Predominance and high diversity of genes associated to denitrification in metagenomes of subantarctic coastal sediments exposed to urban pollution

Calderoli, Priscila A.; Espínola, Fernando J.; Dionisi, Hebe M.; Gil, Mónica N.; Jansson, Janet K.; Lozada, Mariana

doi:10.1371/journal.pone.0207606

Predominance and high diversity of genes associated to denitrification in metagenomes of subantarctic coastal sediments exposed to urban pollution

Journal Article · Wed Nov 28 23:00:00 EST 2018 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0207606· OSTI ID:1489274

Calderoli, Priscila A. ^[1]; Espínola, Fernando J. ^[1]; Dionisi, Hebe M. ^[1]; Gil, Mónica N. ^[2]; Jansson, Janet K. ^[3]; ^[1]

Centro para el Estudio de Sistemas Marinos, Puerto Madryn (Argentina)
Centro para el Estudio de Sistemas Marinos, Puerto Madryn (Argentina); CCT CONICET CENPAT, Puerto Madryn (Argentina)
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

The aim of this work was to characterize the microbial nitrogen cycling potential in sediments from Ushuaia Bay, a subantarctic environment that has suffered a recent explosive demographic growth. Subtidal sediment samples were retrieved in triplicate from two urban points in the Bay, and analyzed through metagenomic shotgun sequencing. Sequences assigned to genes related to nitrification, nitrate reduction and denitrification were predominant in this environment with respect to metagenomes from other environments, including other marine sediments. The nosZ gene, responsible for nitrous oxide transformation into di-nitrogen, presented a high diversity. The majority of NosZ sequences were classified as Clade II (atypical) variants affiliated to different bacterial lineages such as Bacteroidetes, Chloroflexi, Firmicutes, Proteobacteria, Verrucomicrobia, as well as to Archaea. The analysis of a fosmid metagenomic library from the same site showed that the genomic context of atypical variants was variable, and was accompanied by distinct regulatory elements, suggesting the evolution of differential ecophysiological roles. This work increases our understanding of the microbial ecology of nitrogen transformations in cold coastal environments and provides evidence of an enhanced denitrification potential in impacted sediment microbial communities. In addition, it highlights the role of yet overlooked populations in the mitigation of environmentally harmful forms of nitrogen.

View Accepted Manuscript (DOE)

Research Organization:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 1489274

Report Number(s):: PNNL-SA--125133

Journal Information:: PLoS ONE, Journal Name: PLoS ONE Journal Issue: 11 Vol. 13; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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