The functional potential of microbial communities in hydraulic fracturing source water and produced water from natural gas extraction characterized by metagenomic sequencing
Abstract
Microbial activity in produced water from hydraulic fracturing operations can lead to undesired environmental impacts and increase gas production costs. However, the metabolic profile of these microbial communities is not well understood. Here, for the first time, we present results from a shotgun metagenome of microbial communities in both hydraulic fracturing source water and wastewater produced by hydraulic fracturing. Taxonomic analyses showed an increase in anaerobic/facultative anaerobic classes related to Clostridia, Gammaproteobacteria, Bacteroidia and Epsilonproteobacteria in produced water as compared to predominantly aerobic Alphaproteobacteria in the fracturing source water. Thus, the metabolic profile revealed a relative increase in genes responsible for carbohydrate metabolism, respiration, sporulation and dormancy, iron acquisition and metabolism, stress response and sulfur metabolism in the produced water samples. These results suggest that microbial communities in produced water have an increased genetic ability to handle stress, which has significant implications for produced water management, such as disinfection.
- Authors:
-
- National Energy Technology Lab., Pittsburgh, PA (United States); Carnegie Mellon Univ., Pittsburgh, PA (United States)
- National Energy Technology Lab., Pittsburgh, PA (United States); Univ. of Pittsburgh, Pittsburgh, PA (United States)
- National Energy Technology Lab., Pittsburgh, PA (United States)
- Agriculture and Agri-Food Canada (Canada)
- Publication Date:
- Research Org.:
- National Energy Technology Laboratory (NETL), Pittsburgh, PA, Morgantown, WV (United States)
- Sponsoring Org.:
- USDOE Office of Fossil Energy (FE)
- OSTI Identifier:
- 1168804
- Report Number(s):
- NETL-PUB-1113
Journal ID: ISSN 1932-6203
- Resource Type:
- Accepted Manuscript
- Journal Name:
- PLoS ONE
- Additional Journal Information:
- Journal Volume: 9; Journal Issue: 10; Journal ID: ISSN 1932-6203
- Publisher:
- Public Library of Science
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 54 ENVIRONMENTAL SCIENCES; carbohydrate metabolism; sulfur; ribosomal RNA; sequence assembly tools; metagenomics; gene mapping; natural gas; sequence analysis
Citation Formats
Mohan, Arvind Murali, Bibby, Kyle J., Lipus, Daniel, Hammack, Richard W., Gregory, Kelvin B., and Forster, Robert J. The functional potential of microbial communities in hydraulic fracturing source water and produced water from natural gas extraction characterized by metagenomic sequencing. United States: N. p., 2014.
Web. doi:10.1371/journal.pone.0107682.
Mohan, Arvind Murali, Bibby, Kyle J., Lipus, Daniel, Hammack, Richard W., Gregory, Kelvin B., & Forster, Robert J. The functional potential of microbial communities in hydraulic fracturing source water and produced water from natural gas extraction characterized by metagenomic sequencing. United States. https://doi.org/10.1371/journal.pone.0107682
Mohan, Arvind Murali, Bibby, Kyle J., Lipus, Daniel, Hammack, Richard W., Gregory, Kelvin B., and Forster, Robert J. Wed .
"The functional potential of microbial communities in hydraulic fracturing source water and produced water from natural gas extraction characterized by metagenomic sequencing". United States. https://doi.org/10.1371/journal.pone.0107682. https://www.osti.gov/servlets/purl/1168804.
@article{osti_1168804,
title = {The functional potential of microbial communities in hydraulic fracturing source water and produced water from natural gas extraction characterized by metagenomic sequencing},
author = {Mohan, Arvind Murali and Bibby, Kyle J. and Lipus, Daniel and Hammack, Richard W. and Gregory, Kelvin B. and Forster, Robert J.},
abstractNote = {Microbial activity in produced water from hydraulic fracturing operations can lead to undesired environmental impacts and increase gas production costs. However, the metabolic profile of these microbial communities is not well understood. Here, for the first time, we present results from a shotgun metagenome of microbial communities in both hydraulic fracturing source water and wastewater produced by hydraulic fracturing. Taxonomic analyses showed an increase in anaerobic/facultative anaerobic classes related to Clostridia, Gammaproteobacteria, Bacteroidia and Epsilonproteobacteria in produced water as compared to predominantly aerobic Alphaproteobacteria in the fracturing source water. Thus, the metabolic profile revealed a relative increase in genes responsible for carbohydrate metabolism, respiration, sporulation and dormancy, iron acquisition and metabolism, stress response and sulfur metabolism in the produced water samples. These results suggest that microbial communities in produced water have an increased genetic ability to handle stress, which has significant implications for produced water management, such as disinfection.},
doi = {10.1371/journal.pone.0107682},
journal = {PLoS ONE},
number = 10,
volume = 9,
place = {United States},
year = {Wed Oct 22 00:00:00 EDT 2014},
month = {Wed Oct 22 00:00:00 EDT 2014}
}
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