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Title: Metagenomic Analysis of Subtidal Sediments from Polar and Subpolar Coastal Environments Highlights the Relevance of Anaerobic Hydrocarbon Degradation Processes

Abstract

In this work, we analyzed the community structure and metabolic potential of sediment microbial communities in high-latitude coastal environments subjected to low to moderate levels of chronic pollution. Subtidal sediments from four low-energy inlets located in polar and subpolar regions from both Hemispheres were analyzed using large-scale 16S rRNA gene and metagenomic sequencing. Communities showed high diversity (Shannon’s index 6.8 to 10.2), with distinct phylogenetic structures (<40% shared taxa at the Phylum level among regions) but similar metabolic potential in terms of sequences assigned to KOs. Environmental factors (mainly salinity, temperature, and in less extent organic pollution) were drivers of both phylogenetic and functional traits. Bacterial taxa correlating with hydrocarbon pollution included families of anaerobic or facultative anaerobic lifestyle, such as Desulfuromonadaceae, Geobacteraceae, and Rhodocyclaceae. In accordance, biomarker genes for anaerobic hydrocarbon degradation (bamA, ebdA, bcrA, and bssA) were prevalent, only outnumbered by alkB, and their sequences were taxonomically binned to the same bacterial groups. BssA-assigned metagenomic sequences showed an extremely wide diversity distributed all along the phylogeny known for this gene, including bssA sensu stricto, nmsA, assA, and other clusters from poorly or not yet described variants. This work increases our understanding of microbial community patterns in cold coastalmore » sediments, and highlights the relevance of anaerobic hydrocarbon degradation processes in subtidal environments.« less

Authors:
; ; ; ; ; ; ; ; ORCiD logo
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1437533
Report Number(s):
PNNL-SA-127251
Journal ID: ISSN 0095-3628
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Microbial Ecology
Additional Journal Information:
Journal Volume: 75; Journal Issue: 1; Journal ID: ISSN 0095-3628
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
tidal; sediment; metagenomic

Citation Formats

Espínola, Fernando, Dionisi, Hebe M., Borglin, Sharon, Brislawn, Colin J., Jansson, Janet K., Mac Cormack, Walter P., Carroll, JoLynn, Sjöling, Sara, and Lozada, Mariana. Metagenomic Analysis of Subtidal Sediments from Polar and Subpolar Coastal Environments Highlights the Relevance of Anaerobic Hydrocarbon Degradation Processes. United States: N. p., 2017. Web. doi:10.1007/s00248-017-1028-5.
Espínola, Fernando, Dionisi, Hebe M., Borglin, Sharon, Brislawn, Colin J., Jansson, Janet K., Mac Cormack, Walter P., Carroll, JoLynn, Sjöling, Sara, & Lozada, Mariana. Metagenomic Analysis of Subtidal Sediments from Polar and Subpolar Coastal Environments Highlights the Relevance of Anaerobic Hydrocarbon Degradation Processes. United States. doi:10.1007/s00248-017-1028-5.
Espínola, Fernando, Dionisi, Hebe M., Borglin, Sharon, Brislawn, Colin J., Jansson, Janet K., Mac Cormack, Walter P., Carroll, JoLynn, Sjöling, Sara, and Lozada, Mariana. Wed . "Metagenomic Analysis of Subtidal Sediments from Polar and Subpolar Coastal Environments Highlights the Relevance of Anaerobic Hydrocarbon Degradation Processes". United States. doi:10.1007/s00248-017-1028-5.
@article{osti_1437533,
title = {Metagenomic Analysis of Subtidal Sediments from Polar and Subpolar Coastal Environments Highlights the Relevance of Anaerobic Hydrocarbon Degradation Processes},
author = {Espínola, Fernando and Dionisi, Hebe M. and Borglin, Sharon and Brislawn, Colin J. and Jansson, Janet K. and Mac Cormack, Walter P. and Carroll, JoLynn and Sjöling, Sara and Lozada, Mariana},
abstractNote = {In this work, we analyzed the community structure and metabolic potential of sediment microbial communities in high-latitude coastal environments subjected to low to moderate levels of chronic pollution. Subtidal sediments from four low-energy inlets located in polar and subpolar regions from both Hemispheres were analyzed using large-scale 16S rRNA gene and metagenomic sequencing. Communities showed high diversity (Shannon’s index 6.8 to 10.2), with distinct phylogenetic structures (<40% shared taxa at the Phylum level among regions) but similar metabolic potential in terms of sequences assigned to KOs. Environmental factors (mainly salinity, temperature, and in less extent organic pollution) were drivers of both phylogenetic and functional traits. Bacterial taxa correlating with hydrocarbon pollution included families of anaerobic or facultative anaerobic lifestyle, such as Desulfuromonadaceae, Geobacteraceae, and Rhodocyclaceae. In accordance, biomarker genes for anaerobic hydrocarbon degradation (bamA, ebdA, bcrA, and bssA) were prevalent, only outnumbered by alkB, and their sequences were taxonomically binned to the same bacterial groups. BssA-assigned metagenomic sequences showed an extremely wide diversity distributed all along the phylogeny known for this gene, including bssA sensu stricto, nmsA, assA, and other clusters from poorly or not yet described variants. This work increases our understanding of microbial community patterns in cold coastal sediments, and highlights the relevance of anaerobic hydrocarbon degradation processes in subtidal environments.},
doi = {10.1007/s00248-017-1028-5},
journal = {Microbial Ecology},
issn = {0095-3628},
number = 1,
volume = 75,
place = {United States},
year = {2017},
month = {7}
}

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