The proteome and phosphoproteome of Neurospora crassa in response to cellulose, sucrose and carbon starvation
Abstract
Improving cellulolytic enzyme production by plant biomass degrading fungi holds great potential in reducing costs associated with production of next-generation biofuels generated from lignocellulose. How fungi sense cellulosic materials and respond by secreting enzymes has mainly been examined by assessing function of transcriptional regulators and via transcriptional profiling. Here, we obtained global proteomic and phosphoproteomic profiles of the plant biomass degrading filamentous fungus Neurospora crassa grown on different carbon sources, i.e. sucrose, no carbon, and cellulose, by performing isobaric tags for relative and absolute quantification (iTRAQ)-based LC-MS/MS analyses. A comparison between proteomes and transcriptomes under identical carbon conditions suggests that extensive post-transcriptional regulation occurs in N. crassa in response to exposure to cellulosic material. Several hundred amino acid residues with differential phosphorylation levels on crystalline cellulose (Avicel) or carbon-free medium vs sucrose medium were identified, including phosphorylation sites in a major transcriptional activator for cellulase genes, CLR1, as well as a cellobionic acid transporter, CBT1. Mutation of phosphorylation sites on CLR1 did not have a major effect on transactivation of cellulase production, while mutation of phosphorylation sites in CBT1 increased its transporting capacity. Our data provides rich information at both the protein and phosphorylation levels of the early cellular responsesmore »
- Authors:
-
- Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology
- Univ. of California, Berkeley, CA (United States). California Inst. for Quantitative Biosciences
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Univ. of California, Berkeley, CA (United States). Dept. of Molecular and Cell Biology; Univ. of California, Berkeley, CA (United States). Dept. of Chemistry
- Publication Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States). Environmental Molecular Sciences Laboratory (EMSL); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1188945
- Alternate Identifier(s):
- OSTI ID: 1222386; OSTI ID: 1407259
- Report Number(s):
- PNNL-SA-103196
Journal ID: ISSN 1087-1845; 48135; 400412000
- Grant/Contract Number:
- AC05-76RL01830; P41GM103493; AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Fungal Genetics and Biology
- Additional Journal Information:
- Journal Volume: 72; Journal Issue: C; Journal ID: ISSN 1087-1845
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; Neurospora crassa; Proteome; Phosphoproteome; Cellulase; Carbon starvation; Plant biomass; Environmental Molecular Sciences Laboratory
Citation Formats
Xiong, Yi, Coradetti, Samuel T., Li, Xin, Gritsenko, Marina A., Clauss, Therese, Petyuk, Vlad, Camp, David, Smith, Richard, Cate, Jamie H. D., Yang, Feng, and Glass, N. Louise. The proteome and phosphoproteome of Neurospora crassa in response to cellulose, sucrose and carbon starvation. United States: N. p., 2014.
Web. doi:10.1016/j.fgb.2014.05.005.
Xiong, Yi, Coradetti, Samuel T., Li, Xin, Gritsenko, Marina A., Clauss, Therese, Petyuk, Vlad, Camp, David, Smith, Richard, Cate, Jamie H. D., Yang, Feng, & Glass, N. Louise. The proteome and phosphoproteome of Neurospora crassa in response to cellulose, sucrose and carbon starvation. United States. https://doi.org/10.1016/j.fgb.2014.05.005
Xiong, Yi, Coradetti, Samuel T., Li, Xin, Gritsenko, Marina A., Clauss, Therese, Petyuk, Vlad, Camp, David, Smith, Richard, Cate, Jamie H. D., Yang, Feng, and Glass, N. Louise. Thu .
"The proteome and phosphoproteome of Neurospora crassa in response to cellulose, sucrose and carbon starvation". United States. https://doi.org/10.1016/j.fgb.2014.05.005. https://www.osti.gov/servlets/purl/1188945.
