, Liang; Richardson, David J.; Wang, Zheming
- Environmental Microbiology Reports, Vol. 1, Issue 4
Microbial Populations Stimulated for Hexavalent Uranium Reduction in Uranium Mine Sediment
journal, March 2003
- Suzuki, Y.; Kelly, S. D.; Kemner, K. M.
- Applied and Environmental Microbiology, Vol. 69, Issue 3
Indirect and suboptimal control of gene expression is widespread in bacteria
journal, January 2013
- Price, Morgan N.; Deutschbauer, Adam M.; Skerker, Jeffrey M.
- Molecular Systems Biology, Vol. 9, Issue 1
Unifying concepts in anaerobic respiration: Insights from dissimilatory sulfur metabolism
journal, February 2013
- Grein, Fabian; Ramos, Ana Raquel; Venceslau, Sofia S.
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1827, Issue 2
Sulfate-reducing bacterium grows with Cr(VI), U(VI), Mn(IV), and Fe(III) as electron acceptors
journal, May 1998
- Tebo, Bradley M.; Obraztsova, Anna Ya
- FEMS Microbiology Letters, Vol. 162, Issue 1
Bioenergetic challenges of microbial iron metabolisms
journal, July 2011
- Bird, Lina J.; Bonnefoy, Violaine; Newman, Dianne K.
- Trends in Microbiology, Vol. 19, Issue 7
Comparison of aerobic and photosynthetic Rhodobacter sphaeroides 2.4.1 proteomes
journal, December 2006
- Callister, Stephen J.; Nicora, Carrie D.; Zeng, Xiaohua
- Journal of Microbiological Methods, Vol. 67, Issue 3
Spectral Probabilities and Generating Functions of Tandem Mass Spectra: A Strike against Decoy Databases
journal, August 2008
- Kim, Sangtae; Gupta, Nitin; Pevzner, Pavel A.
- Journal of Proteome Research, Vol. 7, Issue 8
PSORTb 3.0: improved protein subcellular localization prediction with refined localization subcategories and predictive capabilities for all prokaryotes
journal, May 2010
- Yu, Nancy Y.; Wagner, James R.; Laird, Matthew R.
- Bioinformatics, Vol. 26, Issue 13
Comparative Bacterial Proteomics: Analysis of the Core Genome Concept
journal, February 2008
- Callister, Stephen J.; McCue, Lee Ann; Turse, Joshua E.
- PLoS ONE, Vol. 3, Issue 2
Reversed-phase chromatography with multiple fraction concatenation strategy for proteome profiling of human MCF10A cells
journal, April 2011
- Wang, Yuexi; Yang, Feng; Gritsenko, Marina A.
- PROTEOMICS, Vol. 11, Issue 10
Reversed-phase chromatography with multiple fraction concatenation strategy for proteome profiling of human MCF10A cells
journal, April 2011
- Wang, Yuexi; Yang, Feng; Gritsenko, Marina A.
- PROTEOMICS, Vol. 11, Issue 10
Evaluating the effects of causes of death on postmortem interval estimation by ATR-FTIR spectroscopy
journal, March 2019
- Zhang, Kai; Wang, Qi; Liu, Ruina
- International Journal of Legal Medicine, Vol. 134, Issue 2
A systematic investigation of multiheme c-type cytochromes in prokaryotes
journal, January 2010
- Sharma, Shailesh; Cavallaro, Gabriele; Rosato, Antonio
- JBIC Journal of Biological Inorganic Chemistry, Vol. 15, Issue 4
Energy conservation via electron bifurcating ferredoxin reduction and proton/Na+ translocating ferredoxin oxidation
journal, February 2013
- Buckel, Wolfgang; Thauer, Rudolf K.
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1827, Issue 2
Unifying concepts in anaerobic respiration: Insights from dissimilatory sulfur metabolism
journal, February 2013
- Grein, Fabian; Ramos, Ana Raquel; Venceslau, Sofia S.
