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Title: The proteome and phosphoproteome of Neurospora crassa in response to cellulose, sucrose and carbon starvation

Abstract

Improving cellulolytic enzyme production by plant biomass degrading fungi holds great potential in reducing costs associated with production of next-generation biofuels generated from lignocellulose. How fungi sense cellulosic materials and respond by secreting enzymes has mainly been examined by assessing function of transcriptional regulators and via transcriptional profiling. Here, we obtained global proteomic and phosphoproteomic profiles of the plant biomass degrading filamentous fungus Neurospora crassa grown on different carbon sources, i.e. sucrose, no carbon, and cellulose, by performing isobaric tags for relative and absolute quantification (iTRAQ)-based LC-MS/MS analyses. A comparison between proteomes and transcriptomes under identical carbon conditions suggests that extensive post-transcriptional regulation occurs in N. crassa in response to exposure to cellulosic material. Several hundred amino acid residues with differential phosphorylation levels on crystalline cellulose (Avicel) or carbon-free medium vs sucrose medium were identified, including phosphorylation sites in a major transcriptional activator for cellulase genes, CLR1, as well as a cellobionic acid transporter, CBT1. Mutation of phosphorylation sites on CLR1 did not have a major effect on transactivation of cellulase production, while mutation of phosphorylation sites in CBT1 increased its transporting capacity. Our data provides rich information at both the protein and phosphorylation levels of the early cellular responsesmore » to carbon starvation and cellulosic induction and aids in a greater understanding of the underlying post-transcriptional regulatory mechanisms in filamentous fungi.« less

Authors:
ORCiD logo [1];  [1];  [2];  [3];  [3];  [3];  [3];  [3];  [4];  [3];  [1]
  1. Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology
  2. Univ. of California, Berkeley, CA (United States). California Inst. for Quantitative Biosciences
  3. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  4. Univ. of California, Berkeley, CA (United States). Dept. of Molecular and Cell Biology; Univ. of California, Berkeley, CA (United States). Dept. of Chemistry
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1188945
Alternate Identifier(s):
OSTI ID: 1222386; OSTI ID: 1407259
Report Number(s):
PNNL-SA-103196
Journal ID: ISSN 1087-1845; 48135; 400412000
Grant/Contract Number:  
AC05-76RL01830; P41GM103493; AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
Fungal Genetics and Biology
Additional Journal Information:
Journal Volume: 72; Journal Issue: C; Journal ID: ISSN 1087-1845
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; Neurospora crassa; Proteome; Phosphoproteome; Cellulase; Carbon starvation; Plant biomass; Environmental Molecular Sciences Laboratory

Citation Formats

Xiong, Yi, Coradetti, Samuel T., Li, Xin, Gritsenko, Marina A., Clauss, Therese, Petyuk, Vlad, Camp, David, Smith, Richard, Cate, Jamie H. D., Yang, Feng, and Glass, N. Louise. The proteome and phosphoproteome of Neurospora crassa in response to cellulose, sucrose and carbon starvation. United States: N. p., 2014. Web. doi:10.1016/j.fgb.2014.05.005.
Xiong, Yi, Coradetti, Samuel T., Li, Xin, Gritsenko, Marina A., Clauss, Therese, Petyuk, Vlad, Camp, David, Smith, Richard, Cate, Jamie H. D., Yang, Feng, & Glass, N. Louise. The proteome and phosphoproteome of Neurospora crassa in response to cellulose, sucrose and carbon starvation. United States. https://doi.org/10.1016/j.fgb.2014.05.005
Xiong, Yi, Coradetti, Samuel T., Li, Xin, Gritsenko, Marina A., Clauss, Therese, Petyuk, Vlad, Camp, David, Smith, Richard, Cate, Jamie H. D., Yang, Feng, and Glass, N. Louise. Thu . "The proteome and phosphoproteome of Neurospora crassa in response to cellulose, sucrose and carbon starvation". United States. https://doi.org/10.1016/j.fgb.2014.05.005. https://www.osti.gov/servlets/purl/1188945.
@article{osti_1188945,
title = {The proteome and phosphoproteome of Neurospora crassa in response to cellulose, sucrose and carbon starvation},
author = {Xiong, Yi and Coradetti, Samuel T. and Li, Xin and Gritsenko, Marina A. and Clauss, Therese and Petyuk, Vlad and Camp, David and Smith, Richard and Cate, Jamie H. D. and Yang, Feng and Glass, N. Louise},
abstractNote = {Improving cellulolytic enzyme production by plant biomass degrading fungi holds great potential in reducing costs associated with production of next-generation biofuels generated from lignocellulose. How fungi sense cellulosic materials and respond by secreting enzymes has mainly been examined by assessing function of transcriptional regulators and via transcriptional profiling. Here, we obtained global proteomic and phosphoproteomic profiles of the plant biomass degrading filamentous fungus Neurospora crassa grown on different carbon sources, i.e. sucrose, no carbon, and cellulose, by performing isobaric tags for relative and absolute quantification (iTRAQ)-based LC-MS/MS analyses. A comparison between proteomes and transcriptomes under identical carbon conditions suggests that extensive post-transcriptional regulation occurs in N. crassa in response to exposure to cellulosic material. Several hundred amino acid residues with differential phosphorylation levels on crystalline cellulose (Avicel) or carbon-free medium vs sucrose medium were identified, including phosphorylation sites in a major transcriptional activator for cellulase genes, CLR1, as well as a cellobionic acid transporter, CBT1. Mutation of phosphorylation sites on CLR1 did not have a major effect on transactivation of cellulase production, while mutation of phosphorylation sites in CBT1 increased its transporting capacity. Our data provides rich information at both the protein and phosphorylation levels of the early cellular responses to carbon starvation and cellulosic induction and aids in a greater understanding of the underlying post-transcriptional regulatory mechanisms in filamentous fungi.},
doi = {10.1016/j.fgb.2014.05.005},
journal = {Fungal Genetics and Biology},
number = C,
volume = 72,
place = {United States},
year = {2014},
month = {5}
}

Journal Article:

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Cited by: 26 works
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