@article{osti_1188945,
title = {The proteome and phosphoproteome of Neurospora crassa in response to cellulose, sucrose and carbon starvation},
author = {Xiong, Yi and Coradetti, Samuel T. and Li, Xin and Gritsenko, Marina A. and Clauss, Therese and Petyuk, Vlad and Camp, David and Smith, Richard and Cate, Jamie H. D. and Yang, Feng and Glass, N. Louise},
abstractNote = {Improving cellulolytic enzyme production by plant biomass degrading fungi holds great potential in reducing costs associated with production of next-generation biofuels generated from lignocellulose. How fungi sense cellulosic materials and respond by secreting enzymes has mainly been examined by assessing function of transcriptional regulators and via transcriptional profiling. Here, we obtained global proteomic and phosphoproteomic profiles of the plant biomass degrading filamentous fungus Neurospora crassa grown on different carbon sources, i.e. sucrose, no carbon, and cellulose, by performing isobaric tags for relative and absolute quantification (iTRAQ)-based LC-MS/MS analyses. A comparison between proteomes and transcriptomes under identical carbon conditions suggests that extensive post-transcriptional regulation occurs in N. crassa in response to exposure to cellulosic material. Several hundred amino acid residues with differential phosphorylation levels on crystalline cellulose (Avicel) or carbon-free medium vs sucrose medium were identified, including phosphorylation sites in a major transcriptional activator for cellulase genes, CLR1, as well as a cellobionic acid transporter, CBT1. Mutation of phosphorylation sites on CLR1 did not have a major effect on transactivation of cellulase production, while mutation of phosphorylation sites in CBT1 increased its transporting capacity. Our data provides rich information at both the protein and phosphorylation levels of the early cellular responses to carbon starvation and cellulosic induction and aids in a greater understanding of the underlying post-transcriptional regulatory mechanisms in filamentous fungi.},
doi = {10.1016/j.fgb.2014.05.005},
journal = {Fungal Genetics and Biology},
number = C,
volume = 72,
place = {United States},
year = {Thu May 29 00:00:00 EDT 2014},
month = {Thu May 29 00:00:00 EDT 2014}
}
Web of Science
Works referenced in this record:
A comparative systems analysis of polysaccharide-elicited responses in Neurospora crassa reveals carbon source-specific cellular adaptations : Fungal polysaccharide-specific responses revealed
journal, December 2013
- Benz, J. Philipp; Chau, Bryant H.; Zheng, Diana
- Molecular Microbiology, Vol. 91, Issue 2
Identification and characterization of a galacturonic acid transporter from Neurospora crassa and its application for Saccharomyces cerevisiae fermentation processes
journal, January 2014
- Benz, J.; Protzko, Ryan J.; Andrich, Jonas MS
- Biotechnology for Biofuels, Vol. 7, Issue 1
Xyr1 regulates xylanase but not cellulase formation in the head blight fungus Fusarium graminearum
journal, October 2007
- Brunner, Kurt; Lichtenauer, Anton M.; Kratochwill, Klaus
- Current Genetics, Vol. 52, Issue 5-6
Proteomics-based Compositional Analysis of Complex Cellulase–Hemicellulase Mixtures
journal, October 2011
- Chundawat, Shishir P. S.; Lipton, Mary S.; Purvine, Samuel O.
- Journal of Proteome Research, Vol. 10, Issue 10
The regulation of protein function by multisite phosphorylation – a 25 year update
journal, December 2000
- Cohen, P.
- Trends in Biochemical Sciences, Vol. 25, Issue 12
Conserved and essential transcription factors for cellulase gene expression in ascomycete fungi
journal, April 2012
- Coradetti, S. T.; Craig, J. P.; Xiong, Y.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 19
Analysis of a conserved cellulase transcriptional regulator reveals inducer-independent production of cellulolytic enzymes in Neurospora crassa
journal, June 2013
- Coradetti, Samuel T.; Xiong, Yi; Glass, N. Louise
- MicrobiologyOpen, Vol. 2, Issue 4
Phosphorylation Positively Regulates DNA Binding of the Carbon Catabolite Repressor Cre1 of Hypocrea jecorina ( Trichoderma reesei )
journal, February 2002
- Cziferszky, Angela; Mach, Robert L.; Kubicek, Christian P.
