Biotransformation of furanic and phenolic compounds with hydrogen gas production in a microbial electrolysis cell
Abstract
In this study, furanic and phenolic compounds are problematic byproducts resulting from the decomposition of lignocellulosic biomass during biofuel production. This study assessed the capacity of a microbial electrolysis cell (MEC) to produce hydrogen gas (H2) using a mixture of two furanic (furfural, FF; 5-hydroxymethyl furfural, HMF) and three phenolic (syringic acid, SA; vanillic acid, VA; and 4-hydroxybenzoic acid, HBA) compounds as the sole carbon and energy source in the bioanode. The rate and extent of biotransformation of the five compounds, efficiency of H2 production, as well as the anode microbial community structure were investigated. The five compounds were completely transformed within 7-day batch runs and their biotransformation rate increased with increasing initial concentration. At an initial concentration of 1,200 mg/L (8.7 mM) of the mixture of the five compounds, their biotransformation rate ranged from 0.85 to 2.34 mM/d. The anode coulombic efficiency was 44-69%, which is comparable to wastewater-fed MECs. The H2 yield varied from 0.26 to 0.42 g H2-COD/g COD removed in the anode, and the bioanode volume-normalized H2 production rate was 0.07-0.1 L/L-d. The major identified fermentation products that did not transform further were catechol and phenol. Acetate was the direct substrate for exoelectrogenesis. Current and H2more »
- Authors:
-
- Georgia Inst. of Technology, Atlanta, GA (United States)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); The Univ. of Tennessee, Knoxville, TN (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE)
- OSTI Identifier:
- 1225423
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Environmental Science and Technology
- Additional Journal Information:
- Journal Name: Environmental Science and Technology; Journal ID: ISSN 0013-936X
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; 08 HYDROGEN; microbial electrolysis; bioelectrochemical systems; hydrogen
Citation Formats
Zeng, Xiaofei, Borole, Abhijeet P., and Pavlostathis, Spyros G. Biotransformation of furanic and phenolic compounds with hydrogen gas production in a microbial electrolysis cell. United States: N. p., 2015.
Web. doi:10.1021/acs.est.5b02313.
Zeng, Xiaofei, Borole, Abhijeet P., & Pavlostathis, Spyros G. Biotransformation of furanic and phenolic compounds with hydrogen gas production in a microbial electrolysis cell. United States. https://doi.org/10.1021/acs.est.5b02313
Zeng, Xiaofei, Borole, Abhijeet P., and Pavlostathis, Spyros G. Tue .
"Biotransformation of furanic and phenolic compounds with hydrogen gas production in a microbial electrolysis cell". United States. https://doi.org/10.1021/acs.est.5b02313. https://www.osti.gov/servlets/purl/1225423.
@article{osti_1225423,
title = {Biotransformation of furanic and phenolic compounds with hydrogen gas production in a microbial electrolysis cell},
author = {Zeng, Xiaofei and Borole, Abhijeet P. and Pavlostathis, Spyros G.},
abstractNote = {In this study, furanic and phenolic compounds are problematic byproducts resulting from the decomposition of lignocellulosic biomass during biofuel production. This study assessed the capacity of a microbial electrolysis cell (MEC) to produce hydrogen gas (H2) using a mixture of two furanic (furfural, FF; 5-hydroxymethyl furfural, HMF) and three phenolic (syringic acid, SA; vanillic acid, VA; and 4-hydroxybenzoic acid, HBA) compounds as the sole carbon and energy source in the bioanode. The rate and extent of biotransformation of the five compounds, efficiency of H2 production, as well as the anode microbial community structure were investigated. The five compounds were completely transformed within 7-day batch runs and their biotransformation rate increased with increasing initial concentration. At an initial concentration of 1,200 mg/L (8.7 mM) of the mixture of the five compounds, their biotransformation rate ranged from 0.85 to 2.34 mM/d. The anode coulombic efficiency was 44-69%, which is comparable to wastewater-fed MECs. The H2 yield varied from 0.26 to 0.42 g H2-COD/g COD removed in the anode, and the bioanode volume-normalized H2 production rate was 0.07-0.1 L/L-d. The major identified fermentation products that did not transform further were catechol and phenol. Acetate was the direct substrate for exoelectrogenesis. Current and H2 production were inhibited at an initial substrate concentration of 1,200 mg/L, resulting in acetate accumulation at a much higher level than that measured in other batch runs conducted with a lower initial concentration of the five compounds. The anode microbial community consisted of exoelectrogens, putative degraders of the five compounds, and syntrophic partners of exoelectrogens. The H2 production route demonstrated in this study has proven to be an alternative to the currently used process of reforming natural gas to supply H2 needed to upgrade bio-oils to stable hydrocarbon fuels.},
doi = {10.1021/acs.est.5b02313},
journal = {Environmental Science and Technology},
number = ,
volume = ,
place = {United States},
year = {Tue Oct 27 00:00:00 EDT 2015},
month = {Tue Oct 27 00:00:00 EDT 2015}
}
Web of Science
Works referenced in this record:
Essential Data and Techniques for Conducting Microbial Fuel Cell and other Types of Bioelectrochemical System Experiments
journal, April 2012
- Logan, Bruce E.
