Resveratrol enhances radiosensitivity of human non-small cell lung cancer NCI-H838 cells accompanied by inhibition of nuclear factor-kappa B activation Liao, Hui-Fen [National Chiayi Univ., Chiayi, Taiwan (China)]; Cheng-Deng, Kuo [Taipei Veterans General Hospital, Taiwan (China)]; Yang, Yuh-Cheng; Lin, Chin-Ping; Tai, Hung-Chi; Chen, Yu-Jen [Mackay Memorial Hospital, Taipei, Taiwan (China)]; Chen, Yu-Yawn [National Taiwan Coll. of Physical Education, Graduate School of Physical Education, Taichung, Taiwan (China)] 63 RADIATION, THERMAL, AND OTHER ENVIRONMENTAL POLLUTANT EFFECTS ON LIVING ORGANISMS AND BIOLOGICAL MATERIALS; BIOLOGICAL RADIATION EFFECTS; CELL CULTURES; CELL CYCLE; DOSE-RESPONSE RELATIONSHIPS; ELECTRON BEAMS; FLUORESCENCE SPECTROSCOPY; LINEAR ACCELERATORS; LUNGS; MORPHOLOGICAL CHANGES; POLYPHENOLS; RADIATION DOSES; RADIOSENSITIVITY; SURVIVAL CURVES; TUMOR CELLS; VIABILITY Resveratrol, a polyphenol in red wine, possesses many pharmacological activities including cardio-protection, chemoprevention, anti-tumor effects, and nuclear factor-kappa B (NF-{kappa}B) inactivation. The present study was designed to evaluate the effects and possible mechanism of resveratrol in enhancing radiosensitivity of lung cancer cells. Human non-small cell lung cancer NCI-H838 cells were irradiated with or without resveratrol pretreatment. The surviving fraction and sensitizer enhancement ratio (SER) were estimated by using a colony formation assay and linear-quadratic model. The cell-cycle distribution was evaluated by using prospidium iodide staining and flow cytometry. An enzyme-linked immunosorbent assay (ELISA)-based assay with immobilized oligonucleotide was performed to assess the DNA binding activity of NF-{kappa}B. Resveratrol had no direct growth-inhibitory effect on NCI-H838 cells treated for 24 hours with doses up to 25 {mu}M. Pretreatment with resveratrol significantly enhanced cell killing by radiation, with an SER up to 2.2. Radiation activated NF-{kappa}B, an effect reversed by resveratrol pretreatment. Resveratrol resulted in a decrease of cells in the G{sub 0}/G{sub 1} phase and an increase in the S phase. Our results demonstrate that resveratrol enhances the radiosensitivity of NCI-H838 cells accompanied by NF-{kappa}B inhibition and S-phase arrest. (author) Japan 2005-12-15 English Journal Article Journal Name: Journal of Radiation Research; Journal Volume: 46; Journal Issue: 4 Medium: X; Size: page(s) 387-393 https://doi.org/10.1269/jrr.46.387 [] 4 46 Journal ID: ISSN 0449-3060; JRARAX; TRN: JP0601359048968 INIS 2010-12-31 20739174