- Journal of Biological Chemistry, Vol. 277, Issue 17
Genetic Evidence for the Heterodimeric Structure of Glucosidase II
journal, September 1999
- D'Alessio, Cecilia; Fernández, Fabiana; Trombetta, E. Sergio
- Journal of Biological Chemistry, Vol. 274, Issue 36
Comprehensive mass-spectrometry-based proteome quantification of haploid versus diploid yeast
journal, September 2008
- de Godoy, Lyris M. F.; Olsen, Jesper V.; Cox, Jürgen
- Nature, Vol. 455, Issue 7217
Proteomic Analysis of the Secretory Response of Aspergillus niger to D-Maltose and D-Xylose
journal, June 2011
- Ferreira de Oliveira, José Miguel P.; van Passel, Mark W. J.; Schaap, Peter J.
- PLoS ONE, Vol. 6, Issue 6
Uncovering the Genome-Wide Transcriptional Responses of the Filamentous Fungus Aspergillus niger to Lignocellulose Using RNA Sequencing
journal, August 2012
- Delmas, Stéphane; Pullan, Steven T.; Gaddipati, Sanyasi
- PLoS Genetics, Vol. 8, Issue 8
Mutation of the Xylanase regulator 1 causes a glucose blind hydrolase expressing phenotype in industrially used Trichoderma strains
journal, January 2013
- Derntl, Christian; Gudynaite-Savitch, Loreta; Calixte, Sophie
- Biotechnology for Biofuels, Vol. 6, Issue 1
Secretome analysis of the fungus Trichoderma harzianum grown on cellulose
journal, August 2012
- Do Vale, Luis H. F.; Gómez-Mendoza, Diana P.; Kim, Min-Sik
- PROTEOMICS, Vol. 12, Issue 17
Carbon Catabolite Repression of Gene Expression and Conidiation inNeurospora crassa
journal, October 1998
- Ebbole, Daniel J.
- Fungal Genetics and Biology, Vol. 25, Issue 1
An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database
journal, November 1994
- Eng, Jimmy K.; McCormack, Ashley L.; Yates, John R.
- Journal of the American Society for Mass Spectrometry, Vol. 5, Issue 11
Comparative Protein Structure Modeling Using MODELLER
journal, November 2007
- Eswar, Narayanan; Webb, Ben; Marti-Renom, Marc A.
- Current Protocols in Protein Science, Vol. 50, Issue 1
Principles of Carbon Catabolite Repression in the Rice Blast Fungus: Tps1, Nmr1-3, and a MATE–Family Pump Regulate Glucose Metabolism during Infection
journal, May 2012
- Fernandez, Jessie; Wright, Janet D.; Hartline, David
- PLoS Genetics, Vol. 8, Issue 5
Magnetic Bead Processor for Rapid Evaluation and Optimization of Parameters for Phosphopeptide Enrichment
journal, June 2009
- Ficarro, Scott B.; Adelmant, Guillaume; Tomar, Maria N.
- Analytical Chemistry, Vol. 81, Issue 11
Delayed Correlation of mRNA and Protein Expression in Rapamycin-treated Cells and a Role for Ggc1 in Cellular Sensitivity to Rapamycin
journal, November 2009
- Fournier, Marjorie L.; Paulson, Ariel; Pavelka, Norman
- Molecular & Cellular Proteomics, Vol. 9, Issue 2
Cellodextrin Transport in Yeast for Improved Biofuel Production
journal, September 2010
- Galazka, J. M.; Tian, C.; Beeson, W. T.
