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Title: Anaerobic microplate assay for direct microbial conversion of switchgrass and Avicel using Clostridium thermocellum

Clostridium thermocellum parent ..delta..hpt strain deconstructed Avicel to cellobiose, glucose, and generated lactic acid, formic acid, acetic acid and ethanol as fermentation products in titers and ratios similar to larger scale fermentations confirming the suitability of a plate-based method for C. thermocellum growth studies. C. thermocellum strain LL1210, with gene deletions in the key central metabolic pathways, produced higher ethanol titers in the Consolidated Bioprocessing (CBP) plate assay for both Avicel and switchgrass fermentations when compared to the ..delta..hpt strain.
Authors:
; ; ; ; ; ;
Publication Date:
Report Number(s):
NREL/JA-2700-70541
Journal ID: ISSN 0141-5492
Grant/Contract Number:
AC36-08GO28308
Type:
Published Article
Journal Name:
Biotechnology Letters
Additional Journal Information:
Journal Volume: 40; Journal Issue: 2; Journal ID: ISSN 0141-5492
Publisher:
Springer
Research Org:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; bioethanol; consolidated bioprocessing; high throughput screening; lignocellulosic biomass; microplate assay
OSTI Identifier:
1408146
Alternate Identifier(s):
OSTI ID: 1409731

Oguntimein, Gbekeloluwa B., Rodriguez, Miguel, Dumitrache, Alexandru, Shollenberger, Todd, Decker, Stephen R., Davison, Brian H., and Brown, Steven D.. Anaerobic microplate assay for direct microbial conversion of switchgrass and Avicel using Clostridium thermocellum. United States: N. p., Web. doi:10.1007/s10529-017-2467-2.
Oguntimein, Gbekeloluwa B., Rodriguez, Miguel, Dumitrache, Alexandru, Shollenberger, Todd, Decker, Stephen R., Davison, Brian H., & Brown, Steven D.. Anaerobic microplate assay for direct microbial conversion of switchgrass and Avicel using Clostridium thermocellum. United States. doi:10.1007/s10529-017-2467-2.
Oguntimein, Gbekeloluwa B., Rodriguez, Miguel, Dumitrache, Alexandru, Shollenberger, Todd, Decker, Stephen R., Davison, Brian H., and Brown, Steven D.. 2017. "Anaerobic microplate assay for direct microbial conversion of switchgrass and Avicel using Clostridium thermocellum". United States. doi:10.1007/s10529-017-2467-2.
@article{osti_1408146,
title = {Anaerobic microplate assay for direct microbial conversion of switchgrass and Avicel using Clostridium thermocellum},
author = {Oguntimein, Gbekeloluwa B. and Rodriguez, Miguel and Dumitrache, Alexandru and Shollenberger, Todd and Decker, Stephen R. and Davison, Brian H. and Brown, Steven D.},
abstractNote = {Clostridium thermocellum parent ..delta..hpt strain deconstructed Avicel to cellobiose, glucose, and generated lactic acid, formic acid, acetic acid and ethanol as fermentation products in titers and ratios similar to larger scale fermentations confirming the suitability of a plate-based method for C. thermocellum growth studies. C. thermocellum strain LL1210, with gene deletions in the key central metabolic pathways, produced higher ethanol titers in the Consolidated Bioprocessing (CBP) plate assay for both Avicel and switchgrass fermentations when compared to the ..delta..hpt strain.},
doi = {10.1007/s10529-017-2467-2},
journal = {Biotechnology Letters},
number = 2,
volume = 40,
place = {United States},
year = {2017},
month = {11}
}

Works referenced in this record:

High Ethanol Titers from Cellulose by Using Metabolically Engineered Thermophilic, Anaerobic Microbes
journal, September 2011
  • Argyros, D. Aaron; Tripathi, Shital A.; Barrett, Trisha F.
  • Applied and Environmental Microbiology, Vol. 77, Issue 23, p. 8288-8294
  • DOI: 10.1128/AEM.00646-11