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1827, Issue 2
The “bacterial heterodisulfide” DsrC is a key protein in dissimilatory sulfur metabolism
journal, July 2014
- Venceslau, S. S.; Stockdreher, Y.; Dahl, C.
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1837, Issue 7
Proteome of Geobacter sulfurreducens grown with Fe(III) oxide or Fe(III) citrate as the electron acceptor
journal, December 2008
- Ding, Yan-Huai R.; Hixson, Kim K.; Aklujkar, Muktak A.
- Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1784, Issue 12
The biogeochemistry and bioremediation of uranium and other priority radionuclides
journal, January 2014
- Newsome, Laura; Morris, Katherine; Lloyd, Jonathan R.
- Chemical Geology, Vol. 363
Comparison of aerobic and photosynthetic Rhodobacter sphaeroides 2.4.1 proteomes
journal, December 2006
- Callister, Stephen J.; Nicora, Carrie D.; Zeng, Xiaohua
- Journal of Microbiological Methods, Vol. 67, Issue 3
Bioenergetic challenges of microbial iron metabolisms
journal, July 2011
- Bird, Lina J.; Bonnefoy, Violaine; Newman, Dianne K.
- Trends in Microbiology, Vol. 19, Issue 7
A Statistical Method for Assessing Peptide Identification Confidence in Accurate Mass and Time Tag Proteomics
journal, August 2011
- Stanley, Jeffrey R.; Adkins, Joshua N.; Slysz, Gordon W.
- Analytical Chemistry, Vol. 83, Issue 16
Changes in Protein Expression Across Laboratory and Field Experiments in Geobacter bemidjiensis
journal, February 2015
- Merkley, Eric D.; Wrighton, Kelly C.; Castelle, Cindy J.
- Journal of Proteome Research, Vol. 14, Issue 3
Spectral Probabilities and Generating Functions of Tandem Mass Spectra: A Strike against Decoy Databases
journal, August 2008
- Kim, Sangtae; Gupta, Nitin; Pevzner, Pavel A.
- Journal of Proteome Research, Vol. 7, Issue 8
Indirect and suboptimal control of gene expression is widespread in bacteria
journal, January 2013
- Price, Morgan N.; Deutschbauer, Adam M.; Skerker, Jeffrey M.
- Molecular Systems Biology, Vol. 9, Issue 1
DAnTE: a statistical tool for quantitative analysis of -omics data
journal, May 2008
- Polpitiya, A. D.; Qian, W. -J.; Jaitly, N.
- Bioinformatics, Vol. 24, Issue 13
PSORTb 3.0: improved protein subcellular localization prediction with refined localization subcategories and predictive capabilities for all prokaryotes
journal, May 2010
- Yu, Nancy Y.; Wagner, James R.; Laird, Matthew R.
- Bioinformatics, Vol. 26, Issue 13
MicrobesOnline: an integrated portal for comparative and functional genomics
journal, November 2009
- Dehal, P. S.; Joachimiak, M. P.; Price, M. N.
- Nucleic Acids Research, Vol. 38, Issue suppl_1, p. D396-D400
IMG 4 version of the integrated microbial genomes comparative analysis system
journal, October 2013
- Markowitz, Victor M.; Chen, I-Min A.; Palaniappan, Krishna
- Nucleic Acids Research, Vol. 42, Issue D1
Desulfotomaculum hydrothermale sp. nov., a thermophilic sulfate-reducing bacterium isolated from a terrestrial Tunisian hot spring
journal, November 2008
- Haouari, O.; Fardeau, M. -L.; Cayol, J. -L.