- Science, Vol. 330, Issue 6000
Comparative secretome analyses of two Trichoderma reesei RUT-C30 and CL847 hypersecretory strains
journal, January 2008
- Herpoël-Gimbert, Isabelle; Margeot, Antoine; Dolla, Alain
- Biotechnology for Biofuels, Vol. 1, Issue 1
Signaling by Target of Rapamycin Proteins in Cell Growth Control
journal, March 2005
- Inoki, Ken; Ouyang, Hongjiao; Li, Yong
- Microbiology and Molecular Biology Reviews, Vol. 69, Issue 1
Predicting transmembrane protein topology with a hidden markov model: application to complete genomes11Edited by F. Cohen
journal, January 2001
- Krogh, Anders; Larsson, Björn; von Heijne, Gunnar
- Journal of Molecular Biology, Vol. 305, Issue 3
Microbial Cellulases and Their Industrial Applications
journal, January 2011
- Kuhad, Ramesh Chander; Gupta, Rishi; Singh, Ajay
- Enzyme Research, Vol. 2011
Molecular taxonomy of the yeasts
journal, December 1994
- Kurtzman, Cletus P.
- Yeast, Vol. 10, Issue 13
Tracking the roots of cellulase hyperproduction by the fungus Trichoderma reesei using massively parallel DNA sequencing
journal, September 2009
- Le Crom, S.; Schackwitz, W.; Pennacchio, L.
- Proceedings of the National Academy of Sciences, Vol. 106, Issue 38
A dynamic model of proteome changes reveals new roles for transcript alteration in yeast
journal, January 2011
- Lee, M. Violet; Topper, Scott E.; Hubler, Shane L.
- Molecular Systems Biology, Vol. 7, Issue 1
Secretome diversity and quantitative analysis of cellulolytic Aspergillus fumigatus Z5 in the presence of different carbon sources
journal, January 2013
- Liu, Dongyang; Li, Juan; Zhao, Shuang
- Biotechnology for Biofuels, Vol. 6, Issue 1
Long-term strain improvements accumulate mutations in regulatory elements responsible for hyper-production of cellulolytic enzymes
journal, March 2013
- Liu, Guodong; Zhang, Lei; Qin, Yuqi
- Scientific Reports, Vol. 3, Issue 1
Two TOR Complexes, Only One of which Is Rapamycin Sensitive, Have Distinct Roles in Cell Growth Control
journal, September 2002
- Loewith, Robbie; Jacinto, Estela; Wullschleger, Stephan
- Molecular Cell, Vol. 10, Issue 3
Defining the transcriptome and proteome in three functionally different human cell lines
journal, January 2010
- Lundberg, Emma; Fagerberg, Linn; Klevebring, Daniel
- Molecular Systems Biology, Vol. 6, Issue 1
Transcriptional Regulation of xyr1, Encoding the Main Regulator of the Xylanolytic and Cellulolytic Enzyme System in Hypocrea jecorina
journal, September 2008
- Mach-Aigner, A. R.; Pucher, M. E.; Steiger, M. G.
- Applied and Environmental Microbiology, Vol. 74, Issue 21
Correlation of mRNA and protein in complex biological samples
journal, October 2009
- Maier, Tobias; Güell, Marc; Serrano, Luis
- FEBS Letters, Vol. 583, Issue 24
The Protein Kinase Complement of the Human Genome
journal, December 2002
- Manning, G.
- Science, Vol. 298, Issue 5600
Isolation and characterization of a Neurospora glucose-repressible gene
journal, December 1988
- McNally, Mark T.; Free, Stephen J.
- Current Genetics, Vol. 14, Issue 6
MASIC: A software program for fast quantitation and flexible visualization of chromatographic profiles from detected LC–MS(/MS) features
journal, June 2008
- Monroe, Matthew E.; Shaw, Jason L.; Daly, Don S.
- Computational Biology and Chemistry, Vol. 32, Issue 3
Quantitative Phosphoproteomic Analysis of Soybean Root Hairs Inoculated with Bradyrhizobium japonicum
journal, July 2012
- Nguyen, Tran Hong Nha; Brechenmacher, Laurent; Aldrich, Joshua T.