- ChemSusChem, Vol. 5, Issue 6
A monetary comparison of energy recovered from microbial fuel cells and microbial electrolysis cells fed winery or domestic wastewaters
journal, September 2010
- Cusick, Roland D.; Kiely, Patrick D.; Logan, Bruce E.
- International Journal of Hydrogen Energy, Vol. 35, Issue 17
Microbial Metabolism of the Plant Phenolic Compounds Ferulic and Syringic Acids under Three Anaerobic Conditions
journal, April 1997
- Phelps, C. D.; Young, L. Y.
- Microbial Ecology, Vol. 33, Issue 3
Reduced internal resistance of microbial electrolysis cell (MEC) as factors of configuration and stuffing with granular activated carbon
journal, December 2010
- Wang, Aijie; Liu, Wenzong; Ren, Nanqi
- International Journal of Hydrogen Energy, Vol. 35, Issue 24
Newly Cultured Bacteria with Broad Diversity Isolated from Eight-Week Continuous Culture Enrichments of Cow Feces on Complex Polysaccharides
journal, November 2013
- Ziemer, Cherie J.
- Applied and Environmental Microbiology, Vol. 80, Issue 2
Hydrogen Production in a Single Chamber Microbial Electrolysis Cell Lacking a Membrane
journal, May 2008
- Call, Douglas; Logan, Bruce E.
- Environmental Science & Technology, Vol. 42, Issue 9, p. 3401-3406
Inhibition of ethanol-producing yeast and bacteria by degradation products produced during pre-treatment of biomass
journal, August 2004
- Klinke, H. B.; Thomsen, A. B.; Ahring, B. K.
- Applied Microbiology and Biotechnology, Vol. 66, Issue 1, p. 10-26
Bioconversion of lignocellulosic biomass to hydrogen: Potential and challenges
journal, November 2009
- Ren, Nanqi; Wang, Aijie; Cao, Guangli
- Biotechnology Advances, Vol. 27, Issue 6
Metagenomic Analyses Reveal the Involvement of Syntrophic Consortia in Methanol/Electricity Conversion in Microbial Fuel Cells
journal, May 2014
- Yamamuro, Ayaka; Kouzuma, Atsushi; Abe, Takashi
- PLoS ONE, Vol. 9, Issue 5
The electric picnic: synergistic requirements for exoelectrogenic microbial communities
journal, June 2011
- Kiely, Patrick D.; Regan, John M.; Logan, Bruce E.
- Current Opinion in Biotechnology, Vol. 22, Issue 3
Oxidation of biomass derived 5-hydroxymethylfurfural using heterogeneous and electrochemical catalysis
journal, November 2012
- Vuyyuru, Koteswara Rao; Strasser, Peter
- Catalysis Today, Vol. 195, Issue 1, p. 144-154
Hydrogen Production with a Microbial Biocathode
December 2007
- Rozendal, René A.; Jeremiasse, Adriaan W.; Hamelers, Hubertus V. M.