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 58, Issue 11
Identification of proteins capable of metal reduction from the proteome of the Gram-positive bacterium D esulfotomaculum reducens MI-1 using an NADH-based activity assay : Metal reductases from Gram-positive metal reducer
journal, January 2015
- Otwell, Anne Elyse; Sherwood, Robert W.; Zhang, Sheng
- Environmental Microbiology, Vol. 17, Issue 6
The FlxABCD-HdrABC proteins correspond to a novel NADH dehydrogenase/heterodisulfide reductase widespread in anaerobic bacteria and involved in ethanol metabolism in D esulfovibrio vulgaris Hildenborough : Role of FlxABCD-HdrABC in
journal, January 2015
- Ramos, Ana Raquel; Grein, Fabian; Oliveira, Gonçalo P.
- Environmental Microbiology, Vol. 17, Issue 7
A trans-outer membrane porin-cytochrome protein complex for extracellular electron transfer by G eobacter sulfurreducens PCA : A trans-outer membrane electron transfer complex
journal, September 2014
- Liu, Yimo; Wang, Zheming; Liu, Juan
- Environmental Microbiology Reports, Vol. 6, Issue 6
Fe(III) reduction during pyruvate fermentation by Desulfotomaculum reducens strain MI-1
journal, November 2013
- Dalla Vecchia, E.; Suvorova, E. I.; Maillard, J.
- Geobiology, Vol. 12, Issue 1
Sulfate-reducing bacterium grows with Cr(VI), U(VI), Mn(IV), and Fe(III) as electron acceptors
journal, May 1998
- Tebo, Bradley M.; Obraztsova, Anna Ya
- FEMS Microbiology Letters, Vol. 162, Issue 1
The roles of outer membrane cytochromes of Shewanella and Geobacter in extracellular electron transfer
journal, August 2009
- Shi, Liang; Richardson, David J.; Wang, Zheming
- Environmental Microbiology Reports, Vol. 1, Issue 4
A protein trisulfide couples dissimilatory sulfate reduction to energy conservation
journal, December 2015
- Santos, A. A.; Venceslau, S. S.; Grein, F.
- Science, Vol. 350, Issue 6267
Proteomic Analysis of Stationary Phase in the Marine Bacterium "Candidatus Pelagibacter ubique"
journal, May 2008
- Sowell, S. M.; Norbeck, A. D.; Lipton, M. S.
- Applied and Environmental Microbiology, Vol. 74, Issue 13
Rapid Assay for Microbially Reducible Ferric Iron in Aquatic Sediments
journal, January 1987
- Lovley, Derek R.; Phillips, Elizabeth J. P.
- Applied and Environmental Microbiology, Vol. 53, Issue 7
Enumeration and Characterization of Iron(III)-Reducing Microbial Communities from Acidic Subsurface Sediments Contaminated with Uranium(VI)
journal, December 2003
- Petrie, L.; North, N. N.; Dollhopf, S. L.
- Applied and Environmental Microbiology, Vol. 69, Issue 12, p. 7467-7479
Classic Spotlight: Electron Bifurcation, a Unifying Concept for Energy Conservation in Anaerobes
journal, April 2016
- Metcalf, William W.
- Journal of Bacteriology, Vol. 198, Issue 9
Multiple Lateral Transfers of Dissimilatory Sulfite Reductase Genes between Major Lineages of Sulfate-Reducing Prokaryotes
journal, October 2001
- Klein, M.; Friedrich, M.; Roger, A. J.
- Journal of Bacteriology, Vol. 183, Issue 20
Lateral Gene Transfer of Dissimilatory (Bi)Sulfite Reductase Revisited
journal, March 2005
- Zverlov, V.; Klein, M.; Lucker, S.
- Journal of Bacteriology, Vol. 187, Issue 6
Uranium Reduction
journal, October 2006
- Wall, Judy D.; Krumholz, Lee R.
- Annual Review of Microbiology, Vol. 60, Issue 1
Comparative Bacterial Proteomics: Analysis of the Core Genome Concept
journal, February 2008
- Callister, Stephen J.; McCue, Lee Ann; Turse, Joshua E.