- Molecular & Cellular Proteomics, Vol. 11, Issue 11
Xylose Triggers Reversible Phosphorylation of XlnR, the Fungal Transcriptional Activator of Xylanolytic and Cellulolytic Genes in Aspergillus oryzae
journal, May 2011
- Noguchi, Yuji; Tanaka, Hisaki; Kanamaru, Kyoko
- Bioscience, Biotechnology, and Biochemistry, Vol. 75, Issue 5
ManR, a Transcriptional Regulator of the β-Mannan Utilization System, Controls the Cellulose Utilization System in <i>Aspergillus oryzae</i>
journal, January 2013
- Ogawa, Masahiro; Kobayashi, Tetsuo; Koyama, Yasuji
- Bioscience, Biotechnology, and Biochemistry, Vol. 77, Issue 2
A Functional GTPase Domain, but not its Transmembrane Domain, is Required for Function of the SRP Receptor β-subunit
journal, July 1998
- Ogg, Stephen C.; Barz, Wolfgang P.; Walter, Peter
- Journal of Cell Biology, Vol. 142, Issue 2
Quantitative Proteomic Approach for Cellulose Degradation by Neurospora crassa
journal, September 2011
- Phillips, Christopher M.; Iavarone, Anthony T.; Marletta, Michael A.
- Journal of Proteome Research, Vol. 10, Issue 9
Single Nucleotide Polymorphism Analysis of a Trichoderma reesei Hyper-Cellulolytic Mutant Developed in Japan
journal, March 2013
- Porciuncula, Juliano de Oliveira; Furukawa, Takanori; Mori, Kazuki
- Bioscience, Biotechnology, and Biochemistry, Vol. 77, Issue 3
Protein translocation across the eukaryotic endoplasmic reticulum and bacterial plasma membranes
journal, November 2007
- Rapoport, Tom A.
- Nature, Vol. 450, Issue 7170
Genome-wide transcriptional response of Trichoderma reesei to lignocellulose using RNA sequencing and comparison with Aspergillus niger
journal, January 2013
- Ries, Laure; Pullan, Steven T.; Delmas, Stéphane
- BMC Genomics, Vol. 14, Issue 1
The FunCat, a functional annotation scheme for systematic classification of proteins from whole genomes
journal, October 2004
- Ruepp, A.
- Nucleic Acids Research, Vol. 32, Issue 18
GAL4 is phosphorylated as a consequence of transcriptional activation.
journal, December 1991
- Sadowski, I.; Niedbala, D.; Wood, K.
- Proceedings of the National Academy of Sciences, Vol. 88, Issue 23
Global quantification of mammalian gene expression control
journal, May 2011
- Schwanhäusser, Björn; Busse, Dorothea; Li, Na
- Nature, Vol. 473, Issue 7347
Human-Like Eukaryotic Translation Initiation Factor 3 from Neurospora crassa
journal, November 2013
- Smith, M. Duane; Gu, Yu; Querol-Audí, Jordi
- PLoS ONE, Vol. 8, Issue 11
Effects of Calnexin Deletion in Saccharomyces cerevisiae on the Secretion of Glycosylated Lysozymes
journal, December 2001
- Song, Y.; Sata, J.; Saito, A.
- Journal of Biochemistry, Vol. 130, Issue 6
Regulation of transcription of cellulases- and hemicellulases-encoding genes in Aspergillus niger and Hypocrea jecorina (Trichoderma reesei)
journal, February 2008
- Stricker, Astrid R.; Mach, Robert L.; de Graaff, Leo H.
- Applied Microbiology and Biotechnology, Vol. 78, Issue 2
Crystal structure of a bacterial homologue of glucose transporters GLUT1–4
journal, October 2012
- Sun, Linfeng; Zeng, Xin; Yan, Chuangye
- Nature, Vol. 490, Issue 7420
Complex regulation of hydrolytic enzyme genes for cellulosic biomass degradation in filamentous fungi
journal, April 2014
- Tani, Shuji; Kawaguchi, Takashi; Kobayashi, Tetsuo
- Applied Microbiology and Biotechnology, Vol. 98, Issue 11
Quantifying E. coli Proteome and Transcriptome with Single-Molecule Sensitivity in Single Cells
journal, July 2010
- Taniguchi, Y.; Choi, P. J.; Li, G. -W.
