Clostridium thermocellum DSM 1313 transcriptional responses to redox perturbation

Sander, Kyle; Wilson, Charlotte M.; Rodriguez, Jr., Miguel; Klingeman, Dawn M.; Rydzak, Thomas; Davison, Brian H.; Brown, Steven D.

doi:10.1186/s13068-015-0394-9

Title: Clostridium thermocellum DSM 1313 transcriptional responses to redox perturbation

Abstract

Clostridium thermocellum is a promising consolidated bioprocessing candidate organism capable of directly converting lignocellulosic biomass to ethanol. Current ethanol yields, productivities, and growth inhibitions are industrial deployment impediments for commodity fuel production by this bacterium. Redox imbalance under certain conditions and in engineered strains may contribute to incomplete substrate utilization and may direct fermentation products to undesirable overflow metabolites. As a result, towards a better understanding of redox metabolism in C. thermocellum, we established continuous growth conditions and analyzed global gene expression during addition of two stress chemicals (methyl viologen and hydrogen peroxide) which changed the fermentation redox potential.

Authors:: Sander, Kyle; Wilson, Charlotte M.; Rodriguez, Jr., Miguel; Klingeman, Dawn M.; Rydzak, Thomas; Davison, Brian H.; Brown, Steven D.

Publication Date:: Sat Dec 12 00:00:00 EST 2015

Research Org.:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:: USDOE Office of Science (SC)

OSTI Identifier:: 1618631

Alternate Identifier(s):: OSTI ID: 1238009

Grant/Contract Number:: AC05-00OR22725

Resource Type:: Published Article

Journal Name:: Biotechnology for Biofuels

Additional Journal Information:: Journal Name: Biotechnology for Biofuels Journal Volume: 8 Journal Issue: 1; Journal ID: ISSN 1754-6834

Publisher:: Springer Science + Business Media

Country of Publication:: Netherlands

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; Clostridium thermocellum DSM 1313; microarray; transcriptomics; methyl viologen; chemostat; redox; sulfate; GS-GOGAT; hydrogenase

Citation Formats


                    Sander, Kyle, Wilson, Charlotte M., Rodriguez, Jr., Miguel, Klingeman, Dawn M., Rydzak, Thomas, Davison, Brian H., and Brown, Steven D. Clostridium thermocellum DSM 1313 transcriptional responses to redox perturbation.  Netherlands: N. p., 2015. 
Web.  doi:10.1186/s13068-015-0394-9.

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                    Sander, Kyle, Wilson, Charlotte M., Rodriguez, Jr., Miguel, Klingeman, Dawn M., Rydzak, Thomas, Davison, Brian H., & Brown, Steven D. Clostridium thermocellum DSM 1313 transcriptional responses to redox perturbation.  Netherlands.  https://doi.org/10.1186/s13068-015-0394-9

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                    Sander, Kyle, Wilson, Charlotte M., Rodriguez, Jr., Miguel, Klingeman, Dawn M., Rydzak, Thomas, Davison, Brian H., and Brown, Steven D. Sat .  
"Clostridium thermocellum DSM 1313 transcriptional responses to redox perturbation".  Netherlands.  https://doi.org/10.1186/s13068-015-0394-9.

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@article{osti_1618631,

  title        = {Clostridium thermocellum DSM 1313 transcriptional responses to redox perturbation},

  author       = {Sander, Kyle and Wilson, Charlotte M. and Rodriguez, Jr., Miguel and Klingeman, Dawn M. and Rydzak, Thomas and Davison, Brian H. and Brown, Steven D.},

  abstractNote = {Clostridium thermocellum is a promising consolidated bioprocessing candidate organism capable of directly converting lignocellulosic biomass to ethanol. Current ethanol yields, productivities, and growth inhibitions are industrial deployment impediments for commodity fuel production by this bacterium. Redox imbalance under certain conditions and in engineered strains may contribute to incomplete substrate utilization and may direct fermentation products to undesirable overflow metabolites. As a result, towards a better understanding of redox metabolism in C. thermocellum, we established continuous growth conditions and analyzed global gene expression during addition of two stress chemicals (methyl viologen and hydrogen peroxide) which changed the fermentation redox potential.},

  doi          = {10.1186/s13068-015-0394-9},

  journal      = {Biotechnology for Biofuels},

  number       = 1,

  volume       = 8,

  place        = {Netherlands},

  year         = {Sat Dec 12 00:00:00 EST 2015},

  month        = {Sat Dec 12 00:00:00 EST 2015}

}

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Cited by: 18 works

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Fig. 1: Chemostat growth and redox potential before, during, and after methyl viologen addition. a Over 280 h and b for detailed view of boxed region indicated in panel a

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