- PLoS ONE, Vol. 3, Issue 2
A Comparative Genomic Analysis of Energy Metabolism in Sulfate Reducing Bacteria and Archaea
journal, January 2011
- Pereira, Inês A. Cardoso; Ramos, Ana Raquel; Grein, Fabian
- Frontiers in Microbiology, Vol. 2
Molecular Underpinnings of Fe(III) Oxide Reduction by Shewanella Oneidensis MR-1
journal, January 2012
- Shi, Liang; Rosso, Kevin M.; Clarke, Tomas A.
- Frontiers in Microbiology, Vol. 3
Desulfotomaculum spp. and related gram-positive sulfate-reducing bacteria in deep subsurface environments
journal, January 2013
- Aüllo, Thomas; Ranchou-Peyruse, Anthony; Ollivier, Bernard
- Frontiers in Microbiology, Vol. 4
Characterization of the surfaceome of the metal-reducing bacterium Desulfotomaculum reducens
journal, August 2014
- Dalla Vecchia, Elena; Shao, Paul P.; Suvorova, Elena
- Frontiers in Microbiology, Vol. 5
Genome analyses of the carboxydotrophic sulfate-reducers Desulfotomaculum nigrificans and Desulfotomaculum carboxydivorans and reclassification of Desulfotomaculum caboxydivorans as a later synonym of Desulfotomaculum nigrificans
journal, March 2014
- Visser, Michael; Parshina, Sofiya N.; Alves, Joana I.
- Standards in Genomic Sciences, Vol. 9, Issue 3
Genome analysis of Desulfotomaculum gibsoniae strain GrollT a highly versatile Gram-positive sulfate-reducing bacterium
journal, March 2014
- Kuever, Jan; Visser, Michael; Loeffler, Claudia
- Standards in Genomic Sciences, Vol. 9, Issue 3
Works referencing / citing this record:
Ecogenomics reveals community interactions in a long-term methanogenic bioreactor and a rapid switch to sulfate-reducing conditions
journal, March 2020
- St. James, Andrew R.; Richardson, Ruth E.
- FEMS Microbiology Ecology, Vol. 96, Issue 5
Flavin-Based Electron Bifurcation, Ferredoxin, Flavodoxin, and Anaerobic Respiration With Protons (Ech) or NAD+ (Rnf) as Electron Acceptors: A Historical Review
journal, March 2018
- Buckel, Wolfgang; Thauer, Rudolf K.
- Frontiers in Microbiology, Vol. 9
Insights into the Evolution of Host Association through the Isolation and Characterization of a Novel Human Periodontal Pathobiont, Desulfobulbus oralis
journal, March 2018
- Cross, Karissa L.; Chirania, Payal; Xiong, Weili
- mBio, Vol. 9, Issue 2
Microbial Community Composition and Putative Biogeochemical Functions in the Sediment and Water of Tropical Granite Quarry Lakes
journal, May 2018
- Kumar, Amit; Ng, Daphne H. P.; Wu, Yichao
- Microbial Ecology, Vol. 77, Issue 1
Insights into the Evolution of Host Association through the Isolation and Characterization of a Novel Human Periodontal Pathobiont, Desulfobulbus oralis
journal, March 2018
- Cross, Karissa L.; Chirania, Payal; Xiong, Weili
- mBio, Vol. 9, Issue 2
A Ferredoxin- and F 420 H 2 -Dependent, Electron-Bifurcating, Heterodisulfide Reductase with Homologs in the Domains Bacteria and Archaea
journal, February 2017
- Yan, Zhen; Wang, Mingyu; Ferry, James G.
- mBio, Vol. 8, Issue 1
Flavin-Based Electron Bifurcation, Ferredoxin, Flavodoxin, and Anaerobic Respiration With Protons (Ech) or NAD+ (Rnf) as Electron Acceptors: A Historical Review
journal, March 2018
- Buckel, Wolfgang; Thauer, Rudolf K.
- Frontiers in Microbiology, Vol. 9
Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.