- Science, Vol. 329, Issue 5991
The Transcriptional Activator XlnR Regulates Both Xylanolytic and Endoglucanase Gene Expression inAspergillus niger
journal, October 1998
- van Peij, Noël N. M. E.; Gielkens, Marco M. C.; de Vries, Ronald P.
- Applied and Environmental Microbiology, Vol. 64, Issue 10
Sequence signatures and mRNA concentration can explain two‐thirds of protein abundance variation in a human cell line
journal, January 2010
- Vogel, Christine; de Sousa Abreu, Raquel; Ko, Daijin
- Molecular Systems Biology, Vol. 6, Issue 1
Insights into the regulation of protein abundance from proteomic and transcriptomic analyses
journal, March 2012
- Vogel, Christine; Marcotte, Edward M.
- Nature Reviews Genetics, Vol. 13, Issue 4
The Unfolded Protein Response: From Stress Pathway to Homeostatic Regulation
journal, November 2011
- Walter, P.; Ron, D.
- Science, Vol. 334, Issue 6059
Reversed-phase chromatography with multiple fraction concatenation strategy for proteome profiling of human MCF10A cells
journal, April 2011
- Wang, Yuexi; Yang, Feng; Gritsenko, Marina A.
- PROTEOMICS, Vol. 11, Issue 10
Transcriptional response to glucose starvation and functional analysis of a glucose transporter of Neurospora crassa
journal, December 2004
- Xie, Xin; Wilkinson, Heather H.; Correa, Alejandro
- Fungal Genetics and Biology, Vol. 41, Issue 12
Induction of lignocellulose-degrading enzymes in Neurospora crassa by cellodextrins
journal, April 2012
- Znameroski, E. A.; Coradetti, S. T.; Roche, C. M.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 16
Evidence for Transceptor Function of Cellodextrin Transporters in Neurospora crassa
journal, December 2013
- Znameroski, Elizabeth A.; Li, Xin; Tsai, Jordan C.
- Journal of Biological Chemistry, Vol. 289, Issue 5
Works referencing / citing this record:
Direct Target Network of the Neurospora crassa Plant Cell Wall Deconstruction Regulators CLR-1, CLR-2, and XLR-1
journal, October 2015
- Craig, James P.; Coradetti, Samuel T.; Starr, Trevor L.
- mBio, Vol. 6, Issue 5
Consolidated bioprocessing of lignocellulosic biomass to itaconic acid by metabolically engineering Neurospora crassa
journal, September 2018
- Zhao, Chen; Chen, Shaolin; Fang, Hao
- Applied Microbiology and Biotechnology, Vol. 102, Issue 22
A fungal transcription factor essential for starch degradation affects integration of carbon and nitrogen metabolism
journal, May 2017
- Xiong, Yi; Wu, Vincent W.; Lubbe, Andrea
- PLOS Genetics, Vol. 13, Issue 5
Network reconstruction and systems analysis of plant cell wall deconstruction by Neurospora crassa
journal, September 2017
- Samal, Areejit; Craig, James P.; Coradetti, Samuel T.
- Biotechnology for Biofuels, Vol. 10, Issue 1
The Putative Cellodextrin Transporter-like Protein CLP1 Is Involved in Cellulase Induction in Neurospora crassa
journal, November 2014
- Cai, Pengli; Wang, Bang; Ji, Jingxiao
- Journal of Biological Chemistry, Vol. 290, Issue 2
Broad Substrate-Specific Phosphorylation Events Are Associated With the Initial Stage of Plant Cell Wall Recognition in Neurospora crassa
journal, November 2019
- Horta, Maria Augusta C.; Thieme, Nils; Gao, Yuqian
- Frontiers in Microbiology, Vol. 10
Network of nutrient-sensing pathways and a conserved kinase cascade integrate osmolarity and carbon sensing in Neurospora crassa
journal, September 2017
- Huberman, Lori B.; Coradetti, Samuel T.; Glass, N. Louise
- Proceedings of the National Academy of Sciences, Vol. 114, Issue 41
Biodegradation of Cellulose in Laboratory-Scale Bioreactors: Experimental and Numerical Studies
journal, September 2019
- Mistriotis, Antonis; Papardaki, Nikoleta-Georgia; Provata, Astero
- Journal of Polymers and the Environment, Vol. 27, Issue 12
mus-52 disruption and metabolic regulation in Neurospora crassa: Transcriptional responses to extracellular phosphate availability
journal, April 2018
- Martins, Maíra P.; Gomes, Eriston V.; Sanches, Pablo R.
