Abstract
Resveratrol, a polyphenol in red wine, possesses many pharmacological activities including cardio-protection, chemoprevention, anti-tumor effects, and nuclear factor-kappa B (NF-{kappa}B) inactivation. The present study was designed to evaluate the effects and possible mechanism of resveratrol in enhancing radiosensitivity of lung cancer cells. Human non-small cell lung cancer NCI-H838 cells were irradiated with or without resveratrol pretreatment. The surviving fraction and sensitizer enhancement ratio (SER) were estimated by using a colony formation assay and linear-quadratic model. The cell-cycle distribution was evaluated by using prospidium iodide staining and flow cytometry. An enzyme-linked immunosorbent assay (ELISA)-based assay with immobilized oligonucleotide was performed to assess the DNA binding activity of NF-{kappa}B. Resveratrol had no direct growth-inhibitory effect on NCI-H838 cells treated for 24 hours with doses up to 25 {mu}M. Pretreatment with resveratrol significantly enhanced cell killing by radiation, with an SER up to 2.2. Radiation activated NF-{kappa}B, an effect reversed by resveratrol pretreatment. Resveratrol resulted in a decrease of cells in the G{sub 0}/G{sub 1} phase and an increase in the S phase. Our results demonstrate that resveratrol enhances the radiosensitivity of NCI-H838 cells accompanied by NF-{kappa}B inhibition and S-phase arrest. (author)
Liao, Hui-Fen;
[1]
Cheng-Deng, Kuo;
[2]
Yang, Yuh-Cheng;
Lin, Chin-Ping;
Tai, Hung-Chi;
Chen, Yu-Jen;
[3]
Chen, Yu-Yawn
[4]
- National Chiayi Univ., Chiayi, Taiwan (China)
- Taipei Veterans General Hospital, Taiwan (China)
- Mackay Memorial Hospital, Taipei, Taiwan (China)
- National Taiwan Coll. of Physical Education, Graduate School of Physical Education, Taichung, Taiwan (China)
Citation Formats
Liao, Hui-Fen, Cheng-Deng, Kuo, Yang, Yuh-Cheng, Lin, Chin-Ping, Tai, Hung-Chi, Chen, Yu-Jen, and Chen, Yu-Yawn.
Resveratrol enhances radiosensitivity of human non-small cell lung cancer NCI-H838 cells accompanied by inhibition of nuclear factor-kappa B activation.
Japan: N. p.,
2005.
Web.
doi:10.1269/jrr.46.387.
Liao, Hui-Fen, Cheng-Deng, Kuo, Yang, Yuh-Cheng, Lin, Chin-Ping, Tai, Hung-Chi, Chen, Yu-Jen, & Chen, Yu-Yawn.
Resveratrol enhances radiosensitivity of human non-small cell lung cancer NCI-H838 cells accompanied by inhibition of nuclear factor-kappa B activation.
Japan.
https://doi.org/10.1269/jrr.46.387
Liao, Hui-Fen, Cheng-Deng, Kuo, Yang, Yuh-Cheng, Lin, Chin-Ping, Tai, Hung-Chi, Chen, Yu-Jen, and Chen, Yu-Yawn.
2005.
"Resveratrol enhances radiosensitivity of human non-small cell lung cancer NCI-H838 cells accompanied by inhibition of nuclear factor-kappa B activation."
Japan.
https://doi.org/10.1269/jrr.46.387.
@misc{etde_20739174,
title = {Resveratrol enhances radiosensitivity of human non-small cell lung cancer NCI-H838 cells accompanied by inhibition of nuclear factor-kappa B activation}
author = {Liao, Hui-Fen, Cheng-Deng, Kuo, Yang, Yuh-Cheng, Lin, Chin-Ping, Tai, Hung-Chi, Chen, Yu-Jen, and Chen, Yu-Yawn}
abstractNote = {Resveratrol, a polyphenol in red wine, possesses many pharmacological activities including cardio-protection, chemoprevention, anti-tumor effects, and nuclear factor-kappa B (NF-{kappa}B) inactivation. The present study was designed to evaluate the effects and possible mechanism of resveratrol in enhancing radiosensitivity of lung cancer cells. Human non-small cell lung cancer NCI-H838 cells were irradiated with or without resveratrol pretreatment. The surviving fraction and sensitizer enhancement ratio (SER) were estimated by using a colony formation assay and linear-quadratic model. The cell-cycle distribution was evaluated by using prospidium iodide staining and flow cytometry. An enzyme-linked immunosorbent assay (ELISA)-based assay with immobilized oligonucleotide was performed to assess the DNA binding activity of NF-{kappa}B. Resveratrol had no direct growth-inhibitory effect on NCI-H838 cells treated for 24 hours with doses up to 25 {mu}M. Pretreatment with resveratrol significantly enhanced cell killing by radiation, with an SER up to 2.2. Radiation activated NF-{kappa}B, an effect reversed by resveratrol pretreatment. Resveratrol resulted in a decrease of cells in the G{sub 0}/G{sub 1} phase and an increase in the S phase. Our results demonstrate that resveratrol enhances the radiosensitivity of NCI-H838 cells accompanied by NF-{kappa}B inhibition and S-phase arrest. (author)}
doi = {10.1269/jrr.46.387}
journal = []
issue = {4}
volume = {46}
place = {Japan}
year = {2005}
month = {Dec}
}
title = {Resveratrol enhances radiosensitivity of human non-small cell lung cancer NCI-H838 cells accompanied by inhibition of nuclear factor-kappa B activation}
author = {Liao, Hui-Fen, Cheng-Deng, Kuo, Yang, Yuh-Cheng, Lin, Chin-Ping, Tai, Hung-Chi, Chen, Yu-Jen, and Chen, Yu-Yawn}
abstractNote = {Resveratrol, a polyphenol in red wine, possesses many pharmacological activities including cardio-protection, chemoprevention, anti-tumor effects, and nuclear factor-kappa B (NF-{kappa}B) inactivation. The present study was designed to evaluate the effects and possible mechanism of resveratrol in enhancing radiosensitivity of lung cancer cells. Human non-small cell lung cancer NCI-H838 cells were irradiated with or without resveratrol pretreatment. The surviving fraction and sensitizer enhancement ratio (SER) were estimated by using a colony formation assay and linear-quadratic model. The cell-cycle distribution was evaluated by using prospidium iodide staining and flow cytometry. An enzyme-linked immunosorbent assay (ELISA)-based assay with immobilized oligonucleotide was performed to assess the DNA binding activity of NF-{kappa}B. Resveratrol had no direct growth-inhibitory effect on NCI-H838 cells treated for 24 hours with doses up to 25 {mu}M. Pretreatment with resveratrol significantly enhanced cell killing by radiation, with an SER up to 2.2. Radiation activated NF-{kappa}B, an effect reversed by resveratrol pretreatment. Resveratrol resulted in a decrease of cells in the G{sub 0}/G{sub 1} phase and an increase in the S phase. Our results demonstrate that resveratrol enhances the radiosensitivity of NCI-H838 cells accompanied by NF-{kappa}B inhibition and S-phase arrest. (author)}
doi = {10.1269/jrr.46.387}
journal = []
issue = {4}
volume = {46}
place = {Japan}
year = {2005}
month = {Dec}
}