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Title: Physiological and proteomic analyses of Fe(III)-reducing co-cultures of Desulfotomaculum reducens MI-1 and Geobacter sulfurreducens PCA

Abstract

We established Fe(III)-reducing co-cultures of two species of metal-reducing bacteria, the Gram-positive Desulfotomaculum reducens MI-1 and the Gram-negative Geobacter sulfurreducens PCA. Co-cultures were given pyruvate, a substrate that D. reducens can ferment and use as electron donor for Fe(III) reduction. G. sulfurreducens, which does not utilize pyruvate, relied upon products of pyruvate oxidation by D. reducens (acetate, H2) for use as electron donor in the co-culture. Fe(III) was reduced more quickly in co-cultures relative to pure cultures, and powder X-ray diffraction evidence suggests that both organisms contribute to Fe(III) reduction. Global comparative proteomic analysis was performed in order to observe differential protein abundance during co-culture versus pure culture growth. Proteins previously associated with Fe(III) reduction in G. sulfurreducens, namely c-type cytochromes and type IV pili proteins, were significantly increased in abundance in co-cultures relative to pure cultures. Dreducens ribosomal proteins were significantly increased in co-cultures, likely a reflection of faster growth rates observed for D. reducens cells while in co-culture. Furthermore, we developed multiple reaction monitoring (MRM) assays to quantitate specific biomarker peptides. The assays were validated in pure and co-cultures, and protein abundance ratios from targeted MRM and global proteomic analysis correlate strongly.

Authors:
 [1];  [2];  [1];  [1];  [1]; ORCiD logo [2];  [1]
  1. Cornell University
  2. BATTELLE (PACIFIC NW LAB)
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1601805
Report Number(s):
PNNL-SA-119438
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Geobiology
Additional Journal Information:
Journal Volume: 16; Journal Issue: 5
Country of Publication:
United States
Language:
English

Citation Formats

Otwell, Annie E., Callister, Stephen J., Sherwood, Roberts, Zhang, Sheng, Goldman, Abby R., Smith, Richard D., and Richardson, Ruth E. Physiological and proteomic analyses of Fe(III)-reducing co-cultures of Desulfotomaculum reducens MI-1 and Geobacter sulfurreducens PCA. United States: N. p., 2018. Web. doi:10.1111/gbi.12295.
Otwell, Annie E., Callister, Stephen J., Sherwood, Roberts, Zhang, Sheng, Goldman, Abby R., Smith, Richard D., & Richardson, Ruth E. Physiological and proteomic analyses of Fe(III)-reducing co-cultures of Desulfotomaculum reducens MI-1 and Geobacter sulfurreducens PCA. United States. doi:10.1111/gbi.12295.
Otwell, Annie E., Callister, Stephen J., Sherwood, Roberts, Zhang, Sheng, Goldman, Abby R., Smith, Richard D., and Richardson, Ruth E. Sat . "Physiological and proteomic analyses of Fe(III)-reducing co-cultures of Desulfotomaculum reducens MI-1 and Geobacter sulfurreducens PCA". United States. doi:10.1111/gbi.12295.
@article{osti_1601805,
title = {Physiological and proteomic analyses of Fe(III)-reducing co-cultures of Desulfotomaculum reducens MI-1 and Geobacter sulfurreducens PCA},
author = {Otwell, Annie E. and Callister, Stephen J. and Sherwood, Roberts and Zhang, Sheng and Goldman, Abby R. and Smith, Richard D. and Richardson, Ruth E.},
abstractNote = {We established Fe(III)-reducing co-cultures of two species of metal-reducing bacteria, the Gram-positive Desulfotomaculum reducens MI-1 and the Gram-negative Geobacter sulfurreducens PCA. Co-cultures were given pyruvate, a substrate that D. reducens can ferment and use as electron donor for Fe(III) reduction. G. sulfurreducens, which does not utilize pyruvate, relied upon products of pyruvate oxidation by D. reducens (acetate, H2) for use as electron donor in the co-culture. Fe(III) was reduced more quickly in co-cultures relative to pure cultures, and powder X-ray diffraction evidence suggests that both organisms contribute to Fe(III) reduction. Global comparative proteomic analysis was performed in order to observe differential protein abundance during co-culture versus pure culture growth. Proteins previously associated with Fe(III) reduction in G. sulfurreducens, namely c-type cytochromes and type IV pili proteins, were significantly increased in abundance in co-cultures relative to pure cultures. Dreducens ribosomal proteins were significantly increased in co-cultures, likely a reflection of faster growth rates observed for D. reducens cells while in co-culture. Furthermore, we developed multiple reaction monitoring (MRM) assays to quantitate specific biomarker peptides. The assays were validated in pure and co-cultures, and protein abundance ratios from targeted MRM and global proteomic analysis correlate strongly.},
doi = {10.1111/gbi.12295},
journal = {Geobiology},
number = 5,
volume = 16,
place = {United States},
year = {2018},
month = {9}
}

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