- Environmental Science & Technology, Vol. 42, Issue 2, p. 629-634
Biological hydrogen production: prospects and challenges
journal, May 2010
- Lee, Hyung-Sool; Vermaas, Wim F. J.; Rittmann, Bruce E.
- Trends in Biotechnology, Vol. 28, Issue 5
Performance of a continuous flow microbial electrolysis cell (MEC) fed with domestic wastewater
journal, August 2012
- Escapa, A.; Gil-Carrera, L.; García, V.
- Bioresource Technology, Vol. 117
Do furanic and phenolic compounds of lignocellulosic and algae biomass hydrolyzate inhibit anaerobic mixed cultures? A comprehensive review
journal, September 2014
- Monlau, F.; Sambusiti, C.; Barakat, A.
- Biotechnology Advances, Vol. 32, Issue 5
Clostridium populeti sp. nov., a Cellulolytic Species from a Woody-Biomass Digestor
journal, April 1985
- Sleat, R.; Mah, R. A.
- International Journal of Systematic Bacteriology, Vol. 35, Issue 2
Microbial community structure in a biofilm anode fed with a fermentable substrate: The significance of hydrogen scavengers
journal, January 2010
- Parameswaran, Prathap; Zhang, Husen; Torres, César I.
- Biotechnology and Bioengineering, Vol. 105, Issue 1
Production of hydrogen from domestic wastewater using a bioelectrochemically assisted microbial reactor (BEAMR)
journal, September 2007
- Ditzig, J.; Liu, H.; Logan, B.
- International Journal of Hydrogen Energy, Vol. 32, Issue 13
Electrochemically Assisted Microbial Production of Hydrogen from Acetate
journal, June 2005
- Liu, Hong; Grot, Stephen; Logan, Bruce E.
- Environmental Science & Technology, Vol. 39, Issue 11
Microbial Fuel Cells—Challenges and Applications
journal, September 2006
- Logan, Bruce E.; Regan, John M.
- Environmental Science & Technology, Vol. 40, Issue 17
Enhanced current production by Desulfovibrio desulfuricans biofilm in a mediator-less microbial fuel cell
journal, August 2014
- Kang, Christina S.; Eaktasang, Numfon; Kwon, Dae-Young
- Bioresource Technology, Vol. 165
Long-term cathode performance and the microbial communities that develop in microbial fuel cells fed different fermentation endproducts
journal, January 2011
- Kiely, Patrick D.; Rader, Geoffrey; Regan, John M.
- Bioresource Technology, Vol. 102, Issue 1
Microbial degradation of furanic compounds: biochemistry, genetics, and impact
journal, October 2011
- Wierckx, Nick; Koopman, Frank; Ruijssenaars, Harald J.
- Applied Microbiology and Biotechnology, Vol. 92, Issue 6
Effects of furan derivatives and phenolic compounds on electricity generation in microbial fuel cells
journal, May 2008
- Catal, Tunc; Fan, Yanzhen; Li, Kaichang
- Journal of Power Sources, Vol. 180, Issue 1
Electrochemical investigations of the oxidation–reduction of furfural in aqueous medium
journal, January 2004
- Parpot, P.; Bettencourt, A. P.; Chamoulaud, G.
- Electrochimica Acta, Vol. 49, Issue 3, p. 397-403
Hydrogen production from switchgrass via an integrated pyrolysis–microbial electrolysis process
journal, November 2015
- Lewis, A. J.; Ren, S.; Ye, X.
- Bioresource Technology, Vol. 195
Group contributions for estimating standard gibbs energies of formation of biochemical compounds in aqueous solution
journal, December 1990
- Mavrovouniotis, Michael L.
- Biotechnology and Bioengineering, Vol. 36, Issue 10
Additional characteristics of one-carbon-compound utilization by Eubacterium limosum and Acetobacterium woodii.
journal, January 1987
- Sharak Genthner, B. R.; Bryant, M. P.
