{
  "date" : "2005-12-15",
  "identifier_doecontract" : "",
  "subject" : "63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; BIOLOGICAL RADIATION EFFECTS; CELL CULTURES; CELL CYCLE; DOSE-RESPONSE RELATIONSHIPS; ELECTRON BEAMS; FLUORESCENCE SPECTROSCOPY; LINEAR ACCELERATORS; LUNGS; MORPHOLOGICAL CHANGES; POLYPHENOLS; RADIATION DOSES; RADIOSENSITIVITY; SURVIVAL CURVES; TUMOR CELLS; VIABILITY",
  "description" : "Resveratrol, a polyphenol in red wine, possesses many pharmacological activities including cardio-protection, chemoprevention, anti-tumor effects, and nuclear factor-kappa B (NF-{kappa}B) inactivation. The present study was designed to evaluate the effects and possible mechanism of resveratrol in enhancing radiosensitivity of lung cancer cells. Human non-small cell lung cancer NCI-H838 cells were irradiated with or without resveratrol pretreatment. The surviving fraction and sensitizer enhancement ratio (SER) were estimated by using a colony formation assay and linear-quadratic model. The cell-cycle distribution was evaluated by using prospidium iodide staining and flow cytometry. An enzyme-linked immunosorbent assay (ELISA)-based assay with immobilized oligonucleotide was performed to assess the DNA binding activity of NF-{kappa}B. Resveratrol had no direct growth-inhibitory effect on NCI-H838 cells treated for 24 hours with doses up to 25 {mu}M. Pretreatment with resveratrol significantly enhanced cell killing by radiation, with an SER up to 2.2. Radiation activated NF-{kappa}B, an effect reversed by resveratrol pretreatment. Resveratrol resulted in a decrease of cells in the G{sub 0}/G{sub 1} phase and an increase in the S phase. Our results demonstrate that resveratrol enhances the radiosensitivity of NCI-H838 cells accompanied by NF-{kappa}B inhibition and S-phase arrest. (author)",
  "language" : "English",
  "identifier_report" : "",
  "publisher_sponsor" : "",
  "publisher_country" : "Japan",
  "source" : "INIS",
  "purl" : "",
  "title" : "Resveratrol enhances radiosensitivity of human non-small cell lung cancer NCI-H838 cells accompanied by inhibition of nuclear factor-kappa B activation",
  "type" : "Journal Article",
  "subject_related" : "",
  "relation" : "Journal Name: Journal of Radiation Research; Journal Volume: 46; Journal Issue: 4",
  "entry_date" : "2010-12-31",
  "subject_list" : [ "63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS", "BIOLOGICAL RADIATION EFFECTS", "CELL CULTURES", "CELL CYCLE", "DOSE-RESPONSE RELATIONSHIPS", "ELECTRON BEAMS", "FLUORESCENCE SPECTROSCOPY", "LINEAR ACCELERATORS", "LUNGS", "MORPHOLOGICAL CHANGES", "POLYPHENOLS", "RADIATION DOSES", "RADIOSENSITIVITY", "SURVIVAL CURVES", "TUMOR CELLS", "VIABILITY" ],
  "publisher_availability" : "",
  "rights" : "",
  "announced_date" : "2006-06-19",
  "type_qualifier" : "",
  "has_fulltext" : false,
  "coverage" : "",
  "identifier" : "",
  "journal_volume" : "46",
  "creator" : "Liao, Hui-Fen [National Chiayi Univ., Chiayi, Taiwan (China)]; Cheng-Deng, Kuo [Taipei Veterans General Hospital, Taiwan (China)]; Yang, Yuh-Cheng; Lin, Chin-Ping; Tai, Hung-Chi; Chen, Yu-Jen [Mackay Memorial Hospital, Taipei, Taiwan (China)]; Chen, Yu-Yawn [National Taiwan Coll. of Physical Education, Graduate School of Physical Education, Taichung, Taiwan (China)]",
  "site_ownership_code" : "INIS",
  "osti_id" : "20739174",
  "journal_issue" : "4",
  "resource_type" : "JOUR",
  "format" : "Medium: X; Size: page(s) 387-393",
  "journal_name" : [ ],
  "contributing_organizations" : "",
  "citation_location" : "https://www.osti.gov/etdeweb/biblio/20739174",
  "publication_year" : 2005,
  "subject_list_commas" : "63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS, BIOLOGICAL RADIATION EFFECTS, CELL CULTURES, CELL CYCLE, DOSE-RESPONSE RELATIONSHIPS, ELECTRON BEAMS, FLUORESCENCE SPECTROSCOPY, LINEAR ACCELERATORS, LUNGS, MORPHOLOGICAL CHANGES, POLYPHENOLS, RADIATION DOSES, RADIOSENSITIVITY, SURVIVAL CURVES, TUMOR CELLS, VIABILITY",
  "publisher" : "",
  "identifier_other" : "Journal ID: ISSN 0449-3060; JRARAX; TRN: JP0601359048968",
  "publisher_research" : "",
  "creators_list" : [ "Liao, Hui-Fen [National Chiayi Univ., Chiayi, Taiwan (China)]", "Cheng-Deng, Kuo [Taipei Veterans General Hospital, Taiwan (China)]", "Yang, Yuh-Cheng", "Lin, Chin-Ping", "Tai, Hung-Chi", "Chen, Yu-Jen [Mackay Memorial Hospital, Taipei, Taiwan (China)]", "Chen, Yu-Yawn [National Taiwan Coll. of Physical Education, Graduate School of Physical Education, Taichung, Taiwan (China)]" ],
  "doi" : "https://doi.org/10.1269/jrr.46.387"
}