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Title: Lipidomic Analysis of Endocannabinoid Signaling: Targeted Metabolite Identification and Quantification

The endocannabinoidsN-arachidonoylethanolamide (or anandamide, AEA) and 2-arachidonoylglycerol (2-AG) belong to the larger groups ofN-acylethanolamines (NAEs) and monoacylglycerol (MAG) lipid classes, respectively. They are biologically active lipid molecules that activate G-protein-coupled cannabinoid receptors found in various organisms. After AEA and 2-AG were discovered in the 1990s, they have been extensively documented to have a broad range of physiological functions. Along with AEA, several NAEs, for example,N-palmitoylethanolamine (PEA),N-stearoylethanolamine (SEA), andN-oleoylethanolamine (OEA) are also present in tissues, usually at much larger concentrations than AEA. Any perturbation that involves the endocannabinoid pathway may subsequently alter basal level or metabolism of these lipid mediators. Further, the altered levels of these molecules often reflect pathological conditions associated with tissue damage. Robust and sensitive methodologies to analyze these lipid mediators are essential to understanding how they act as endocannabinoids. Lastly, the recent advances in mass spectrometry allow researchers to develop lipidomics approaches and several methodologies have been proposed to quantify endocannabinoids in various biological systems.
Authors:
 [1] ;  [2]
  1. Univ. of North Texas, Denton, TX (United States); Brookhaven National Lab. (BNL), Upton, NY (United States)
  2. Univ. of North Texas, Denton, TX (United States)
Publication Date:
Report Number(s):
BNL-112088-2016-JA
Journal ID: ISSN 2090-5904; PII: 2426398; 2426398
Grant/Contract Number:
FG02-05ER15647; SC00112704; FG02- 05ER15647
Type:
Published Article
Journal Name:
Neural Plasticity
Additional Journal Information:
Journal Volume: 2016; Journal ID: ISSN 2090-5904
Publisher:
Hindawi
Research Org:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES
OSTI Identifier:
1234050
Alternate Identifier(s):
OSTI ID: 1263472; OSTI ID: 1335430