- Applied and Environmental Microbiology, Vol. 53, Issue 3
Inhibition of fermentative hydrogen production by lignocellulose-derived compounds in mixed cultures
journal, February 2012
- Quéméneur, Marianne; Hamelin, Jérôme; Barakat, Abdellatif
- International Journal of Hydrogen Energy, Vol. 37, Issue 4
Syntrophic Processes Drive the Conversion of Glucose in Microbial Fuel Cell Anodes
journal, November 2008
- Freguia, Stefano; Rabaey, Korneel; Yuan, Zhiguo
- Environmental Science & Technology, Vol. 42, Issue 21
Controlling accumulation of fermentation inhibitors in biorefinery recycle water using microbial fuel cells
journal, January 2009
- Borole, Abhijeet P.; Mielenz, Jonathan R.; Vishnivetskaya, Tatiana A.
- Biotechnology for Biofuels, Vol. 2, Issue 1
Geobacter anodireducens sp. nov., an exoelectrogenic microbe in bioelectrochemical systems
journal, October 2014
- Sun, Dan; Wang, Aijie; Cheng, Shaoan
- International Journal of Systematic and Evolutionary Microbiology, Vol. 64, Issue Pt_10
Effects of furan derivatives on biohydrogen fermentation from wet steam-exploded cornstalk and its microbial community
journal, January 2015
- Liu, Zhidan; Zhang, Chong; Wang, Linjun
- Bioresource Technology, Vol. 175
Anaerobic Degradation of Aromatic Compounds
journal, October 1988
- Evans, W. C.; Fuchs, G.
- Annual Review of Microbiology, Vol. 42, Issue 1
Electricity Generation Using an Air-Cathode Single Chamber Microbial Fuel Cell in the Presence and Absence of a Proton Exchange Membrane
journal, July 2004
- Liu, Hong; Logan, Bruce E.
- Environmental Science & Technology, Vol. 38, Issue 14
Features of rumen and sewage sludge strains of Eubacterium limosum, a methanol- and H2-CO2-utilizing species.
journal, January 1981
- Genthner, B. R.; Davis, C. L.; Bryant, M. P.
- Applied and Environmental Microbiology, Vol. 42, Issue 1
Phascolarctobacterium faecium gen. nov, spec. nov., a Novel Taxon of the Sporomusa Group of Bacteria
journal, November 1993
- Del Dot, Therese; Osawa, Ro; Stackebrandt, Erko
- Systematic and Applied Microbiology, Vol. 16, Issue 3
Change in microbial communities in acetate- and glucose-fed microbial fuel cells in the presence of light
journal, September 2009
- Xing, Defeng; Cheng, Shaoan; Regan, John M.
- Biosensors and Bioelectronics, Vol. 25, Issue 1
Bio-electrochemical property and phylogenetic diversity of microbial communities associated with bioelectrodes of an electromethanogenic reactor
journal, July 2013
- Kobayashi, Hajime; Saito, Naoki; Fu, Qian
- Journal of Bioscience and Bioengineering, Vol. 116, Issue 1
Death by a thousand cuts: the challenges and diverse landscape of lignocellulosic hydrolysate inhibitors
journal, March 2014
- Piotrowski, Jeff S.; Zhang, Yaoping; Bates, Donna M.
- Frontiers in Microbiology, Vol. 5
Towards sustainable wastewater treatment by using microbial fuel cells-centered technologies
journal, November 2013
- Li, Wen-Wei; Yu, Han-Qing; He, Zhen
- Energy Environ. Sci., Vol. 7, Issue 3
Microbial electrolysis cells turning to be versatile technology: Recent advances and future challenges
journal, June 2014
- Zhang, Yifeng; Angelidaki, Irini
- Water Research, Vol. 56
Treatability studies on different refinery wastewater samples using high-throughput microbial electrolysis cells (MECs)
journal, May 2013
- Ren, Lijiao; Siegert, Michael; Ivanov, Ivan
- Bioresource Technology, Vol. 136
Nutrients removal and recovery in bioelectrochemical systems: A review
journal, February 2014
- Kelly, Patrick T.; He, Zhen
- Bioresource Technology, Vol. 153
Conversion of Residual Organics in Corn Stover-Derived Biorefinery Stream to Bioenergy via a Microbial Fuel Cell
journal, August 2012
- Borole, Abhijeet P.; Hamilton, Choo Y.; Schell, Daniel J.
