Continuous Succinic Acid Production by Actinobacillus succinogenes on Xylose-Enriched Hydrolysate

doi:10.1186/s13068-015-0363-3

Title: Continuous Succinic Acid Production by Actinobacillus succinogenes on Xylose-Enriched Hydrolysate

Bio-manufacturing of high-value chemicals in parallel to renewable biofuels has the potential to dramatically improve the overall economic landscape of integrated lignocellulosic biorefineries. However, this will require the generation of carbohydrate streams from lignocellulose in a form suitable for efficient microbial conversion and downstream processing appropriate to the desired end use, making overall process development, along with selection of appropriate target molecules, crucial to the integrated biorefinery. Succinic acid (SA), a high-value target molecule, can be biologically produced from sugars and has the potential to serve as a platform chemical for various chemical and polymer applications. However, the feasibility of microbial SA production at industrially relevant productivities and yields from lignocellulosic biorefinery streams has not yet been reported.

Authors:

Bradfield, Michael F. A. ^[1] ; Mohagheghi, Ali ^[2] ; Salvachua, Davinia ^[2] ; Smith, Holly ^[2] ; Black, Brenna A. ^[2] ; Dowe, Nancy ^[2] ; Beckham, Gregg T. ^[2] ; Nicol, Willie ^[3]

Univ. of Pretoria, Hatfield (South Africa); National Renewable Energy Lab. (NREL), Golden, CO (United States)
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Univ. of Pretoria, Hatfield (South Africa)

Publication Date:: 2015-11-14

Report Number(s):: NREL/JA-5100-65596
Journal ID: ISSN 1754-6834

Grant/Contract Number:: AC36-08GO28308

Type:: Accepted Manuscript

Journal Name:: Biotechnology for Biofuels

Additional Journal Information:: Journal Volume: 8; Related Information: Biotechnology for Biofuels; Journal ID: ISSN 1754-6834

Publisher:: BioMed Central

Research Org:: National Renewable Energy Lab. (NREL), Golden, CO (United States)

Sponsoring Org:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)

Country of Publication:: United States

Language:: English

Subject:: 09 BIOMASS FUELS; biorefinery; Actinobacillus succinogenes; succinic acid; continuous fermentation; corn stover hydrolysates

OSTI Identifier:: 1233689


                    Bradfield, Michael F. A., Mohagheghi, Ali, Salvachua, Davinia, Smith, Holly, Black, Brenna A., Dowe, Nancy, Beckham, Gregg T., and Nicol, Willie. Continuous Succinic Acid Production by Actinobacillus succinogenes on Xylose-Enriched Hydrolysate.  United States: N. p.,  
        Web.  doi:10.1186/s13068-015-0363-3.

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                    Bradfield, Michael F. A., Mohagheghi, Ali, Salvachua, Davinia, Smith, Holly, Black, Brenna A., Dowe, Nancy, Beckham, Gregg T., & Nicol, Willie. Continuous Succinic Acid Production by Actinobacillus succinogenes on Xylose-Enriched Hydrolysate.  United States.  doi:10.1186/s13068-015-0363-3.

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                    Bradfield, Michael F. A., Mohagheghi, Ali, Salvachua, Davinia, Smith, Holly, Black, Brenna A., Dowe, Nancy, Beckham, Gregg T., and Nicol, Willie. 2015.  
        "Continuous Succinic Acid Production by Actinobacillus succinogenes on Xylose-Enriched Hydrolysate".  United States.  
        doi:10.1186/s13068-015-0363-3.  https://www.osti.gov/servlets/purl/1233689.

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@article{osti_1233689,

  title        = {Continuous Succinic Acid Production by Actinobacillus succinogenes on Xylose-Enriched Hydrolysate},

  author       = {Bradfield, Michael F. A. and Mohagheghi, Ali and Salvachua, Davinia and Smith, Holly and Black, Brenna A. and Dowe, Nancy and Beckham, Gregg T. and Nicol, Willie},

  abstractNote = {Bio-manufacturing of high-value chemicals in parallel to renewable biofuels has the potential to dramatically improve the overall economic landscape of integrated lignocellulosic biorefineries. However, this will require the generation of carbohydrate streams from lignocellulose in a form suitable for efficient microbial conversion and downstream processing appropriate to the desired end use, making overall process development, along with selection of appropriate target molecules, crucial to the integrated biorefinery. Succinic acid (SA), a high-value target molecule, can be biologically produced from sugars and has the potential to serve as a platform chemical for various chemical and polymer applications. However, the feasibility of microbial SA production at industrially relevant productivities and yields from lignocellulosic biorefinery streams has not yet been reported.},

  doi          = {10.1186/s13068-015-0363-3},

  journal      = {Biotechnology for Biofuels},

  number       = ,

  volume       = 8,

  place        = {United States},

  year         = {2015},

  month        = {11}

}

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Accepted Manuscript (DOE)
Publisher's Version of Record
10.1186/s13068-015-0363-3
https://doi.org/10.1186/s13068-015-0363-3

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Works referenced in this record:

Dilute-Sulfuric Acid Pretreatment of Corn Stover in Pilot-Scale Reactor: Investigation of Yields, Kinetics, and Enzymatic Digestibilities of Solids
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Schell, Daniel J.; Farmer, Jody; Newman, Millie
Applied Biochemistry and Biotechnology, Vol. 105, Issue 1-3, p. 69-86
DOI: 10.1385/ABAB:105:1-3:69

A new process for the continuous production of succinic acid from glucose at high yield, titer, and productivity
journal, January 2007

Meynial-Salles, Isabelle; Dorotyn, Sophie; Soucaille, Philippe
Biotechnology and Bioengineering, Vol. 99, Issue 1, p. 129-135
DOI: 10.1002/bit.21521

Fermentation of lignocellulosic hydrolysates. I: inhibition and detoxification
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Palmqvist, Eva; Hahn-Hägerdal, Bärbel
Bioresource Technology, Vol. 74, Issue 1, p. 17-24
DOI: 10.1016/S0960-8524(99)00160-1

Deconstruction of Lignocellulosic Biomass to Fuels and Chemicals
journal, July 2011

Chundawat, Shishir P. S.; Beckham, Gregg T.; Himmel, Michael E.
Annual Review of Chemical and Biomolecular Engineering, Vol. 2, Issue 1, p. 121-145
DOI: 10.1146/annurev-chembioeng-061010-114205

Succinic Acid: A New Platform Chemical for Biobased Polymers from Renewable Resources
journal, May 2008

Bechthold, I.; Bretz, K.; Kabasci, S.
Chemical Engineering & Technology, Vol. 31, Issue 5, p. 647-654
DOI: 10.1002/ceat.200800063

Combining metabolic engineering and metabolic evolution to develop nonrecombinant strains of Escherichia coli C that produce succinate and malate
journal, January 2008

Jantama, Kaemwich; Haupt, M. J.; Svoronos, Spyros A.
Biotechnology and Bioengineering, Vol. 99, Issue 5, p. 1140-1153
DOI: 10.1002/bit.21694

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