- PLOS ONE, Vol. 13, Issue 4
Biotechnological production of value-added compounds by ustilaginomycetous yeasts
journal, September 2017
- Paulino, Bruno N.; Pessôa, Marina G.; Molina, Gustavo
- Applied Microbiology and Biotechnology, Vol. 101, Issue 21
STK-12 acts as a transcriptional brake to control the expression of cellulase-encoding genes in Neurospora crassa
journal, November 2019
- Lin, Liangcai; Wang, Shanshan; Li, Xiaolin
- PLOS Genetics, Vol. 15, Issue 11
Regulation of Cell-to-Cell Communication and Cell Wall Integrity by a Network of MAP Kinase Pathways and Transcription Factors in Neurospora crassa
journal, April 2018
- Fischer, Monika S.; Wu, Vincent W.; Lee, Ji E.
- Genetics, Vol. 209, Issue 2
Expanding xylose metabolism in yeast for plant cell wall conversion to biofuels
journal, February 2015
- Li, Xin; Yu, Vivian Yaci; Lin, Yuping
- eLife, Vol. 4
Identification and characterization of the glucose dual-affinity transport system in Neurospora crassa: pleiotropic roles in nutrient transport, signaling, and carbon catabolite repression
journal, January 2017
- Wang, Bang; Li, Jingen; Gao, Jingfang
- Biotechnology for Biofuels, Vol. 10, Issue 1
Cellobionic acid utilization: from Neurospora crassa to Saccharomyces cerevisiae
journal, August 2015
- Li, Xin; Chomvong, Kulika; Yu, Vivian Yaci
- Biotechnology for Biofuels, Vol. 8, Issue 1
Direct Target Network of the Neurospora crassa Plant Cell Wall Deconstruction Regulators CLR-1, CLR-2, and XLR-1
journal, October 2015
- Craig, James P.; Coradetti, Samuel T.; Starr, Trevor L.
- mBio, Vol. 6, Issue 5
VIB1, a Link between Glucose Signaling and Carbon Catabolite Repression, Is Essential for Plant Cell Wall Degradation by Neurospora crassa
journal, August 2014
- Xiong, Yi; Sun, Jianping; Glass, N. Louise
- PLoS Genetics, Vol. 10, Issue 8
A fungal transcription factor essential for starch degradation affects integration of carbon and nitrogen metabolism
journal, May 2017
- Xiong, Yi; Wu, Vincent W.; Lubbe, Andrea
- PLOS Genetics, Vol. 13, Issue 5
STK-12 acts as a transcriptional brake to control the expression of cellulase-encoding genes in Neurospora crassa
journal, November 2019
- Lin, Liangcai; Wang, Shanshan; Li, Xiaolin
- PLOS Genetics, Vol. 15, Issue 11
Proteomic and Phosphoproteomic Insights into a Signaling Hub Role for Cdc14 in Asexual Development and Multiple Stress Responses in Beauveria bassiana
journal, April 2016
- Wang, Zhi-Kang; Wang, Jie; Liu, Jing
- PLOS ONE, Vol. 11, Issue 4
Communicate and Fuse: How Filamentous Fungi Establish and Maintain an Interconnected Mycelial Network
journal, March 2019
- Fischer, Monika S.; Glass, N. Louise
- Frontiers in Microbiology, Vol. 10