Clostridium thermocellum DSM 1313 transcriptional responses to redox perturbation

doi:10.1186/s13068-015-0394-9

Title: Clostridium thermocellum DSM 1313 transcriptional responses to redox perturbation

Clostridium thermocellum is a promising consolidated bioprocessing candidate organism capable of directly converting lignocellulosic biomass to ethanol. Current ethanol yields, productivities, and growth inhibitions are industrial deployment impediments for commodity fuel production by this bacterium. Redox imbalance under certain conditions and in engineered strains may contribute to incomplete substrate utilization and may direct fermentation products to undesirable overflow metabolites. As a result, towards a better understanding of redox metabolism in C. thermocellum, we established continuous growth conditions and analyzed global gene expression during addition of two stress chemicals (methyl viologen and hydrogen peroxide) which changed the fermentation redox potential.

Authors:

Sander, Kyle B. ^[1] ; Wilson, Charlotte M. ^[2] ; Rodriquez, Jr., Miguel ^[2] ; Klingeman, Dawn Marie ^[2] ; Davison, Brian H. ^[1] ; Brown, Steven D. ^[1] ; Rydzak, Thomas ^[2]

Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Publication Date:: 2015-12-12

Grant/Contract Number:: AC05-00OR22725

Type:: Accepted Manuscript

Journal Name:: Biotechnology for Biofuels

Additional Journal Information:: Journal Volume: 8; Journal Issue: 1; Journal ID: ISSN 1754-6834

Publisher:: BioMed Central

Research Org:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Org:: USDOE Office of Science (SC)

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; Clostridium thermocellum DSM 1313; microarray; transcriptomics; methyl viologen; chemostat; redox; sulfate; GS-GOGAT; hydrogenase

OSTI Identifier:: 1238009


                    Sander, Kyle B., Wilson, Charlotte M., Rodriquez, Jr., Miguel, Klingeman, Dawn Marie, Davison, Brian H., Brown, Steven D., and Rydzak, Thomas. Clostridium thermocellum DSM 1313 transcriptional responses to redox perturbation.  United States: N. p.,  
        Web.  doi:10.1186/s13068-015-0394-9.

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                    Sander, Kyle B., Wilson, Charlotte M., Rodriquez, Jr., Miguel, Klingeman, Dawn Marie, Davison, Brian H., Brown, Steven D., & Rydzak, Thomas. Clostridium thermocellum DSM 1313 transcriptional responses to redox perturbation.  United States.  doi:10.1186/s13068-015-0394-9.

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                    Sander, Kyle B., Wilson, Charlotte M., Rodriquez, Jr., Miguel, Klingeman, Dawn Marie, Davison, Brian H., Brown, Steven D., and Rydzak, Thomas. 2015.  
        "Clostridium thermocellum DSM 1313 transcriptional responses to redox perturbation".  United States.  
        doi:10.1186/s13068-015-0394-9.  https://www.osti.gov/servlets/purl/1238009.

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@article{osti_1238009,

  title        = {Clostridium thermocellum DSM 1313 transcriptional responses to redox perturbation},

  author       = {Sander, Kyle B. and Wilson, Charlotte M. and Rodriquez, Jr., Miguel and Klingeman, Dawn Marie and Davison, Brian H. and Brown, Steven D. and Rydzak, Thomas},

  abstractNote = {Clostridium thermocellum is a promising consolidated bioprocessing candidate organism capable of directly converting lignocellulosic biomass to ethanol. Current ethanol yields, productivities, and growth inhibitions are industrial deployment impediments for commodity fuel production by this bacterium. Redox imbalance under certain conditions and in engineered strains may contribute to incomplete substrate utilization and may direct fermentation products to undesirable overflow metabolites. As a result, towards a better understanding of redox metabolism in C. thermocellum, we established continuous growth conditions and analyzed global gene expression during addition of two stress chemicals (methyl viologen and hydrogen peroxide) which changed the fermentation redox potential.},

  doi          = {10.1186/s13068-015-0394-9},

  journal      = {Biotechnology for Biofuels},

  number       = 1,

  volume       = 8,

  place        = {United States},

  year         = {2015},

  month        = {12}

}

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Accepted Manuscript (DOE)
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10.1186/s13068-015-0394-9
https://doi.org/10.1186/s13068-015-0394-9

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Works referenced in this record:

Consolidated bioprocessing of cellulosic biomass: an update
journal, October 2005

Lynd, Lee R.; van Zyl, Willem H.; McBride, John E.
Current Opinion in Biotechnology, Vol. 16, Issue 5, p. 577-583
DOI: 10.1016/j.copbio.2005.08.009

Redirecting carbon flux through exogenous pyruvate kinase to achieve high ethanol yields in Clostridium thermocellum
journal, January 2013

Deng, Yu; Olson, Daniel G.; Zhou, Jilai
Metabolic Engineering, Vol. 15, p. 151-158
DOI: 10.1016/j.ymben.2012.11.006

High Ethanol Titers from Cellulose by Using Metabolically Engineered Thermophilic, Anaerobic Microbes
journal, September 2011

Argyros, D. Aaron; Tripathi, Shital A.; Barrett, Trisha F.
Applied and Environmental Microbiology, Vol. 77, Issue 23, p. 8288-8294
DOI: 10.1128/AEM.00646-11

Development of pyrF-Based Genetic System for Targeted Gene Deletion in Clostridium thermocellum and Creation of a pta Mutant
journal, August 2010

Tripathi, S. A.; Olson, D. G.; Argyros, D. A.
Applied and Environmental Microbiology, Vol. 76, Issue 19, p. 6591-6599
DOI: 10.1128/AEM.01484-10

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Title: Clostridium thermocellum DSM 1313 transcriptional responses to redox perturbation

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