Physiological and proteomic analyses of Fe(III)-reducing co-cultures of Desulfotomaculum reducens MI-1 and Geobacter sulfurreducens PCA

Otwell, Annie E.; Callister, Stephen J.; Sherwood, Roberts; Zhang, Sheng; Goldman, Abby R.; Smith, Richard D.; Richardson, Ruth E.

doi:10.1111/gbi.12295

Title: Physiological and proteomic analyses of Fe(III)-reducing co-cultures of Desulfotomaculum reducens MI-1 and Geobacter sulfurreducens PCA

Journal Article · Sat Sep 01 00:00:00 EDT 2018 · Geobiology

DOI:https://doi.org/10.1111/gbi.12295· OSTI ID:1601805

Otwell, Annie E. ^[1]; Callister, Stephen J. ^[2]; Sherwood, Roberts ^[1]; Zhang, Sheng ^[1]; Goldman, Abby R. ^[1];

^[2]; Richardson, Ruth E. ^[1]

Cornell University
BATTELLE (PACIFIC NW LAB)

We established Fe(III)-reducing co-cultures of two species of metal-reducing bacteria, the Gram-positive Desulfotomaculum reducens MI-1 and the Gram-negative Geobacter sulfurreducens PCA. Co-cultures were given pyruvate, a substrate that D. reducens can ferment and use as electron donor for Fe(III) reduction. G. sulfurreducens, which does not utilize pyruvate, relied upon products of pyruvate oxidation by D. reducens (acetate, H2) for use as electron donor in the co-culture. Fe(III) was reduced more quickly in co-cultures relative to pure cultures, and powder X-ray diffraction evidence suggests that both organisms contribute to Fe(III) reduction. Global comparative proteomic analysis was performed in order to observe differential protein abundance during co-culture versus pure culture growth. Proteins previously associated with Fe(III) reduction in G. sulfurreducens, namely c-type cytochromes and type IV pili proteins, were significantly increased in abundance in co-cultures relative to pure cultures. Dreducens ribosomal proteins were significantly increased in co-cultures, likely a reflection of faster growth rates observed for D. reducens cells while in co-culture. Furthermore, we developed multiple reaction monitoring (MRM) assays to quantitate specific biomarker peptides. The assays were validated in pure and co-cultures, and protein abundance ratios from targeted MRM and global proteomic analysis correlate strongly.

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Research Organization:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-76RL01830

OSTI ID:: 1601805

Report Number(s):: PNNL-SA-119438

Journal Information:: Geobiology, Vol. 16, Issue 5

Country of Publication:: United States

Language:: English

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