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Title: Succinic acid production from lignocellulosic hydrolysate by Basfia succiniciproducens

The production of chemicals alongside fuels will be essential to enhance the feasibility of lignocellulosic biorefineries. Succinic acid (SA), a naturally occurring C4-diacid, is a primary intermediate of the tricarboxylic acid cycle and a promising building block chemical that has received significant industrial attention. Basfia succiniciproducens is a relatively unexplored SA-producing bacterium with advantageous features such as broad substrate utilization, genetic tractability, and facultative anaerobic metabolism. Here B. succiniciproducens is evaluated in high xylose-content hydrolysates from corn stover and different synthetic media in batch fermentation. SA titers in hydrolysate at an initial sugar concentration of 60 g/L reached up to 30 g/L, with metabolic yields of 0.69 g/g, and an overall productivity of 0.43 g/L/h. These results demonstrate that B. succiniciproducens may be an attractive platform organism for bio-SA production from biomass hydrolysates.
Authors:
 [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [1] ;  [1]
  1. National Renewable Energy Lab. (NREL), Golden, CO (United States). National Bioenergy Center
Publication Date:
Report Number(s):
NREL/JA-5100-66481
Journal ID: ISSN 0960-8524
Grant/Contract Number:
AC36-08GO28308
Type:
Accepted Manuscript
Journal Name:
Bioresource Technology
Additional Journal Information:
Journal Volume: 214; Journal Issue: C; Journal ID: ISSN 0960-8524
Publisher:
Elsevier
Research Org:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; biorefinery; biochemical; corn stover; xylose; Actinobacillus succinogenes
OSTI Identifier:
1254113
Alternate Identifier(s):
OSTI ID: 1326455

Salvachúa, Davinia, Smith, Holly, St. John, Peter C., Mohagheghi, Ali, Peterson, Darren J., Black, Brenna A., Dowe, Nancy, and Beckham, Gregg T.. Succinic acid production from lignocellulosic hydrolysate by Basfia succiniciproducens. United States: N. p., Web. doi:10.1016/j.biortech.2016.05.018.
Salvachúa, Davinia, Smith, Holly, St. John, Peter C., Mohagheghi, Ali, Peterson, Darren J., Black, Brenna A., Dowe, Nancy, & Beckham, Gregg T.. Succinic acid production from lignocellulosic hydrolysate by Basfia succiniciproducens. United States. doi:10.1016/j.biortech.2016.05.018.
Salvachúa, Davinia, Smith, Holly, St. John, Peter C., Mohagheghi, Ali, Peterson, Darren J., Black, Brenna A., Dowe, Nancy, and Beckham, Gregg T.. 2016. "Succinic acid production from lignocellulosic hydrolysate by Basfia succiniciproducens". United States. doi:10.1016/j.biortech.2016.05.018. https://www.osti.gov/servlets/purl/1254113.
@article{osti_1254113,
title = {Succinic acid production from lignocellulosic hydrolysate by Basfia succiniciproducens},
author = {Salvachúa, Davinia and Smith, Holly and St. John, Peter C. and Mohagheghi, Ali and Peterson, Darren J. and Black, Brenna A. and Dowe, Nancy and Beckham, Gregg T.},
abstractNote = {The production of chemicals alongside fuels will be essential to enhance the feasibility of lignocellulosic biorefineries. Succinic acid (SA), a naturally occurring C4-diacid, is a primary intermediate of the tricarboxylic acid cycle and a promising building block chemical that has received significant industrial attention. Basfia succiniciproducens is a relatively unexplored SA-producing bacterium with advantageous features such as broad substrate utilization, genetic tractability, and facultative anaerobic metabolism. Here B. succiniciproducens is evaluated in high xylose-content hydrolysates from corn stover and different synthetic media in batch fermentation. SA titers in hydrolysate at an initial sugar concentration of 60 g/L reached up to 30 g/L, with metabolic yields of 0.69 g/g, and an overall productivity of 0.43 g/L/h. These results demonstrate that B. succiniciproducens may be an attractive platform organism for bio-SA production from biomass hydrolysates.},
doi = {10.1016/j.biortech.2016.05.018},
journal = {Bioresource Technology},
number = C,
volume = 214,
place = {United States},
year = {2016},
month = {5}
}