- Environmental Science & Technology, Vol. 47, Issue 1
Cloacibacillus evryensis gen. nov., sp. nov., a novel asaccharolytic, mesophilic, amino-acid-degrading bacterium within the phylum 'Synergistetes', isolated from an anaerobic sludge digester
journal, September 2008
- Ganesan, A.; Chaussonnerie, S.; Tarrade, A.
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Vol. 58, Issue 9
Effect of Nitrate Reduction on the Microbial Reductive Transformation of Pentachloronitrobenzene
journal, May 2008
- Okutman Tas, Didem; Pavlostathis, Spyros G.
- Environmental Science & Technology, Vol. 42, Issue 9
Hydrogen production from cellulose in a two-stage process combining fermentation and electrohydrogenesis
journal, August 2009
- Lalaurette, Elodie; Thammannagowda, Shivegowda; Mohagheghi, Ali
- International Journal of Hydrogen Energy, Vol. 34, Issue 15
Fate of H 2 in an Upflow Single-Chamber Microbial Electrolysis Cell Using a Metal-Catalyst-Free Cathode
journal, October 2009
- Lee, Hyung-Sool; Torres, César I.; Parameswaran, Prathap
- Environmental Science & Technology, Vol. 43, Issue 20
Geobacter sulfurreducens sp. nov., a hydrogen- and acetate-oxidizing dissimilatory metal-reducing microorganism.
journal, January 1994
- Caccavo, F.; Lonergan, D. J.; Lovley, D. R.
- Applied and Environmental Microbiology, Vol. 60, Issue 10
Works referencing / citing this record:
Advances in energy systems for valorization of aqueous byproducts generated from hydrothermal processing of biomass and systems thinking
journal, January 2019
- Gu, Yexuan; Zhang, Xuesong; Deal, Brian
- Green Chemistry, Vol. 21, Issue 10
Unravelling biocomplexity of electroactive biofilms for producing hydrogen from biomass
journal, July 2017
- Lewis, Alex J.; Campa, Maria F.; Hazen, Terry C.
- Microbial Biotechnology, Vol. 11, Issue 1
Evaluation of gas and carbon transport in a methanogenic bioelectrochemical system (BES): Gas and Carbon Transport in a Methanogenic BES
journal, December 2016
- Dykstra, Christy M.; Pavlostathis, Spyros G.
- Biotechnology and Bioengineering, Vol. 114, Issue 5
Biological hydrogen production: molecular and electrolytic perspectives
journal, July 2019
- Mahidhara, Ganesh; Burrow, Hannah; Sasikala, Ch.
- World Journal of Microbiology and Biotechnology, Vol. 35, Issue 8
Sustainable hydrothermal self-assembly of hafnium–lignosulfonate nanohybrids for highly efficient reductive upgrading of 5-hydroxymethylfurfural
journal, January 2019
- Zhou, Shenghui; Dai, Fanglin; Chen, Yian
- Green Chemistry, Vol. 21, Issue 6
Processes and electron flow in a microbial electrolysis cell bioanode fed with furanic and phenolic compounds
journal, March 2018
- Zeng, Xiaofei; Borole, Abhijeet P.; Pavlostathis, Spyros G.
- Environmental Science and Pollution Research, Vol. 25, Issue 36
Characterization and utilization of aqueous products from hydrothermal conversion of biomass for bio-oil and hydro-char production: a review
journal, January 2019
- Usman, Muhammad; Chen, Huihui; Chen, Kaifei
- Green Chemistry, Vol. 21, Issue 7
Glucose and Applied Voltage Accelerated p-Nitrophenol Reduction in Biocathode of Bioelectrochemical Systems
journal, March 2018
- Wang, Xinyu; Xing, Defeng; Mei, Xiaoxue
- Frontiers in Microbiology, Vol. 9